Cargando…

A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing

The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide mutations cannot be discriminated from wild-type alleles by current CRISPR-Cas systems. Here, we functionally screened six Cas12j nucleas...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Yao, Qi, Tao, Liu, Jingtong, Yang, Yuan, Wang, Ziwen, Wang, Ying, Wang, Tianyi, Li, Miaomiao, Li, Mingqing, Lu, Daru, Chang, Alex Chia Yu, Yang, Li, Gao, Song, Wang, Yongming, Lan, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2023
Materias:	Biomedicine and Life Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917002/ https://www.ncbi.nlm.nih.gov/pubmed/36763662 http://dx.doi.org/10.1126/sciadv.abo6405

Ejemplares similares

Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system
por: Shiriaeva, Anna A., et al.
Publicado: (2022)

A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
por: Wang, Xinyi, et al.
Publicado: (2021)

Efficient cancer modeling through CRISPR-Cas9/HDR-based somatic precision gene editing in mice
por: Bu, Wen, et al.
Publicado: (2023)

Hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy
por: Fan, Ningke, et al.
Publicado: (2022)

Genome-editing prodrug: Targeted delivery and conditional stabilization of CRISPR-Cas9 for precision therapy of inflammatory disease
por: Yan, Xiaojie, et al.
Publicado: (2021)

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing
por: Park, So Hyun, et al.
Publicado: (2022)

Human SAMD9 is a poxvirus-activatable anticodon nuclease inhibiting codon-specific protein synthesis
por: Zhang, Fushun, et al.
Publicado: (2023)

In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapy
por: Han, Jeong Pil, et al.
Publicado: (2022)

Application of CRISPR/Cas9 Nuclease in Amphioxus Genome Editing
por: Su, Liuru, et al.
Publicado: (2020)

Buffering of genetic dominance by allele-specific protein complex assembly
por: Badonyi, Mihaly, et al.
Publicado: (2023)

Targeted gene silencing in the nervous system with CRISPR-Cas13
por: Powell, Jackson E., et al.
Publicado: (2022)

Recombination affects allele-specific expression of deleterious variants in human populations
por: Harwood, Michelle P., et al.
Publicado: (2022)

Blood-brain barrier–penetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy
por: Zou, Yan, et al.
Publicado: (2022)

Analysis of bacterial pangenomes reduces CRISPR dark matter and reveals strong association between membranome and CRISPR-Cas systems
por: Rubio, Alejandro, et al.
Publicado: (2023)

Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing
por: Chen, Yangcan, et al.
Publicado: (2022)

CRISPR clear? Dimeric Cas9-Fok1 nucleases improve genome-editing specificity
por: Zhang, Hongmei, et al.
Publicado: (2014)

Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
por: Huang, Xiaoqiang, et al.
Publicado: (2022)

An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
por: Hansen, Stine L., et al.
Publicado: (2023)

Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells
por: Tran, Ngoc Tung, et al.
Publicado: (2022)

A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila
por: Doll, Roman M., et al.
Publicado: (2023)

Cell activity modulation and its specific function maintenance by bioinspired electromechanical nanogenerator
por: Li, Tong, et al.
Publicado: (2021)

Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
por: Wan, Tao, et al.
Publicado: (2022)

Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis
por: Kang, Yu, et al.
Publicado: (2022)

Liver cancer heterogeneity modeled by in situ genome editing of hepatocytes
por: Tang, Mei, et al.
Publicado: (2022)

Engineered CRISPR-Cas9 nucleases with altered PAM specificities
por: Kleinstiver, Benjamin P., et al.
Publicado: (2015)

Primary specification of blastocyst trophectoderm by scRNA-seq: New insights into embryo implantation
por: Liu, Dandan, et al.
Publicado: (2022)

Application of combined CRISPR screening for genetic and chemical-genetic interaction profiling in Mycobacterium tuberculosis
por: Yan, Mei-Yi, et al.
Publicado: (2022)

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
por: Li, Yanhe, et al.
Publicado: (2020)

A nitrogen fixing symbiosis-specific pathway required for legume flowering
por: Yun, Jinxia, et al.
Publicado: (2023)

Gut-targeted nanoparticles deliver specifically targeted antimicrobial peptides against Clostridium perfringens infections
por: Xu, Bocheng, et al.
Publicado: (2023)

Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting
por: Lu, Xukun, et al.
Publicado: (2021)

CRISPR-Cas9 screen reveals a role of purine synthesis for estrogen receptor α activity and tamoxifen resistance of breast cancer cells
por: Hany, Dina, et al.
Publicado: (2023)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

FOXG1 sequentially orchestrates subtype specification of postmitotic cortical projection neurons
por: Liu, Junhua, et al.
Publicado: (2022)

NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification
por: Yang, Yongkang, et al.
Publicado: (2022)

Receptor-specific recognition of NPY peptides revealed by structures of NPY receptors
por: Tang, Tingting, et al.
Publicado: (2022)

High-temperature adaptation of an OsNRT2.3 allele is thermoregulated by small RNAs
por: Zhang, Yong, et al.
Publicado: (2022)

Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections
por: Paredes-Redondo, Amaia, et al.
Publicado: (2021)

ZMP recruits and excludes Pol IV–mediated DNA methylation in a site-specific manner
por: Wang, Yuan, et al.
Publicado: (2022)

Genome-wide CRISPR screens using isogenic cells reveal vulnerabilities conferred by loss of tumor suppressors
por: Feng, Xu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ickn4qbe52mfiu8eb7m8gjs23a