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Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis
Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/0...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916614/ https://www.ncbi.nlm.nih.gov/pubmed/36768700 http://dx.doi.org/10.3390/ijms24032376 |
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author | Desheva, Yulia Petkova, Nadezhda Losev, Igor Guzhov, Dmitry Go, Alexey Chao, Yu-Chan Tsai, Chih-Hsuan |
author_facet | Desheva, Yulia Petkova, Nadezhda Losev, Igor Guzhov, Dmitry Go, Alexey Chao, Yu-Chan Tsai, Chih-Hsuan |
author_sort | Desheva, Yulia |
collection | PubMed |
description | Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/07/2009 (H1N1)pdm09 strain. We evaluated the level of NA-inhibiting (NI) antibodies in the paired blood sera of influenza patients by means of an enzyme-linked lectin assay (ELLA) using the influenza virus or N1-Bac. Additionally, we evaluated the level of NA antibodies by means of the enzyme-linked immunosorbent assay (ELISA) with an N1-expressing Sf21 culture. We detected a strong correlation between our results from using the influenza virus and NA-Bac pseudoviruses to detect NI antibodies and a medium-strong correlation between NI antibodies and NA antibodies determined by an N1-cell ELISA, indicating that baculovirus-based platforms can be successfully used to evaluate NI or NA antibodies. Furthermore, animal experiments showed that immunization with N1-Bac protected against infection with a drift variant of the A/H1N1pdm09 influenza virus. Our results demonstrate that recombinant baculovirus can be an effective influenza pseudotype to evaluate influenza serologic immunity and protect against influenza virus infection. |
format | Online Article Text |
id | pubmed-9916614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99166142023-02-11 Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis Desheva, Yulia Petkova, Nadezhda Losev, Igor Guzhov, Dmitry Go, Alexey Chao, Yu-Chan Tsai, Chih-Hsuan Int J Mol Sci Article Neuraminidase (NA)-based immunity to influenza can be useful for protecting against novel antigenic variants. To develop safe and effective tools to assess NA-based immunity, we generated a baculovirus-based pseudotyped virus, N1-Bac, that expresses the full-length NA of the influenza A/California/07/2009 (H1N1)pdm09 strain. We evaluated the level of NA-inhibiting (NI) antibodies in the paired blood sera of influenza patients by means of an enzyme-linked lectin assay (ELLA) using the influenza virus or N1-Bac. Additionally, we evaluated the level of NA antibodies by means of the enzyme-linked immunosorbent assay (ELISA) with an N1-expressing Sf21 culture. We detected a strong correlation between our results from using the influenza virus and NA-Bac pseudoviruses to detect NI antibodies and a medium-strong correlation between NI antibodies and NA antibodies determined by an N1-cell ELISA, indicating that baculovirus-based platforms can be successfully used to evaluate NI or NA antibodies. Furthermore, animal experiments showed that immunization with N1-Bac protected against infection with a drift variant of the A/H1N1pdm09 influenza virus. Our results demonstrate that recombinant baculovirus can be an effective influenza pseudotype to evaluate influenza serologic immunity and protect against influenza virus infection. MDPI 2023-01-25 /pmc/articles/PMC9916614/ /pubmed/36768700 http://dx.doi.org/10.3390/ijms24032376 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Desheva, Yulia Petkova, Nadezhda Losev, Igor Guzhov, Dmitry Go, Alexey Chao, Yu-Chan Tsai, Chih-Hsuan Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title | Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title_full | Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title_fullStr | Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title_full_unstemmed | Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title_short | Establishment of a Pseudovirus Platform for Neuraminidase Inhibiting Antibody Analysis |
title_sort | establishment of a pseudovirus platform for neuraminidase inhibiting antibody analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916614/ https://www.ncbi.nlm.nih.gov/pubmed/36768700 http://dx.doi.org/10.3390/ijms24032376 |
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