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Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia
The effects of aging on the nervous system are well documented. However, most previous studies on this topic were performed on the central nervous system. The present study was carried out on the dorsal root ganglia (DRGs) of mice, and focused on age-related changes in DRG neurons and satellite glia...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916822/ https://www.ncbi.nlm.nih.gov/pubmed/36769006 http://dx.doi.org/10.3390/ijms24032677 |
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author | Hanani, Menachem Spray, David C. Huang, Tian-Ying |
author_facet | Hanani, Menachem Spray, David C. Huang, Tian-Ying |
author_sort | Hanani, Menachem |
collection | PubMed |
description | The effects of aging on the nervous system are well documented. However, most previous studies on this topic were performed on the central nervous system. The present study was carried out on the dorsal root ganglia (DRGs) of mice, and focused on age-related changes in DRG neurons and satellite glial cells (SGCs). Intracellular electrodes were used for dye injection to examine the gap junction-mediated coupling between neurons and SGCs, and for intracellular electrical recordings from the neurons. Tactile sensitivity was assessed with von Frey hairs. We found that 3-23% of DRG neurons were dye-coupled to SGCs surrounding neighboring neurons in 8–24-month (Mo)-old mice, whereas in young adult (3 Mo) mice, the figure was 0%. The threshold current for firing an action potential in sensory neurons was significantly lower in DRGs from 12 Mo mice compared with those from 3 Mo mice. The percentage of neurons with spontaneous subthreshold membrane potential oscillation was greater by two-fold in 12 Mo mice. The withdrawal threshold was lower by 22% in 12 Mo mice compared with 3 Mo ones. These results show that in the aged mice, a proportion of DRG neurons is coupled to SGCs, and that the membrane excitability of the DRG neurons increases with age. We propose that augmented neuron–SGC communications via gap junctions are caused by low-grade inflammation associated with aging, and this may contribute to pain behavior. |
format | Online Article Text |
id | pubmed-9916822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99168222023-02-11 Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia Hanani, Menachem Spray, David C. Huang, Tian-Ying Int J Mol Sci Article The effects of aging on the nervous system are well documented. However, most previous studies on this topic were performed on the central nervous system. The present study was carried out on the dorsal root ganglia (DRGs) of mice, and focused on age-related changes in DRG neurons and satellite glial cells (SGCs). Intracellular electrodes were used for dye injection to examine the gap junction-mediated coupling between neurons and SGCs, and for intracellular electrical recordings from the neurons. Tactile sensitivity was assessed with von Frey hairs. We found that 3-23% of DRG neurons were dye-coupled to SGCs surrounding neighboring neurons in 8–24-month (Mo)-old mice, whereas in young adult (3 Mo) mice, the figure was 0%. The threshold current for firing an action potential in sensory neurons was significantly lower in DRGs from 12 Mo mice compared with those from 3 Mo mice. The percentage of neurons with spontaneous subthreshold membrane potential oscillation was greater by two-fold in 12 Mo mice. The withdrawal threshold was lower by 22% in 12 Mo mice compared with 3 Mo ones. These results show that in the aged mice, a proportion of DRG neurons is coupled to SGCs, and that the membrane excitability of the DRG neurons increases with age. We propose that augmented neuron–SGC communications via gap junctions are caused by low-grade inflammation associated with aging, and this may contribute to pain behavior. MDPI 2023-01-31 /pmc/articles/PMC9916822/ /pubmed/36769006 http://dx.doi.org/10.3390/ijms24032677 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hanani, Menachem Spray, David C. Huang, Tian-Ying Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title | Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title_full | Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title_fullStr | Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title_full_unstemmed | Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title_short | Age-Related Changes in Neurons and Satellite Glial Cells in Mouse Dorsal Root Ganglia |
title_sort | age-related changes in neurons and satellite glial cells in mouse dorsal root ganglia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916822/ https://www.ncbi.nlm.nih.gov/pubmed/36769006 http://dx.doi.org/10.3390/ijms24032677 |
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