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Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)

Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP) are low-risk thyroid lesions most often characterised by RAS-type mutations. The histological diagnosis may be challenging, and even immunohistochemistry and molecular approaches have not yet provided conclusive so...

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Autores principales: Piga, Isabella, L’Imperio, Vincenzo, Principi, Lucrezia, Bellevicine, Claudio, Fusco, Nicola, Maffini, Fausto, Venetis, Konstantinos, Ivanova, Mariia, Seminati, Davide, Casati, Gabriele, Pagani, Lisa, Galimberti, Stefania, Capitoli, Giulia, Garancini, Mattia, Gatti, Andrea-Valer, Magni, Fulvio, Pagni, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916790/
https://www.ncbi.nlm.nih.gov/pubmed/36768889
http://dx.doi.org/10.3390/ijms24032567
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author Piga, Isabella
L’Imperio, Vincenzo
Principi, Lucrezia
Bellevicine, Claudio
Fusco, Nicola
Maffini, Fausto
Venetis, Konstantinos
Ivanova, Mariia
Seminati, Davide
Casati, Gabriele
Pagani, Lisa
Galimberti, Stefania
Capitoli, Giulia
Garancini, Mattia
Gatti, Andrea-Valer
Magni, Fulvio
Pagni, Fabio
author_facet Piga, Isabella
L’Imperio, Vincenzo
Principi, Lucrezia
Bellevicine, Claudio
Fusco, Nicola
Maffini, Fausto
Venetis, Konstantinos
Ivanova, Mariia
Seminati, Davide
Casati, Gabriele
Pagani, Lisa
Galimberti, Stefania
Capitoli, Giulia
Garancini, Mattia
Gatti, Andrea-Valer
Magni, Fulvio
Pagni, Fabio
author_sort Piga, Isabella
collection PubMed
description Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP) are low-risk thyroid lesions most often characterised by RAS-type mutations. The histological diagnosis may be challenging, and even immunohistochemistry and molecular approaches have not yet provided conclusive solutions. This study characterises a set of NIFTPs by Matrix-Assisted Laser Desorption/Ionisation (MALDI)–Mass Spectrometry Imaging (MSI) to highlight the proteomic signatures capable of overcoming histological challenges. Archived formalin-fixed paraffin-embedded samples from 10 NIFTPs (n = 6 RAS-mutated and n = 4 RAS-wild type) were trypsin-digested and analysed by MALDI–MSI, comparing their profiles to normal tissue and synchronous benign nodules. This allowed the definition of a four-peptide signature able to distinguish RAS-mutant from wild-type cases, the latter showing proteomic similarities to hyperplastic nodules. Moreover, among the differentially expressed signals, Peptidylprolyl Isomerase A (PPIA, 1505.8 m/z), which has already demonstrated a role in the development of cancer, was found overexpressed in NIFTP RAS-mutated nodules compared to wild-type lesions. These results underlined that high-throughput proteomic approaches may add a further level of biological comprehension for NIFTPs. In the future, thanks to the powerful single-cell detail achieved by new instruments, the complementary NGS–MALDI imaging sequence might be the correct methodological approach to confirm that the current NIFTP definition encompasses heterogeneous lesions that must be further characterised.
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spelling pubmed-99167902023-02-11 Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs) Piga, Isabella L’Imperio, Vincenzo Principi, Lucrezia Bellevicine, Claudio Fusco, Nicola Maffini, Fausto Venetis, Konstantinos Ivanova, Mariia Seminati, Davide Casati, Gabriele Pagani, Lisa Galimberti, Stefania Capitoli, Giulia Garancini, Mattia Gatti, Andrea-Valer Magni, Fulvio Pagni, Fabio Int J Mol Sci Article Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP) are low-risk thyroid lesions most often characterised by RAS-type mutations. The histological diagnosis may be challenging, and even immunohistochemistry and molecular approaches have not yet provided conclusive solutions. This study characterises a set of NIFTPs by Matrix-Assisted Laser Desorption/Ionisation (MALDI)–Mass Spectrometry Imaging (MSI) to highlight the proteomic signatures capable of overcoming histological challenges. Archived formalin-fixed paraffin-embedded samples from 10 NIFTPs (n = 6 RAS-mutated and n = 4 RAS-wild type) were trypsin-digested and analysed by MALDI–MSI, comparing their profiles to normal tissue and synchronous benign nodules. This allowed the definition of a four-peptide signature able to distinguish RAS-mutant from wild-type cases, the latter showing proteomic similarities to hyperplastic nodules. Moreover, among the differentially expressed signals, Peptidylprolyl Isomerase A (PPIA, 1505.8 m/z), which has already demonstrated a role in the development of cancer, was found overexpressed in NIFTP RAS-mutated nodules compared to wild-type lesions. These results underlined that high-throughput proteomic approaches may add a further level of biological comprehension for NIFTPs. In the future, thanks to the powerful single-cell detail achieved by new instruments, the complementary NGS–MALDI imaging sequence might be the correct methodological approach to confirm that the current NIFTP definition encompasses heterogeneous lesions that must be further characterised. MDPI 2023-01-29 /pmc/articles/PMC9916790/ /pubmed/36768889 http://dx.doi.org/10.3390/ijms24032567 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Piga, Isabella
L’Imperio, Vincenzo
Principi, Lucrezia
Bellevicine, Claudio
Fusco, Nicola
Maffini, Fausto
Venetis, Konstantinos
Ivanova, Mariia
Seminati, Davide
Casati, Gabriele
Pagani, Lisa
Galimberti, Stefania
Capitoli, Giulia
Garancini, Mattia
Gatti, Andrea-Valer
Magni, Fulvio
Pagni, Fabio
Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title_full Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title_fullStr Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title_full_unstemmed Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title_short Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)
title_sort spatially resolved molecular approaches for the characterisation of non-invasive follicular tumours with papillary-like features (niftps)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916790/
https://www.ncbi.nlm.nih.gov/pubmed/36768889
http://dx.doi.org/10.3390/ijms24032567
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