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Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models

Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics fo...

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Autores principales: Pudova, Elena A., Kobelyatskaya, Anastasiya A., Katunina, Irina V., Snezhkina, Anastasiya V., Fedorova, Maria S., Pavlov, Vladislav S., Bakhtogarimov, Ildar R., Lantsova, Margarita S., Kokin, Sergey P., Nyushko, Kirill M., Alekseev, Boris Ya., Kalinin, Dmitry V., Melnikova, Nataliya V., Dmitriev, Alexey A., Krasnov, George S., Kudryavtseva, Anna V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916851/
https://www.ncbi.nlm.nih.gov/pubmed/36768739
http://dx.doi.org/10.3390/ijms24032418
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author Pudova, Elena A.
Kobelyatskaya, Anastasiya A.
Katunina, Irina V.
Snezhkina, Anastasiya V.
Fedorova, Maria S.
Pavlov, Vladislav S.
Bakhtogarimov, Ildar R.
Lantsova, Margarita S.
Kokin, Sergey P.
Nyushko, Kirill M.
Alekseev, Boris Ya.
Kalinin, Dmitry V.
Melnikova, Nataliya V.
Dmitriev, Alexey A.
Krasnov, George S.
Kudryavtseva, Anna V.
author_facet Pudova, Elena A.
Kobelyatskaya, Anastasiya A.
Katunina, Irina V.
Snezhkina, Anastasiya V.
Fedorova, Maria S.
Pavlov, Vladislav S.
Bakhtogarimov, Ildar R.
Lantsova, Margarita S.
Kokin, Sergey P.
Nyushko, Kirill M.
Alekseev, Boris Ya.
Kalinin, Dmitry V.
Melnikova, Nataliya V.
Dmitriev, Alexey A.
Krasnov, George S.
Kudryavtseva, Anna V.
author_sort Pudova, Elena A.
collection PubMed
description Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics for treating a patient are insufficiently informative and need to be supplemented with new markers. In this study, we performed RNA-Seq of PCa tissue samples, aimed at identifying potential prognostic markers at the level of gene expression and miRNAs associated with one of the key signs of cancer aggressiveness—lymphatic dissemination. The relative expression of candidate markers was validated by quantitative PCR, including an independent sample of patients based on archival material. Statistically significant results, derived from an independent set of samples, were confirmed for miR-148a-3p and miR-615-3p, as well as for the CST2, OCLN, and PCAT4 genes. Considering the obtained validation data, we also analyzed the predictive value of models based on various combinations of identified markers using algorithms based on machine learning. The highest predictive potential was shown for the “CST2 + OCLN + pT” model (AUC = 0.863) based on the CatBoost Classifier algorithm.
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spelling pubmed-99168512023-02-11 Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models Pudova, Elena A. Kobelyatskaya, Anastasiya A. Katunina, Irina V. Snezhkina, Anastasiya V. Fedorova, Maria S. Pavlov, Vladislav S. Bakhtogarimov, Ildar R. Lantsova, Margarita S. Kokin, Sergey P. Nyushko, Kirill M. Alekseev, Boris Ya. Kalinin, Dmitry V. Melnikova, Nataliya V. Dmitriev, Alexey A. Krasnov, George S. Kudryavtseva, Anna V. Int J Mol Sci Article Radical prostatectomy is the gold standard treatment for prostate cancer (PCa); however, it does not always completely cure PCa, and patients often experience a recurrence of the disease. In addition, the clinical and pathological parameters used to assess the prognosis and choose further tactics for treating a patient are insufficiently informative and need to be supplemented with new markers. In this study, we performed RNA-Seq of PCa tissue samples, aimed at identifying potential prognostic markers at the level of gene expression and miRNAs associated with one of the key signs of cancer aggressiveness—lymphatic dissemination. The relative expression of candidate markers was validated by quantitative PCR, including an independent sample of patients based on archival material. Statistically significant results, derived from an independent set of samples, were confirmed for miR-148a-3p and miR-615-3p, as well as for the CST2, OCLN, and PCAT4 genes. Considering the obtained validation data, we also analyzed the predictive value of models based on various combinations of identified markers using algorithms based on machine learning. The highest predictive potential was shown for the “CST2 + OCLN + pT” model (AUC = 0.863) based on the CatBoost Classifier algorithm. MDPI 2023-01-26 /pmc/articles/PMC9916851/ /pubmed/36768739 http://dx.doi.org/10.3390/ijms24032418 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pudova, Elena A.
Kobelyatskaya, Anastasiya A.
Katunina, Irina V.
Snezhkina, Anastasiya V.
Fedorova, Maria S.
Pavlov, Vladislav S.
Bakhtogarimov, Ildar R.
Lantsova, Margarita S.
Kokin, Sergey P.
Nyushko, Kirill M.
Alekseev, Boris Ya.
Kalinin, Dmitry V.
Melnikova, Nataliya V.
Dmitriev, Alexey A.
Krasnov, George S.
Kudryavtseva, Anna V.
Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title_full Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title_fullStr Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title_full_unstemmed Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title_short Lymphatic Dissemination in Prostate Cancer: Features of the Transcriptomic Profile and Prognostic Models
title_sort lymphatic dissemination in prostate cancer: features of the transcriptomic profile and prognostic models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916851/
https://www.ncbi.nlm.nih.gov/pubmed/36768739
http://dx.doi.org/10.3390/ijms24032418
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