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Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador
Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9914694/ https://www.ncbi.nlm.nih.gov/pubmed/36767267 http://dx.doi.org/10.3390/ijerph20031900 |
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author | Hernández-Alomía, Fernanda Bastidas-Caldes, Carlos Ballesteros, Isabel Tenea, Gabriela N. Jarrín-V., Pablo Molina, C. Alfonso Castillejo, Pablo |
author_facet | Hernández-Alomía, Fernanda Bastidas-Caldes, Carlos Ballesteros, Isabel Tenea, Gabriela N. Jarrín-V., Pablo Molina, C. Alfonso Castillejo, Pablo |
author_sort | Hernández-Alomía, Fernanda |
collection | PubMed |
description | Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through mec genes, which encode modified penicillin-binding proteins (PBP2). This study aimed to determine the presence and spread of β-lactam antibiotic resistance genes and the microbiome circulating in Quito’s Public Transport (QTP). A total of 29 station turnstiles were swabbed to extract the surface environmental DNA. PCRs were performed to detect the presence of 13 antibiotic resistance genes and to identify and to amplify 16S rDNA for barcoding, followed by clone analysis, Sanger sequencing, and BLAST search. ESBL genes bla(TEM-1) and bla(CTX-M-1) and MBL genes bla(OXA-181) and mecA were detected along QPT stations, blaTEM being the most widely spread. Two subvariants were found for bla(TEM-1), bla(CTX-M-1), and bla(OXA-181). Almost half of the circulating bacteria found at QPT stations were common human microbiota species, including those classified by the WHO as pathogens of critical and high-priority surveillance. β-lactam antibiotic resistance genes are prevalent throughout QPT. This is the first report of bla(OXA-181) in environmental samples in Ecuador. Moreover, we detected a new putative variant of this gene. Some commensal coagulase-negative bacteria may have a role as mecA resistance reservoirs. |
format | Online Article Text |
id | pubmed-9914694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99146942023-02-11 Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador Hernández-Alomía, Fernanda Bastidas-Caldes, Carlos Ballesteros, Isabel Tenea, Gabriela N. Jarrín-V., Pablo Molina, C. Alfonso Castillejo, Pablo Int J Environ Res Public Health Article Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through mec genes, which encode modified penicillin-binding proteins (PBP2). This study aimed to determine the presence and spread of β-lactam antibiotic resistance genes and the microbiome circulating in Quito’s Public Transport (QTP). A total of 29 station turnstiles were swabbed to extract the surface environmental DNA. PCRs were performed to detect the presence of 13 antibiotic resistance genes and to identify and to amplify 16S rDNA for barcoding, followed by clone analysis, Sanger sequencing, and BLAST search. ESBL genes bla(TEM-1) and bla(CTX-M-1) and MBL genes bla(OXA-181) and mecA were detected along QPT stations, blaTEM being the most widely spread. Two subvariants were found for bla(TEM-1), bla(CTX-M-1), and bla(OXA-181). Almost half of the circulating bacteria found at QPT stations were common human microbiota species, including those classified by the WHO as pathogens of critical and high-priority surveillance. β-lactam antibiotic resistance genes are prevalent throughout QPT. This is the first report of bla(OXA-181) in environmental samples in Ecuador. Moreover, we detected a new putative variant of this gene. Some commensal coagulase-negative bacteria may have a role as mecA resistance reservoirs. MDPI 2023-01-20 /pmc/articles/PMC9914694/ /pubmed/36767267 http://dx.doi.org/10.3390/ijerph20031900 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernández-Alomía, Fernanda Bastidas-Caldes, Carlos Ballesteros, Isabel Tenea, Gabriela N. Jarrín-V., Pablo Molina, C. Alfonso Castillejo, Pablo Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title | Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title_full | Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title_fullStr | Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title_full_unstemmed | Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title_short | Beta-Lactam Antibiotic Resistance Genes in the Microbiome of the Public Transport System of Quito, Ecuador |
title_sort | beta-lactam antibiotic resistance genes in the microbiome of the public transport system of quito, ecuador |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9914694/ https://www.ncbi.nlm.nih.gov/pubmed/36767267 http://dx.doi.org/10.3390/ijerph20031900 |
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