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Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization

Circulating tumor cells (CTCs), and particularly circulating cancer stem cells (cCSC), are prognostic biomarkers for different malignancies and may be detected using liquid biopsies. The ex vivo culture of cCSCs would provide valuable information regarding biological aggressiveness and would allow m...

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Autores principales: Espejo-Cruz, María L., González-Rubio, Sandra, Espejo, Juan J., Zamora-Olaya, Javier M., Alejandre-Altamirano, Rafael M., Prieto-Torre, María, Linares, Clara I., Guerrero-Misas, Marta, Barrera-Baena, Pilar, Poyato-González, Antonio, Sánchez-Frías, Marina, Ayllón, María D., Rodríguez-Perálvarez, Manuel L., de la Mata, Manuel, Ferrín, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916725/
https://www.ncbi.nlm.nih.gov/pubmed/36768881
http://dx.doi.org/10.3390/ijms24032558
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author Espejo-Cruz, María L.
González-Rubio, Sandra
Espejo, Juan J.
Zamora-Olaya, Javier M.
Alejandre-Altamirano, Rafael M.
Prieto-Torre, María
Linares, Clara I.
Guerrero-Misas, Marta
Barrera-Baena, Pilar
Poyato-González, Antonio
Sánchez-Frías, Marina
Ayllón, María D.
Rodríguez-Perálvarez, Manuel L.
de la Mata, Manuel
Ferrín, Gustavo
author_facet Espejo-Cruz, María L.
González-Rubio, Sandra
Espejo, Juan J.
Zamora-Olaya, Javier M.
Alejandre-Altamirano, Rafael M.
Prieto-Torre, María
Linares, Clara I.
Guerrero-Misas, Marta
Barrera-Baena, Pilar
Poyato-González, Antonio
Sánchez-Frías, Marina
Ayllón, María D.
Rodríguez-Perálvarez, Manuel L.
de la Mata, Manuel
Ferrín, Gustavo
author_sort Espejo-Cruz, María L.
collection PubMed
description Circulating tumor cells (CTCs), and particularly circulating cancer stem cells (cCSC), are prognostic biomarkers for different malignancies and may be detected using liquid biopsies. The ex vivo culture of cCSCs would provide valuable information regarding biological aggressiveness and would allow monitoring the adaptive changes acquired by the tumor in real time. In this prospective pilot study, we analyzed the presence of EpCAM(+) CTCs using the IsoFlux system in the peripheral blood of 37 patients with hepatocellular carcinoma undergoing transarterial chemoembolization (TACE). The average patient age was 63.5 ± 7.9 years and 91.9% of the patients were men. All patients had detectable CTCs at baseline and 20 patients (54.1%) showed CTC aggregates or clusters in their peripheral blood. The increased total tumor diameter (OR: 2.5 (95% CI: 1.3–4.8), p = 0.006) and the absence of clusters of CTCs at baseline (OR: 0.2 (95% CI: 0.0–1.0), p = 0.049) were independent predictors of a diminished response to TACE. Culture of cCSC was successful in five out of thirty-three patients, mostly using negative enrichment of CD45(−) cells, ultra-low adherence, high glucose, and a short period of hypoxia followed by normoxia. In conclusion, the identification of clusters of CTCs before TACE and the implementation of standardized approaches for cCSC culture could aid to predict outcomes and to define the optimal adjuvant therapeutic strategy for a true personalized medicine in hepatocellular carcinoma.
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spelling pubmed-99167252023-02-11 Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization Espejo-Cruz, María L. González-Rubio, Sandra Espejo, Juan J. Zamora-Olaya, Javier M. Alejandre-Altamirano, Rafael M. Prieto-Torre, María Linares, Clara I. Guerrero-Misas, Marta Barrera-Baena, Pilar Poyato-González, Antonio Sánchez-Frías, Marina Ayllón, María D. Rodríguez-Perálvarez, Manuel L. de la Mata, Manuel Ferrín, Gustavo Int J Mol Sci Article Circulating tumor cells (CTCs), and particularly circulating cancer stem cells (cCSC), are prognostic biomarkers for different malignancies and may be detected using liquid biopsies. The ex vivo culture of cCSCs would provide valuable information regarding biological aggressiveness and would allow monitoring the adaptive changes acquired by the tumor in real time. In this prospective pilot study, we analyzed the presence of EpCAM(+) CTCs using the IsoFlux system in the peripheral blood of 37 patients with hepatocellular carcinoma undergoing transarterial chemoembolization (TACE). The average patient age was 63.5 ± 7.9 years and 91.9% of the patients were men. All patients had detectable CTCs at baseline and 20 patients (54.1%) showed CTC aggregates or clusters in their peripheral blood. The increased total tumor diameter (OR: 2.5 (95% CI: 1.3–4.8), p = 0.006) and the absence of clusters of CTCs at baseline (OR: 0.2 (95% CI: 0.0–1.0), p = 0.049) were independent predictors of a diminished response to TACE. Culture of cCSC was successful in five out of thirty-three patients, mostly using negative enrichment of CD45(−) cells, ultra-low adherence, high glucose, and a short period of hypoxia followed by normoxia. In conclusion, the identification of clusters of CTCs before TACE and the implementation of standardized approaches for cCSC culture could aid to predict outcomes and to define the optimal adjuvant therapeutic strategy for a true personalized medicine in hepatocellular carcinoma. MDPI 2023-01-29 /pmc/articles/PMC9916725/ /pubmed/36768881 http://dx.doi.org/10.3390/ijms24032558 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Espejo-Cruz, María L.
González-Rubio, Sandra
Espejo, Juan J.
Zamora-Olaya, Javier M.
Alejandre-Altamirano, Rafael M.
Prieto-Torre, María
Linares, Clara I.
Guerrero-Misas, Marta
Barrera-Baena, Pilar
Poyato-González, Antonio
Sánchez-Frías, Marina
Ayllón, María D.
Rodríguez-Perálvarez, Manuel L.
de la Mata, Manuel
Ferrín, Gustavo
Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title_full Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title_fullStr Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title_full_unstemmed Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title_short Enumeration and Characterization of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization
title_sort enumeration and characterization of circulating tumor cells in patients with hepatocellular carcinoma undergoing transarterial chemoembolization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916725/
https://www.ncbi.nlm.nih.gov/pubmed/36768881
http://dx.doi.org/10.3390/ijms24032558
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