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Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research
Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and ce...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9915794/ https://www.ncbi.nlm.nih.gov/pubmed/36778283 http://dx.doi.org/10.21203/rs.3.rs-2505850/v1 |
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author | Shaw, Jane Alexandra Meiring, Maynard Allies, Devon Cruywagen, Lauren Fisher, Tarryn-Lee Kasavan, Kesheera Roos, Kelly Botha, Stefan Marc MacDonald, Candice Hiemstra, Andriëtte M. Simon, Donald van Rensburg, Ilana Flinn, Marika Shabangu, Ayanda Kuivaniemi, Helena Tromp, Gerard Malherbe, Stephanus T. Walzl, Gerhard du Plessis, Nelita |
author_facet | Shaw, Jane Alexandra Meiring, Maynard Allies, Devon Cruywagen, Lauren Fisher, Tarryn-Lee Kasavan, Kesheera Roos, Kelly Botha, Stefan Marc MacDonald, Candice Hiemstra, Andriëtte M. Simon, Donald van Rensburg, Ilana Flinn, Marika Shabangu, Ayanda Kuivaniemi, Helena Tromp, Gerard Malherbe, Stephanus T. Walzl, Gerhard du Plessis, Nelita |
author_sort | Shaw, Jane Alexandra |
collection | PubMed |
description | Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, and higher proportions of granulocytes, but this declined at the end of treatment. HIV infection was associated with lower cell yields and more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells. |
format | Online Article Text |
id | pubmed-9915794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-99157942023-02-11 Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research Shaw, Jane Alexandra Meiring, Maynard Allies, Devon Cruywagen, Lauren Fisher, Tarryn-Lee Kasavan, Kesheera Roos, Kelly Botha, Stefan Marc MacDonald, Candice Hiemstra, Andriëtte M. Simon, Donald van Rensburg, Ilana Flinn, Marika Shabangu, Ayanda Kuivaniemi, Helena Tromp, Gerard Malherbe, Stephanus T. Walzl, Gerhard du Plessis, Nelita Res Sq Article Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, and higher proportions of granulocytes, but this declined at the end of treatment. HIV infection was associated with lower cell yields and more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells. American Journal Experts 2023-02-02 /pmc/articles/PMC9915794/ /pubmed/36778283 http://dx.doi.org/10.21203/rs.3.rs-2505850/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. https://creativecommons.org/licenses/by/4.0/License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Article Shaw, Jane Alexandra Meiring, Maynard Allies, Devon Cruywagen, Lauren Fisher, Tarryn-Lee Kasavan, Kesheera Roos, Kelly Botha, Stefan Marc MacDonald, Candice Hiemstra, Andriëtte M. Simon, Donald van Rensburg, Ilana Flinn, Marika Shabangu, Ayanda Kuivaniemi, Helena Tromp, Gerard Malherbe, Stephanus T. Walzl, Gerhard du Plessis, Nelita Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title | Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title_full | Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title_fullStr | Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title_full_unstemmed | Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title_short | Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
title_sort | optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9915794/ https://www.ncbi.nlm.nih.gov/pubmed/36778283 http://dx.doi.org/10.21203/rs.3.rs-2505850/v1 |
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