Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein

Here, we provide a protocol for the design, expression, purification, and functional studies of an engineered trimeric version of the receptor-binding domain (tRBD) of SARS-CoV-2 spike protein. We describe the use of tRBD to block SARS-CoV-2 spike pseudovirus and true virus binding to cellular angio...

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Autores principales: Liu, Angela Rose, Basavarajappa, Shrikanth C., Sarkar, Nandini, Bruchez, Anna, Ramakrishnan, Parameswaran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9915046/
https://www.ncbi.nlm.nih.gov/pubmed/36853707
http://dx.doi.org/10.1016/j.xpro.2023.102127
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author Liu, Angela Rose
Basavarajappa, Shrikanth C.
Sarkar, Nandini
Bruchez, Anna
Ramakrishnan, Parameswaran
author_facet Liu, Angela Rose
Basavarajappa, Shrikanth C.
Sarkar, Nandini
Bruchez, Anna
Ramakrishnan, Parameswaran
author_sort Liu, Angela Rose
collection PubMed
description Here, we provide a protocol for the design, expression, purification, and functional studies of an engineered trimeric version of the receptor-binding domain (tRBD) of SARS-CoV-2 spike protein. We describe the use of tRBD to block SARS-CoV-2 spike pseudovirus and true virus binding to cellular angiotensin converting enzyme-2 (ACE2), thereby blocking viral infection. This protocol is applicable to generate a trimeric version of any protein of interest. For complete details on the use and execution of this protocol, please refer to Basavarajappa et al. (2022).(1)
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spelling pubmed-99150462023-02-13 Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein Liu, Angela Rose Basavarajappa, Shrikanth C. Sarkar, Nandini Bruchez, Anna Ramakrishnan, Parameswaran STAR Protoc Protocol Here, we provide a protocol for the design, expression, purification, and functional studies of an engineered trimeric version of the receptor-binding domain (tRBD) of SARS-CoV-2 spike protein. We describe the use of tRBD to block SARS-CoV-2 spike pseudovirus and true virus binding to cellular angiotensin converting enzyme-2 (ACE2), thereby blocking viral infection. This protocol is applicable to generate a trimeric version of any protein of interest. For complete details on the use and execution of this protocol, please refer to Basavarajappa et al. (2022).(1) Elsevier 2023-02-10 /pmc/articles/PMC9915046/ /pubmed/36853707 http://dx.doi.org/10.1016/j.xpro.2023.102127 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Angela Rose
Basavarajappa, Shrikanth C.
Sarkar, Nandini
Bruchez, Anna
Ramakrishnan, Parameswaran
Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title_full Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title_fullStr Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title_full_unstemmed Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title_short Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
title_sort inhibition of sars-cov-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9915046/
https://www.ncbi.nlm.nih.gov/pubmed/36853707
http://dx.doi.org/10.1016/j.xpro.2023.102127
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