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A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing

The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide mutations cannot be discriminated from wild-type alleles by current CRISPR-Cas systems. Here, we functionally screened six Cas12j nucleas...

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Detalles Bibliográficos
Autores principales:	Wang, Yao, Qi, Tao, Liu, Jingtong, Yang, Yuan, Wang, Ziwen, Wang, Ying, Wang, Tianyi, Li, Miaomiao, Li, Mingqing, Lu, Daru, Chang, Alex Chia Yu, Yang, Li, Gao, Song, Wang, Yongming, Lan, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2023
Materias:	Biomedicine and Life Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917002/ https://www.ncbi.nlm.nih.gov/pubmed/36763662 http://dx.doi.org/10.1126/sciadv.abo6405

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