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Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice

Dysfunctions of the ovaries and adrenal glands are both evidenced to cause aberrant adipose tissue (AT) remodeling and resultant metabolic disorders, but their distinct and common roles are poorly understood. In this study, through biochemical, histological and RNA-seq analyses, we comprehensively e...

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Autores principales: Chen, Weihao, Meng, Fengyan, Zeng, Xianyin, Cao, Xiaohan, Bu, Guixian, Du, Xiaogang, Yu, Guozhi, Kong, Fanli, Li, Yunkun, Gan, Tian, Han, Xingfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916485/
https://www.ncbi.nlm.nih.gov/pubmed/36768630
http://dx.doi.org/10.3390/ijms24032308
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author Chen, Weihao
Meng, Fengyan
Zeng, Xianyin
Cao, Xiaohan
Bu, Guixian
Du, Xiaogang
Yu, Guozhi
Kong, Fanli
Li, Yunkun
Gan, Tian
Han, Xingfa
author_facet Chen, Weihao
Meng, Fengyan
Zeng, Xianyin
Cao, Xiaohan
Bu, Guixian
Du, Xiaogang
Yu, Guozhi
Kong, Fanli
Li, Yunkun
Gan, Tian
Han, Xingfa
author_sort Chen, Weihao
collection PubMed
description Dysfunctions of the ovaries and adrenal glands are both evidenced to cause aberrant adipose tissue (AT) remodeling and resultant metabolic disorders, but their distinct and common roles are poorly understood. In this study, through biochemical, histological and RNA-seq analyses, we comprehensively explored the mechanisms underpinning subcutaneous (SAT) and visceral adipose tissue (VAT) remodeling, in response to ovariectomy (OVX) versus adrenalectomy (ADX) in female mice. OVX promoted adipocyte differentiation and fat accumulation in both SAT and VAT, by potentiating the Pparg signaling, while ADX universally prevented the cell proliferation and extracellular matrix organization in both SAT and VAT, likely by inactivating the Nr3c1 signaling, thus causing lipoatrophy in females. ADX, but not OVX, exerted great effects on the intrinsic difference between SAT and VAT. Specifically, ADX reversed a large cluster of genes differentially expressed between SAT and VAT, by activating 12 key transcription factors, and thereby caused senescent cell accumulation, massive B cell infiltration and the development of selective inflammatory response in SAT. Commonly, both OVX and ADX enhance circadian rhythmicity in VAT, and impair cell proliferation, neurogenesis, tissue morphogenesis, as well as extracellular matrix organization in SAT, thus causing dysfunction of adipose tissues and concomitant metabolic disorders.
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spelling pubmed-99164852023-02-11 Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice Chen, Weihao Meng, Fengyan Zeng, Xianyin Cao, Xiaohan Bu, Guixian Du, Xiaogang Yu, Guozhi Kong, Fanli Li, Yunkun Gan, Tian Han, Xingfa Int J Mol Sci Article Dysfunctions of the ovaries and adrenal glands are both evidenced to cause aberrant adipose tissue (AT) remodeling and resultant metabolic disorders, but their distinct and common roles are poorly understood. In this study, through biochemical, histological and RNA-seq analyses, we comprehensively explored the mechanisms underpinning subcutaneous (SAT) and visceral adipose tissue (VAT) remodeling, in response to ovariectomy (OVX) versus adrenalectomy (ADX) in female mice. OVX promoted adipocyte differentiation and fat accumulation in both SAT and VAT, by potentiating the Pparg signaling, while ADX universally prevented the cell proliferation and extracellular matrix organization in both SAT and VAT, likely by inactivating the Nr3c1 signaling, thus causing lipoatrophy in females. ADX, but not OVX, exerted great effects on the intrinsic difference between SAT and VAT. Specifically, ADX reversed a large cluster of genes differentially expressed between SAT and VAT, by activating 12 key transcription factors, and thereby caused senescent cell accumulation, massive B cell infiltration and the development of selective inflammatory response in SAT. Commonly, both OVX and ADX enhance circadian rhythmicity in VAT, and impair cell proliferation, neurogenesis, tissue morphogenesis, as well as extracellular matrix organization in SAT, thus causing dysfunction of adipose tissues and concomitant metabolic disorders. MDPI 2023-01-24 /pmc/articles/PMC9916485/ /pubmed/36768630 http://dx.doi.org/10.3390/ijms24032308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Weihao
Meng, Fengyan
Zeng, Xianyin
Cao, Xiaohan
Bu, Guixian
Du, Xiaogang
Yu, Guozhi
Kong, Fanli
Li, Yunkun
Gan, Tian
Han, Xingfa
Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title_full Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title_fullStr Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title_full_unstemmed Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title_short Mechanic Insight into the Distinct and Common Roles of Ovariectomy Versus Adrenalectomy on Adipose Tissue Remodeling in Female Mice
title_sort mechanic insight into the distinct and common roles of ovariectomy versus adrenalectomy on adipose tissue remodeling in female mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916485/
https://www.ncbi.nlm.nih.gov/pubmed/36768630
http://dx.doi.org/10.3390/ijms24032308
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