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Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods

Norfloxacin (NOR) is a common antibiotic used in humans and animals, and its high levels can cause intolerance or poisoning. Therefore, NOR levels in animal-derived foods must be monitored due to potential side effects and illegal use phenomena. This research centered on the development of an enviro...

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Autores principales: Chen, Shujuan, Zhu, Yiting, Han, Jing, Zhang, Tianyi, Chou, Runwen, Liu, Aiping, Liu, Shuliang, Yang, Yong, Hu, Kaidi, Zou, Likou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913916/
https://www.ncbi.nlm.nih.gov/pubmed/36766073
http://dx.doi.org/10.3390/foods12030544
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author Chen, Shujuan
Zhu, Yiting
Han, Jing
Zhang, Tianyi
Chou, Runwen
Liu, Aiping
Liu, Shuliang
Yang, Yong
Hu, Kaidi
Zou, Likou
author_facet Chen, Shujuan
Zhu, Yiting
Han, Jing
Zhang, Tianyi
Chou, Runwen
Liu, Aiping
Liu, Shuliang
Yang, Yong
Hu, Kaidi
Zou, Likou
author_sort Chen, Shujuan
collection PubMed
description Norfloxacin (NOR) is a common antibiotic used in humans and animals, and its high levels can cause intolerance or poisoning. Therefore, NOR levels in animal-derived foods must be monitored due to potential side effects and illegal use phenomena. This research centered on the development of an environmentally friendly electrochemical sensor for NOR detection. Potassium carbonate activated tea branch biochar (K-TBC) as an efficient use of waste was coated on the surface of glassy carbon electrode (GCE), and a molecular-imprinted polymer (MIP) layer was subsequently electropolymerized onto the modified electrode. NOR was used as template molecule and o-phenylenediamine (o-PD) and o-aminophenol (o-AP) were used as bifunctional monomers. The electrochemical sensor was built and its electrochemical behavior on NOR was investigated. The sensor demonstrated an excellent linear current response to NOR concentrations in the ranges of 0.1–0.5 nM and 0.5–100 nM under ideal experimental circumstances, with a detection limit of 0.028 nM (S/N = 3). With recoveries ranging from 85.90% to 101.71%, the designed sensor was effectively used to detect NOR in actual samples of milk, honey, and pork. Besides, the fabricated sensor had low price, short detection time, good selectivity and stability, which can provide a theoretical and practical basis for the actual monitoring of NOR residues.
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spelling pubmed-99139162023-02-11 Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods Chen, Shujuan Zhu, Yiting Han, Jing Zhang, Tianyi Chou, Runwen Liu, Aiping Liu, Shuliang Yang, Yong Hu, Kaidi Zou, Likou Foods Article Norfloxacin (NOR) is a common antibiotic used in humans and animals, and its high levels can cause intolerance or poisoning. Therefore, NOR levels in animal-derived foods must be monitored due to potential side effects and illegal use phenomena. This research centered on the development of an environmentally friendly electrochemical sensor for NOR detection. Potassium carbonate activated tea branch biochar (K-TBC) as an efficient use of waste was coated on the surface of glassy carbon electrode (GCE), and a molecular-imprinted polymer (MIP) layer was subsequently electropolymerized onto the modified electrode. NOR was used as template molecule and o-phenylenediamine (o-PD) and o-aminophenol (o-AP) were used as bifunctional monomers. The electrochemical sensor was built and its electrochemical behavior on NOR was investigated. The sensor demonstrated an excellent linear current response to NOR concentrations in the ranges of 0.1–0.5 nM and 0.5–100 nM under ideal experimental circumstances, with a detection limit of 0.028 nM (S/N = 3). With recoveries ranging from 85.90% to 101.71%, the designed sensor was effectively used to detect NOR in actual samples of milk, honey, and pork. Besides, the fabricated sensor had low price, short detection time, good selectivity and stability, which can provide a theoretical and practical basis for the actual monitoring of NOR residues. MDPI 2023-01-26 /pmc/articles/PMC9913916/ /pubmed/36766073 http://dx.doi.org/10.3390/foods12030544 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Shujuan
Zhu, Yiting
Han, Jing
Zhang, Tianyi
Chou, Runwen
Liu, Aiping
Liu, Shuliang
Yang, Yong
Hu, Kaidi
Zou, Likou
Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title_full Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title_fullStr Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title_full_unstemmed Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title_short Construction of a Molecularly Imprinted Sensor Modified with Tea Branch Biochar and Its Rapid Detection of Norfloxacin Residues in Animal-Derived Foods
title_sort construction of a molecularly imprinted sensor modified with tea branch biochar and its rapid detection of norfloxacin residues in animal-derived foods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913916/
https://www.ncbi.nlm.nih.gov/pubmed/36766073
http://dx.doi.org/10.3390/foods12030544
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