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Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications

Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypo...

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Autores principales: Costa, Eduardo M., Oliveira, Ana Sofia, Silva, Sara, Ribeiro, Alessandra B., Pereira, Carla F., Ferreira, Carlos, Casanova, Francisca, Pereira, Joana O., Freixo, Ricardo, Pintado, Manuela E., Carvalho, Ana Paula, Ramos, Óscar L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916692/
https://www.ncbi.nlm.nih.gov/pubmed/36768574
http://dx.doi.org/10.3390/ijms24032253
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author Costa, Eduardo M.
Oliveira, Ana Sofia
Silva, Sara
Ribeiro, Alessandra B.
Pereira, Carla F.
Ferreira, Carlos
Casanova, Francisca
Pereira, Joana O.
Freixo, Ricardo
Pintado, Manuela E.
Carvalho, Ana Paula
Ramos, Óscar L.
author_facet Costa, Eduardo M.
Oliveira, Ana Sofia
Silva, Sara
Ribeiro, Alessandra B.
Pereira, Carla F.
Ferreira, Carlos
Casanova, Francisca
Pereira, Joana O.
Freixo, Ricardo
Pintado, Manuela E.
Carvalho, Ana Paula
Ramos, Óscar L.
author_sort Costa, Eduardo M.
collection PubMed
description Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypoallergenic while simultaneously being able to exert various effects upon the epidermis modulating immune response and targeting skin metabolites, such as collagen production. As the potential of spent yeast’s peptides has been mainly explored for food-related applications, this work sought to understand if peptide fractions previously extracted from fermentation engineered spent yeast (Saccharomyces cerevisiae) waste streams possess biological potential for skin-related applications. To that end, cytotoxic effects on HaCat and HDFa cells and whether they were capable of exerting a positive effect upon the production of skin metabolites relevant for skin health, such as collagen, hyaluronic acid, fibronectin and elastin, were evaluated. The results showed that the peptide fractions assayed were not cytotoxic up to the highest concentration tested (500 µg/mL) for both cell lines tested. Furthermore, all peptide fractions showed a capacity to modulate the various target metabolites production with an overall positive effect being observed for the four fractions over the six selected targets (pro-collagen IαI, hyaluronic acid, fibronectin, cytokeratin-14, elastin, and aquaporin-9). Concerning the evaluated fractions, the overall best performance (Gpep > 1 kDa) was of an average promotion of 41.25% over the six metabolites and two cell lines assessed at a concentration of 100 µg/mL. These results showed that the peptide fractions assayed in this work have potential for future applications in skin-related products at relatively low concentrations, thus providing an alternative solution for one of the fermentation industry’s waste streams and creating a novel and highly valuable bioactive ingredient with encompassing activity to be applied in future skin care formulations.
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spelling pubmed-99166922023-02-11 Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications Costa, Eduardo M. Oliveira, Ana Sofia Silva, Sara Ribeiro, Alessandra B. Pereira, Carla F. Ferreira, Carlos Casanova, Francisca Pereira, Joana O. Freixo, Ricardo Pintado, Manuela E. Carvalho, Ana Paula Ramos, Óscar L. Int J Mol Sci Article Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypoallergenic while simultaneously being able to exert various effects upon the epidermis modulating immune response and targeting skin metabolites, such as collagen production. As the potential of spent yeast’s peptides has been mainly explored for food-related applications, this work sought to understand if peptide fractions previously extracted from fermentation engineered spent yeast (Saccharomyces cerevisiae) waste streams possess biological potential for skin-related applications. To that end, cytotoxic effects on HaCat and HDFa cells and whether they were capable of exerting a positive effect upon the production of skin metabolites relevant for skin health, such as collagen, hyaluronic acid, fibronectin and elastin, were evaluated. The results showed that the peptide fractions assayed were not cytotoxic up to the highest concentration tested (500 µg/mL) for both cell lines tested. Furthermore, all peptide fractions showed a capacity to modulate the various target metabolites production with an overall positive effect being observed for the four fractions over the six selected targets (pro-collagen IαI, hyaluronic acid, fibronectin, cytokeratin-14, elastin, and aquaporin-9). Concerning the evaluated fractions, the overall best performance (Gpep > 1 kDa) was of an average promotion of 41.25% over the six metabolites and two cell lines assessed at a concentration of 100 µg/mL. These results showed that the peptide fractions assayed in this work have potential for future applications in skin-related products at relatively low concentrations, thus providing an alternative solution for one of the fermentation industry’s waste streams and creating a novel and highly valuable bioactive ingredient with encompassing activity to be applied in future skin care formulations. MDPI 2023-01-23 /pmc/articles/PMC9916692/ /pubmed/36768574 http://dx.doi.org/10.3390/ijms24032253 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Costa, Eduardo M.
Oliveira, Ana Sofia
Silva, Sara
Ribeiro, Alessandra B.
Pereira, Carla F.
Ferreira, Carlos
Casanova, Francisca
Pereira, Joana O.
Freixo, Ricardo
Pintado, Manuela E.
Carvalho, Ana Paula
Ramos, Óscar L.
Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title_full Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title_fullStr Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title_full_unstemmed Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title_short Spent Yeast Waste Streams as a Sustainable Source of Bioactive Peptides for Skin Applications
title_sort spent yeast waste streams as a sustainable source of bioactive peptides for skin applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916692/
https://www.ncbi.nlm.nih.gov/pubmed/36768574
http://dx.doi.org/10.3390/ijms24032253
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