Cargando…

Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells

As the main bioactive component in dried ginger, 6-shogaol has potential hypoglycemic activity, but its mechanism is still unclear. The process of carbohydrate digestion and glucose absorption is closely related to the enzymatic activity of epithelial brush cells, expression of glucose transporters,...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiao, Wenya, Sang, Yaxin, Wang, Xianghong, Wang, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913893/
https://www.ncbi.nlm.nih.gov/pubmed/36766032
http://dx.doi.org/10.3390/foods12030503
_version_ 1784885536001884160
author Jiao, Wenya
Sang, Yaxin
Wang, Xianghong
Wang, Shuo
author_facet Jiao, Wenya
Sang, Yaxin
Wang, Xianghong
Wang, Shuo
author_sort Jiao, Wenya
collection PubMed
description As the main bioactive component in dried ginger, 6-shogaol has potential hypoglycemic activity, but its mechanism is still unclear. The process of carbohydrate digestion and glucose absorption is closely related to the enzymatic activity of epithelial brush cells, expression of glucose transporters, and permeability of intestinal epithelial cells. Therefore, this study explored the hypoglycemic mechanism of 6-shogaol from the perspective of glucose uptake, absorption transport, and protection of intestinal barrier function. Based on molecular docking, the binding energy of 6-shogaol and α-glucosidase is −6.24 kcal/mol, showing a high binding affinity. Moreover, a-glucosidase enzymatic activity was reduced (−78.96%) when the 6-shogaol concentration was 500 µg/mL. After 6-shogaol intervention, the glucose uptake was reduced; the relative expression of glucose transporters GLUT2 and SGLT1 were down regulated; and tight junction proteins ZO-1, Occludin and Claudin were up regulated in differentiated Caco-2 cells. This study confirmed that 6-shogaol effectively inhibits the activity of α-glucosidase and has beneficial effects on glucose uptake, protection of intestinal barrier function, and promotion of intestinal material absorption.
format Online
Article
Text
id pubmed-9913893
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99138932023-02-11 Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells Jiao, Wenya Sang, Yaxin Wang, Xianghong Wang, Shuo Foods Article As the main bioactive component in dried ginger, 6-shogaol has potential hypoglycemic activity, but its mechanism is still unclear. The process of carbohydrate digestion and glucose absorption is closely related to the enzymatic activity of epithelial brush cells, expression of glucose transporters, and permeability of intestinal epithelial cells. Therefore, this study explored the hypoglycemic mechanism of 6-shogaol from the perspective of glucose uptake, absorption transport, and protection of intestinal barrier function. Based on molecular docking, the binding energy of 6-shogaol and α-glucosidase is −6.24 kcal/mol, showing a high binding affinity. Moreover, a-glucosidase enzymatic activity was reduced (−78.96%) when the 6-shogaol concentration was 500 µg/mL. After 6-shogaol intervention, the glucose uptake was reduced; the relative expression of glucose transporters GLUT2 and SGLT1 were down regulated; and tight junction proteins ZO-1, Occludin and Claudin were up regulated in differentiated Caco-2 cells. This study confirmed that 6-shogaol effectively inhibits the activity of α-glucosidase and has beneficial effects on glucose uptake, protection of intestinal barrier function, and promotion of intestinal material absorption. MDPI 2023-01-21 /pmc/articles/PMC9913893/ /pubmed/36766032 http://dx.doi.org/10.3390/foods12030503 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiao, Wenya
Sang, Yaxin
Wang, Xianghong
Wang, Shuo
Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title_full Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title_fullStr Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title_full_unstemmed Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title_short Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells
title_sort effects of 6-shogaol on glucose uptake and intestinal barrier integrity in caco-2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913893/
https://www.ncbi.nlm.nih.gov/pubmed/36766032
http://dx.doi.org/10.3390/foods12030503
work_keys_str_mv AT jiaowenya effectsof6shogaolonglucoseuptakeandintestinalbarrierintegrityincaco2cells
AT sangyaxin effectsof6shogaolonglucoseuptakeandintestinalbarrierintegrityincaco2cells
AT wangxianghong effectsof6shogaolonglucoseuptakeandintestinalbarrierintegrityincaco2cells
AT wangshuo effectsof6shogaolonglucoseuptakeandintestinalbarrierintegrityincaco2cells