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Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants

Gnotobiotic (GN) animals with defined microbiota allow us to study host–microbiota and microbiota–microbiota interferences. Preterm germ-free (GF) piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to ameliorate/prevent the consequences of infection with...

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Autores principales: Splichal, Igor, Donovan, Sharon M., Kindlova, Zdislava, Stranak, Zbynek, Neuzil Bunesova, Vera, Sinkora, Marek, Polakova, Katerina, Valaskova, Barbora, Splichalova, Alla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916534/
https://www.ncbi.nlm.nih.gov/pubmed/36768650
http://dx.doi.org/10.3390/ijms24032329
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author Splichal, Igor
Donovan, Sharon M.
Kindlova, Zdislava
Stranak, Zbynek
Neuzil Bunesova, Vera
Sinkora, Marek
Polakova, Katerina
Valaskova, Barbora
Splichalova, Alla
author_facet Splichal, Igor
Donovan, Sharon M.
Kindlova, Zdislava
Stranak, Zbynek
Neuzil Bunesova, Vera
Sinkora, Marek
Polakova, Katerina
Valaskova, Barbora
Splichalova, Alla
author_sort Splichal, Igor
collection PubMed
description Gnotobiotic (GN) animals with defined microbiota allow us to study host–microbiota and microbiota–microbiota interferences. Preterm germ-free (GF) piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to ameliorate/prevent the consequences of infection with the Salmonella Typhimurium strain LT2 (LT2). Goblet cell density; expression of Toll-like receptors (TLRs) 2, 4, and 9; high mobility group box 1 (HMGB1); interleukin (IL)-6; and IL-12/23p40 were analyzed to evaluate the possible modulatory effect of BB12. BB12 prevented an LT2-induced decrease of goblet cell density in the colon. TLRs signaling modified by LT2 was not influenced by the previous association with BB12. The expression of HMGB1, IL-6, and IL12/23p40 in the jejunum, ileum, and colon and their levels in plasma were all decreased by BB12, but these changes were not statistically significant. In the colon, differences in HMGB1 distribution between the GF and LT2 piglet groups were observed. In conclusion, the mono-association of GF piglets with BB12 prior to LT2 infection partially ameliorated the inflammatory response to LT2 infection.
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spelling pubmed-99165342023-02-11 Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants Splichal, Igor Donovan, Sharon M. Kindlova, Zdislava Stranak, Zbynek Neuzil Bunesova, Vera Sinkora, Marek Polakova, Katerina Valaskova, Barbora Splichalova, Alla Int J Mol Sci Article Gnotobiotic (GN) animals with defined microbiota allow us to study host–microbiota and microbiota–microbiota interferences. Preterm germ-free (GF) piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to ameliorate/prevent the consequences of infection with the Salmonella Typhimurium strain LT2 (LT2). Goblet cell density; expression of Toll-like receptors (TLRs) 2, 4, and 9; high mobility group box 1 (HMGB1); interleukin (IL)-6; and IL-12/23p40 were analyzed to evaluate the possible modulatory effect of BB12. BB12 prevented an LT2-induced decrease of goblet cell density in the colon. TLRs signaling modified by LT2 was not influenced by the previous association with BB12. The expression of HMGB1, IL-6, and IL12/23p40 in the jejunum, ileum, and colon and their levels in plasma were all decreased by BB12, but these changes were not statistically significant. In the colon, differences in HMGB1 distribution between the GF and LT2 piglet groups were observed. In conclusion, the mono-association of GF piglets with BB12 prior to LT2 infection partially ameliorated the inflammatory response to LT2 infection. MDPI 2023-01-24 /pmc/articles/PMC9916534/ /pubmed/36768650 http://dx.doi.org/10.3390/ijms24032329 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Splichal, Igor
Donovan, Sharon M.
Kindlova, Zdislava
Stranak, Zbynek
Neuzil Bunesova, Vera
Sinkora, Marek
Polakova, Katerina
Valaskova, Barbora
Splichalova, Alla
Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title_full Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title_fullStr Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title_full_unstemmed Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title_short Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants
title_sort release of hmgb1 and toll-like receptors 2, 4, and 9 signaling are modulated by bifidobacterium animalis subsp. lactis bb-12 and salmonella typhimurium in a gnotobiotic piglet model of preterm infants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916534/
https://www.ncbi.nlm.nih.gov/pubmed/36768650
http://dx.doi.org/10.3390/ijms24032329
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