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Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology
The role of metallic nano- and microparticles in the development of inflammation has not yet been investigated. Soft tissue biopsy specimens of the bone bed taken during surgical revisions, as well as supernatants obtained from the surface of the orthopedic structures and dental implants (control),...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916569/ https://www.ncbi.nlm.nih.gov/pubmed/36768589 http://dx.doi.org/10.3390/ijms24032267 |
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author | Labis, Varvara Bazikyan, Ernest Demin, Denis Dyachkova, Irina Zolotov, Denis Volkov, Alexey Asadchikov, Victor Zhigalina, Olga Khmelenin, Dmitry Kuptsova, Daria Petrichuk, Svetlana Semikina, Elena Sizova, Svetlana Oleinikov, Vladimir Khaidukov, Sergey Kozlov, Ivan |
author_facet | Labis, Varvara Bazikyan, Ernest Demin, Denis Dyachkova, Irina Zolotov, Denis Volkov, Alexey Asadchikov, Victor Zhigalina, Olga Khmelenin, Dmitry Kuptsova, Daria Petrichuk, Svetlana Semikina, Elena Sizova, Svetlana Oleinikov, Vladimir Khaidukov, Sergey Kozlov, Ivan |
author_sort | Labis, Varvara |
collection | PubMed |
description | The role of metallic nano- and microparticles in the development of inflammation has not yet been investigated. Soft tissue biopsy specimens of the bone bed taken during surgical revisions, as well as supernatants obtained from the surface of the orthopedic structures and dental implants (control), were examined. Investigations were performed using X-ray microtomography, X-ray fluorescence analysis, and scanning electron microscopy. Histological studies of the bone bed tissues were performed. Nanoscale and microscale metallic particles were identified as participants in the inflammatory process in tissues. Supernatants containing nanoscale particles were obtained from the surfaces of 20 units of new dental implants. Early and late apoptosis and necrosis of immunocompetent cells after co-culture and induction by lipopolysaccharide and human venous blood serum were studied in an experiment with staging on the THP-1 (human monocytic) cell line using visualizing cytometry. As a result, it was found that nano- and microparticles emitted from the surface of the oxide layer of medical devices impregnated soft tissue biopsy specimens. By using different methods to analyze the cell–molecule interactions of nano- and microparticles both from a clinical perspective and an experimental research perspective, the possibility of forming a chronic immunopathological endogenous inflammatory process with an autoimmune component in the tissues was revealed. |
format | Online Article Text |
id | pubmed-9916569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99165692023-02-11 Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology Labis, Varvara Bazikyan, Ernest Demin, Denis Dyachkova, Irina Zolotov, Denis Volkov, Alexey Asadchikov, Victor Zhigalina, Olga Khmelenin, Dmitry Kuptsova, Daria Petrichuk, Svetlana Semikina, Elena Sizova, Svetlana Oleinikov, Vladimir Khaidukov, Sergey Kozlov, Ivan Int J Mol Sci Article The role of metallic nano- and microparticles in the development of inflammation has not yet been investigated. Soft tissue biopsy specimens of the bone bed taken during surgical revisions, as well as supernatants obtained from the surface of the orthopedic structures and dental implants (control), were examined. Investigations were performed using X-ray microtomography, X-ray fluorescence analysis, and scanning electron microscopy. Histological studies of the bone bed tissues were performed. Nanoscale and microscale metallic particles were identified as participants in the inflammatory process in tissues. Supernatants containing nanoscale particles were obtained from the surfaces of 20 units of new dental implants. Early and late apoptosis and necrosis of immunocompetent cells after co-culture and induction by lipopolysaccharide and human venous blood serum were studied in an experiment with staging on the THP-1 (human monocytic) cell line using visualizing cytometry. As a result, it was found that nano- and microparticles emitted from the surface of the oxide layer of medical devices impregnated soft tissue biopsy specimens. By using different methods to analyze the cell–molecule interactions of nano- and microparticles both from a clinical perspective and an experimental research perspective, the possibility of forming a chronic immunopathological endogenous inflammatory process with an autoimmune component in the tissues was revealed. MDPI 2023-01-23 /pmc/articles/PMC9916569/ /pubmed/36768589 http://dx.doi.org/10.3390/ijms24032267 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Labis, Varvara Bazikyan, Ernest Demin, Denis Dyachkova, Irina Zolotov, Denis Volkov, Alexey Asadchikov, Victor Zhigalina, Olga Khmelenin, Dmitry Kuptsova, Daria Petrichuk, Svetlana Semikina, Elena Sizova, Svetlana Oleinikov, Vladimir Khaidukov, Sergey Kozlov, Ivan Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title | Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title_full | Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title_fullStr | Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title_full_unstemmed | Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title_short | Cell-Molecular Interactions of Nano- and Microparticles in Dental Implantology |
title_sort | cell-molecular interactions of nano- and microparticles in dental implantology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916569/ https://www.ncbi.nlm.nih.gov/pubmed/36768589 http://dx.doi.org/10.3390/ijms24032267 |
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