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Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds

The cross-linking of structural proteins is critical for establishing the mechanical stability of the epithelial compartments of the skin and skin appendages. The introduction of isopeptide bonds between glutamine and lysine residues depends on catalysis by transglutaminases and represents the main...

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Autores principales: Sachslehner, Attila Placido, Surbek, Marta, Golabi, Bahar, Geiselhofer, Miriam, Jäger, Karin, Hess, Claudia, Kuchler, Ulrike, Gruber, Reinhard, Eckhart, Leopold
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916842/
https://www.ncbi.nlm.nih.gov/pubmed/36768511
http://dx.doi.org/10.3390/ijms24032193
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author Sachslehner, Attila Placido
Surbek, Marta
Golabi, Bahar
Geiselhofer, Miriam
Jäger, Karin
Hess, Claudia
Kuchler, Ulrike
Gruber, Reinhard
Eckhart, Leopold
author_facet Sachslehner, Attila Placido
Surbek, Marta
Golabi, Bahar
Geiselhofer, Miriam
Jäger, Karin
Hess, Claudia
Kuchler, Ulrike
Gruber, Reinhard
Eckhart, Leopold
author_sort Sachslehner, Attila Placido
collection PubMed
description The cross-linking of structural proteins is critical for establishing the mechanical stability of the epithelial compartments of the skin and skin appendages. The introduction of isopeptide bonds between glutamine and lysine residues depends on catalysis by transglutaminases and represents the main protein cross-linking mechanism besides the formation of disulfide bonds. Here, we used a fluorescent labeling protocol to localize the activity of transglutaminases on thin sections of the integument and its appendages in mammals and birds. In human tissues, transglutaminase activity was detected in the granular layer of the epidermis, suprabasal layers of the gingival epithelium, the duct of sweat glands, hair follicles and the nail matrix. In the skin appendages of chickens, transglutaminase activity was present in the claw matrix, the feather follicle sheath, the feather sheath and in differentiating keratinocytes of feather barb ridges. During chicken embryogenesis, active transglutaminase was found in the cornifying epidermis, the periderm and the subperiderm. Transglutaminase activity was also detected in the filiform papillae on the tongue of mice and in conical papillae on the tongue of chickens. In summary, our study reveals that transglutaminase activities are widely distributed in integumentary structures and suggests that transglutamination contributes to the cornification of hard skin appendages such as nails and feathers.
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spelling pubmed-99168422023-02-11 Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds Sachslehner, Attila Placido Surbek, Marta Golabi, Bahar Geiselhofer, Miriam Jäger, Karin Hess, Claudia Kuchler, Ulrike Gruber, Reinhard Eckhart, Leopold Int J Mol Sci Article The cross-linking of structural proteins is critical for establishing the mechanical stability of the epithelial compartments of the skin and skin appendages. The introduction of isopeptide bonds between glutamine and lysine residues depends on catalysis by transglutaminases and represents the main protein cross-linking mechanism besides the formation of disulfide bonds. Here, we used a fluorescent labeling protocol to localize the activity of transglutaminases on thin sections of the integument and its appendages in mammals and birds. In human tissues, transglutaminase activity was detected in the granular layer of the epidermis, suprabasal layers of the gingival epithelium, the duct of sweat glands, hair follicles and the nail matrix. In the skin appendages of chickens, transglutaminase activity was present in the claw matrix, the feather follicle sheath, the feather sheath and in differentiating keratinocytes of feather barb ridges. During chicken embryogenesis, active transglutaminase was found in the cornifying epidermis, the periderm and the subperiderm. Transglutaminase activity was also detected in the filiform papillae on the tongue of mice and in conical papillae on the tongue of chickens. In summary, our study reveals that transglutaminase activities are widely distributed in integumentary structures and suggests that transglutamination contributes to the cornification of hard skin appendages such as nails and feathers. MDPI 2023-01-22 /pmc/articles/PMC9916842/ /pubmed/36768511 http://dx.doi.org/10.3390/ijms24032193 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sachslehner, Attila Placido
Surbek, Marta
Golabi, Bahar
Geiselhofer, Miriam
Jäger, Karin
Hess, Claudia
Kuchler, Ulrike
Gruber, Reinhard
Eckhart, Leopold
Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title_full Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title_fullStr Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title_full_unstemmed Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title_short Transglutaminase Activity Is Conserved in Stratified Epithelia and Skin Appendages of Mammals and Birds
title_sort transglutaminase activity is conserved in stratified epithelia and skin appendages of mammals and birds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916842/
https://www.ncbi.nlm.nih.gov/pubmed/36768511
http://dx.doi.org/10.3390/ijms24032193
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