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Evaluation of CRISPR/Cas9 Constructs in Wheat Cell Suspension Cultures

Despite intensive optimization efforts, developing an efficient sequence-specific CRISPR/Cas-mediated genome editing method remains a challenge, especially in polyploid cereal species such as wheat. Validating the efficacy of nuclease constructs prior to using them in planta is, thus, a major step o...

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Detalles Bibliográficos
Autores principales: Michalski, Krzysztof, Ziąbska, Paulina, Sowa, Sławomir, Zimny, Janusz, Linkiewicz, Anna M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916915/
https://www.ncbi.nlm.nih.gov/pubmed/36768486
http://dx.doi.org/10.3390/ijms24032162