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Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye

Cyclosporine A (CsA) as an eye drop is an effective treatment for dry eye. However, it has potential side effects and a short ocular residence time. To overcome these obstacles, we developed a cellulose acetate phthalate-based pH-responsive contact lens (CL) loaded with CsA (CsA-CL). The CsA was con...

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Autores principales: Kim, Jonghwa, Mondal, Himangsu, Jin, Rujun, Yoon, Hyeon Jeong, Kim, Ho-Joong, Jee, Jun-Pil, Yoon, Kyung Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916649/
https://www.ncbi.nlm.nih.gov/pubmed/36768682
http://dx.doi.org/10.3390/ijms24032361
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author Kim, Jonghwa
Mondal, Himangsu
Jin, Rujun
Yoon, Hyeon Jeong
Kim, Ho-Joong
Jee, Jun-Pil
Yoon, Kyung Chul
author_facet Kim, Jonghwa
Mondal, Himangsu
Jin, Rujun
Yoon, Hyeon Jeong
Kim, Ho-Joong
Jee, Jun-Pil
Yoon, Kyung Chul
author_sort Kim, Jonghwa
collection PubMed
description Cyclosporine A (CsA) as an eye drop is an effective treatment for dry eye. However, it has potential side effects and a short ocular residence time. To overcome these obstacles, we developed a cellulose acetate phthalate-based pH-responsive contact lens (CL) loaded with CsA (CsA-CL). The CsA was continuously released from the CsA-CL at physiological conditions (37 °C, pH 7.4) without an initial burst. CsA was well-contained in the selected storage condition (4 °C, pH 5.4) for as long as 90 days. In safety assays, cytotoxicity, ocular irritation, visible light transmittance, and oxygen permeability were in a normal range. CsA concentrations in the conjunctiva, cornea, and lens increased over time until 12 h. When comparing the therapeutic efficacy between the normal control, experimental dry eye (EDE), and treatment groups (CsA eye drop, naïve CL, and CsA-CL groups), the tear volume, TBUT, corneal fluorescein staining at 7 and 14 days, conjunctival goblet cell density, and corneal apoptotic cell counts at 14 days improved in all treatment groups compared to EDE, with a significantly better result in the CsA-CL group compared with other groups (all p < 0.05). The CsA-CL could be an effective, stable, and safe option for inflammatory dry eye.
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spelling pubmed-99166492023-02-11 Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye Kim, Jonghwa Mondal, Himangsu Jin, Rujun Yoon, Hyeon Jeong Kim, Ho-Joong Jee, Jun-Pil Yoon, Kyung Chul Int J Mol Sci Article Cyclosporine A (CsA) as an eye drop is an effective treatment for dry eye. However, it has potential side effects and a short ocular residence time. To overcome these obstacles, we developed a cellulose acetate phthalate-based pH-responsive contact lens (CL) loaded with CsA (CsA-CL). The CsA was continuously released from the CsA-CL at physiological conditions (37 °C, pH 7.4) without an initial burst. CsA was well-contained in the selected storage condition (4 °C, pH 5.4) for as long as 90 days. In safety assays, cytotoxicity, ocular irritation, visible light transmittance, and oxygen permeability were in a normal range. CsA concentrations in the conjunctiva, cornea, and lens increased over time until 12 h. When comparing the therapeutic efficacy between the normal control, experimental dry eye (EDE), and treatment groups (CsA eye drop, naïve CL, and CsA-CL groups), the tear volume, TBUT, corneal fluorescein staining at 7 and 14 days, conjunctival goblet cell density, and corneal apoptotic cell counts at 14 days improved in all treatment groups compared to EDE, with a significantly better result in the CsA-CL group compared with other groups (all p < 0.05). The CsA-CL could be an effective, stable, and safe option for inflammatory dry eye. MDPI 2023-01-25 /pmc/articles/PMC9916649/ /pubmed/36768682 http://dx.doi.org/10.3390/ijms24032361 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jonghwa
Mondal, Himangsu
Jin, Rujun
Yoon, Hyeon Jeong
Kim, Ho-Joong
Jee, Jun-Pil
Yoon, Kyung Chul
Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title_full Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title_fullStr Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title_full_unstemmed Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title_short Cellulose Acetate Phthalate-Based pH-Responsive Cyclosporine A-Loaded Contact Lens for the Treatment of Dry Eye
title_sort cellulose acetate phthalate-based ph-responsive cyclosporine a-loaded contact lens for the treatment of dry eye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916649/
https://www.ncbi.nlm.nih.gov/pubmed/36768682
http://dx.doi.org/10.3390/ijms24032361
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