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Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA

Zika virus (ZIKV) diagnostics are crucial for proper antenatal and postnatal care and also for surveillance and serosurvey studies. Since the viremia during ZIKV infection is fleeting, serological testing is highly valuable to inform diagnosis. However, current serology tests using whole virus antig...

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Autores principales: Ndiaye, Oumar, Diagne, Cheikh Tidiane, Abd El Wahed, Ahmed, Dia, Fatou, Dia, Moussa, Faye, Adama, Leal, Silvania Da Veiga, dos Santos, Menilita, de Lima Mendonça, Maria da Luz, da Silva Leite, Carolina Cardoso, Bouh Boye, Cheikh Saad, Bryant, Juliet E., Desprès, Philippe, Faye, Ousmane, Sall, Amadou Alpha, Faye, Oumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913938/
https://www.ncbi.nlm.nih.gov/pubmed/36766567
http://dx.doi.org/10.3390/diagnostics13030462
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author Ndiaye, Oumar
Diagne, Cheikh Tidiane
Abd El Wahed, Ahmed
Dia, Fatou
Dia, Moussa
Faye, Adama
Leal, Silvania Da Veiga
dos Santos, Menilita
de Lima Mendonça, Maria da Luz
da Silva Leite, Carolina Cardoso
Bouh Boye, Cheikh Saad
Bryant, Juliet E.
Desprès, Philippe
Faye, Ousmane
Sall, Amadou Alpha
Faye, Oumar
author_facet Ndiaye, Oumar
Diagne, Cheikh Tidiane
Abd El Wahed, Ahmed
Dia, Fatou
Dia, Moussa
Faye, Adama
Leal, Silvania Da Veiga
dos Santos, Menilita
de Lima Mendonça, Maria da Luz
da Silva Leite, Carolina Cardoso
Bouh Boye, Cheikh Saad
Bryant, Juliet E.
Desprès, Philippe
Faye, Ousmane
Sall, Amadou Alpha
Faye, Oumar
author_sort Ndiaye, Oumar
collection PubMed
description Zika virus (ZIKV) diagnostics are crucial for proper antenatal and postnatal care and also for surveillance and serosurvey studies. Since the viremia during ZIKV infection is fleeting, serological testing is highly valuable to inform diagnosis. However, current serology tests using whole virus antigens frequently suffer from cross reactivity issues, delays, and technical complexity, especially in low and middle income countries (LMICs) and endemic countries. Here, we describe an indirect ELISA to detect specific IgG antibodies using the ZIKV envelope domain III (EDIII) protein expressed in Drosophila S2 cells as an immunogen. Using a total of 367 clinical samples, we showed that the EDIII-ELISA was able to detect IgG antibodies against ZIKV with high sensitivity of 100.0% and specificity of 94.7% when compared to plaque reduction neutralization tests (PRNTs) as the gold standard and using 0.208 as the cut-off OD value. These results show the usefulness of the recombinant envelope domain III as an alternative to standard whole virus proteins for ZIKV diagnostics as it improves the sensitivity and specificity of IgG ELISA assay when used as an immunogen. This method should, therefore, be extended to serological diagnostic techniques for other members of the flavivirus genus and for use in IgM diagnostic testing.
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spelling pubmed-99139382023-02-11 Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA Ndiaye, Oumar Diagne, Cheikh Tidiane Abd El Wahed, Ahmed Dia, Fatou Dia, Moussa Faye, Adama Leal, Silvania Da Veiga dos Santos, Menilita de Lima Mendonça, Maria da Luz da Silva Leite, Carolina Cardoso Bouh Boye, Cheikh Saad Bryant, Juliet E. Desprès, Philippe Faye, Ousmane Sall, Amadou Alpha Faye, Oumar Diagnostics (Basel) Article Zika virus (ZIKV) diagnostics are crucial for proper antenatal and postnatal care and also for surveillance and serosurvey studies. Since the viremia during ZIKV infection is fleeting, serological testing is highly valuable to inform diagnosis. However, current serology tests using whole virus antigens frequently suffer from cross reactivity issues, delays, and technical complexity, especially in low and middle income countries (LMICs) and endemic countries. Here, we describe an indirect ELISA to detect specific IgG antibodies using the ZIKV envelope domain III (EDIII) protein expressed in Drosophila S2 cells as an immunogen. Using a total of 367 clinical samples, we showed that the EDIII-ELISA was able to detect IgG antibodies against ZIKV with high sensitivity of 100.0% and specificity of 94.7% when compared to plaque reduction neutralization tests (PRNTs) as the gold standard and using 0.208 as the cut-off OD value. These results show the usefulness of the recombinant envelope domain III as an alternative to standard whole virus proteins for ZIKV diagnostics as it improves the sensitivity and specificity of IgG ELISA assay when used as an immunogen. This method should, therefore, be extended to serological diagnostic techniques for other members of the flavivirus genus and for use in IgM diagnostic testing. MDPI 2023-01-26 /pmc/articles/PMC9913938/ /pubmed/36766567 http://dx.doi.org/10.3390/diagnostics13030462 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ndiaye, Oumar
Diagne, Cheikh Tidiane
Abd El Wahed, Ahmed
Dia, Fatou
Dia, Moussa
Faye, Adama
Leal, Silvania Da Veiga
dos Santos, Menilita
de Lima Mendonça, Maria da Luz
da Silva Leite, Carolina Cardoso
Bouh Boye, Cheikh Saad
Bryant, Juliet E.
Desprès, Philippe
Faye, Ousmane
Sall, Amadou Alpha
Faye, Oumar
Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title_full Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title_fullStr Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title_full_unstemmed Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title_short Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
title_sort use of envelope domain iii protein for the detection of igg type antibodies specific to zika virus by indirect elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913938/
https://www.ncbi.nlm.nih.gov/pubmed/36766567
http://dx.doi.org/10.3390/diagnostics13030462
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