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Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics fo...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916851/ https://www.ncbi.nlm.nih.gov/pubmed/36768739 http://dx.doi.org/10.3390/ijms24032418 |
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author | Pudova, Elena A. Kobelyatskaya, Anastasiya A. Katunina, Irina V. Snezhkina, Anastasiya V. Fedorova, Maria S. Pavlov, Vladislav S. Bakhtogarimov, Ildar R. Lantsova, Margarita S. Kokin, Sergey P. Nyushko, Kirill M. Alekseev, Boris Ya. Kalinin, Dmitry V. Melnikova, Nataliya V. Dmitriev, Alexey A. Krasnov, George S. Kudryavtseva, Anna V. |
author_facet | Pudova, Elena A. Kobelyatskaya, Anastasiya A. Katunina, Irina V. Snezhkina, Anastasiya V. Fedorova, Maria S. Pavlov, Vladislav S. Bakhtogarimov, Ildar R. Lantsova, Margarita S. Kokin, Sergey P. Nyushko, Kirill M. Alekseev, Boris Ya. Kalinin, Dmitry V. Melnikova, Nataliya V. Dmitriev, Alexey A. Krasnov, George S. Kudryavtseva, Anna V. |
author_sort | Pudova, Elena A. |
collection | PubMed |
description | Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics for treating a patient are insufficiently informative and need to be supplemented with new markers. In this study, we performed RNA-Seq of PCa tissue samples, aimed at identifying potential prognostic markers at the level of gene expression and miRNAs associated with one of the key signs of cancer aggressiveness—lymphatic dissemination. The relative expression of candidate markers was validated by quantitative PCR, including an independent sample of patients based on archival material. Statistically significant results, derived from an independent set of samples, were confirmed for miR-148a-3p and miR-615-3p, as well as for the CST2, OCLN, and PCAT4 genes. Considering the obtained validation data, we also analyzed the predictive value of models based on various combinations of identified markers using algorithms based on machine learning. The highest predictive potential was shown for the “CST2 + OCLN + pT” model (AUC = 0.863) based on the CatBoost Classifier algorithm. |
format | Online Article Text |
id | pubmed-9916851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99168512023-02-11 Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models Pudova, Elena A. Kobelyatskaya, Anastasiya A. Katunina, Irina V. Snezhkina, Anastasiya V. Fedorova, Maria S. Pavlov, Vladislav S. Bakhtogarimov, Ildar R. Lantsova, Margarita S. Kokin, Sergey P. Nyushko, Kirill M. Alekseev, Boris Ya. Kalinin, Dmitry V. Melnikova, Nataliya V. Dmitriev, Alexey A. Krasnov, George S. Kudryavtseva, Anna V. Int J Mol Sci Article Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics for treating a patient are insufficiently informative and need to be supplemented with new markers. In this study, we performed RNA-Seq of PCa tissue samples, aimed at identifying potential prognostic markers at the level of gene expression and miRNAs associated with one of the key signs of cancer aggressiveness—lymphatic dissemination. The relative expression of candidate markers was validated by quantitative PCR, including an independent sample of patients based on archival material. Statistically significant results, derived from an independent set of samples, were confirmed for miR-148a-3p and miR-615-3p, as well as for the CST2, OCLN, and PCAT4 genes. Considering the obtained validation data, we also analyzed the predictive value of models based on various combinations of identified markers using algorithms based on machine learning. The highest predictive potential was shown for the “CST2 + OCLN + pT” model (AUC = 0.863) based on the CatBoost Classifier algorithm. MDPI 2023-01-26 /pmc/articles/PMC9916851/ /pubmed/36768739 http://dx.doi.org/10.3390/ijms24032418 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pudova, Elena A. Kobelyatskaya, Anastasiya A. Katunina, Irina V. Snezhkina, Anastasiya V. Fedorova, Maria S. Pavlov, Vladislav S. Bakhtogarimov, Ildar R. Lantsova, Margarita S. Kokin, Sergey P. Nyushko, Kirill M. Alekseev, Boris Ya. Kalinin, Dmitry V. Melnikova, Nataliya V. Dmitriev, Alexey A. Krasnov, George S. Kudryavtseva, Anna V. Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title | Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title_full | Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title_fullStr | Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title_full_unstemmed | Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title_short | Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models |
title_sort | lymphatic dissemination in prostate cancer: features of the transcriptomic profile and prognostic models |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916851/ https://www.ncbi.nlm.nih.gov/pubmed/36768739 http://dx.doi.org/10.3390/ijms24032418 |
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