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Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella

Dried blood spots (DBSs) are an economical and convenient alternative to serum/plasma, which allow for the serological and molecular study of different pathogens. Sixty-four blood samples were collected by venipuncture and spotted onto Whatman™ 903 cards to evaluate the utility of DBSs and the effec...

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Autores principales: Rodríguez-Mateos, Mariano, Jaso, Javier, Martínez de Aguirre, Paula, Carlos, Silvia, Fernández-Ciriza, Leire, Holguín, África, Reina, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913955/
https://www.ncbi.nlm.nih.gov/pubmed/36766454
http://dx.doi.org/10.3390/diagnostics13030349
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author Rodríguez-Mateos, Mariano
Jaso, Javier
Martínez de Aguirre, Paula
Carlos, Silvia
Fernández-Ciriza, Leire
Holguín, África
Reina, Gabriel
author_facet Rodríguez-Mateos, Mariano
Jaso, Javier
Martínez de Aguirre, Paula
Carlos, Silvia
Fernández-Ciriza, Leire
Holguín, África
Reina, Gabriel
author_sort Rodríguez-Mateos, Mariano
collection PubMed
description Dried blood spots (DBSs) are an economical and convenient alternative to serum/plasma, which allow for the serological and molecular study of different pathogens. Sixty-four blood samples were collected by venipuncture and spotted onto Whatman™ 903 cards to evaluate the utility of DBSs and the effect of the storage temperature for 120 days after sample collection to carry out serological diagnosis. Mumps, measles and rubella IgG were investigated from DBSs and plasma using an automated chemiluminescent immunoassay. Using a calculated optimal cut-off value, the serological evaluation of mumps, measles and rubella using DBSs achieved high sensitivity (100%, 100% and 82.5%, respectively) and specificity (100%, 87.5% and 100%, respectively). The correlation observed between the plasma and the DBSs processed after sample collection was high (0.914–0.953) for all antibodies studied, both considering hematocrit before sample elution or not. For the different storage conditions, the correlation with plasma was high at 4 °C (0.889–0.925) and at −20 °C (0.878–0.951) but lower at room temperature (0.762–0.872). Measles IgG results were more affected than other markers when DBSs were stored at any temperature for 120 days. To summarize, hematocrit does not affect the processing of DBSs in the study of serological markers of mumps, measles and rubella. DBS stability for serological diagnosis of mumps and rubella is adequate when samples are stored at −20 °C or 4 °C, but not at room temperature, for a period of 4 months.
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spelling pubmed-99139552023-02-11 Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella Rodríguez-Mateos, Mariano Jaso, Javier Martínez de Aguirre, Paula Carlos, Silvia Fernández-Ciriza, Leire Holguín, África Reina, Gabriel Diagnostics (Basel) Article Dried blood spots (DBSs) are an economical and convenient alternative to serum/plasma, which allow for the serological and molecular study of different pathogens. Sixty-four blood samples were collected by venipuncture and spotted onto Whatman™ 903 cards to evaluate the utility of DBSs and the effect of the storage temperature for 120 days after sample collection to carry out serological diagnosis. Mumps, measles and rubella IgG were investigated from DBSs and plasma using an automated chemiluminescent immunoassay. Using a calculated optimal cut-off value, the serological evaluation of mumps, measles and rubella using DBSs achieved high sensitivity (100%, 100% and 82.5%, respectively) and specificity (100%, 87.5% and 100%, respectively). The correlation observed between the plasma and the DBSs processed after sample collection was high (0.914–0.953) for all antibodies studied, both considering hematocrit before sample elution or not. For the different storage conditions, the correlation with plasma was high at 4 °C (0.889–0.925) and at −20 °C (0.878–0.951) but lower at room temperature (0.762–0.872). Measles IgG results were more affected than other markers when DBSs were stored at any temperature for 120 days. To summarize, hematocrit does not affect the processing of DBSs in the study of serological markers of mumps, measles and rubella. DBS stability for serological diagnosis of mumps and rubella is adequate when samples are stored at −20 °C or 4 °C, but not at room temperature, for a period of 4 months. MDPI 2023-01-18 /pmc/articles/PMC9913955/ /pubmed/36766454 http://dx.doi.org/10.3390/diagnostics13030349 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Mateos, Mariano
Jaso, Javier
Martínez de Aguirre, Paula
Carlos, Silvia
Fernández-Ciriza, Leire
Holguín, África
Reina, Gabriel
Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title_full Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title_fullStr Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title_full_unstemmed Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title_short Effect of the Hematocrit and Storage Temperature of Dried Blood Samples in the Serological Study of Mumps, Measles and Rubella
title_sort effect of the hematocrit and storage temperature of dried blood samples in the serological study of mumps, measles and rubella
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913955/
https://www.ncbi.nlm.nih.gov/pubmed/36766454
http://dx.doi.org/10.3390/diagnostics13030349
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