Cargando…

SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents

Parahydrogen (p-H(2))-based techniques are known to drastically enhance NMR signals but are usually limited by p-H(2) supply. This work reports p-H(2)-based SABRE hyperpolarization at p-H(2) pressures of hundreds of bar, far beyond the typical ten bar currently reported in the literature. A recently...

Descripción completa

Detalles Bibliográficos
Autores principales: Duchowny, Anton, Denninger, Johannes, Lohmann, Lars, Theis, Thomas, Lehmkuhl, Sören, Adams, Alina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917027/
https://www.ncbi.nlm.nih.gov/pubmed/36768786
http://dx.doi.org/10.3390/ijms24032465
_version_ 1784886270296588288
author Duchowny, Anton
Denninger, Johannes
Lohmann, Lars
Theis, Thomas
Lehmkuhl, Sören
Adams, Alina
author_facet Duchowny, Anton
Denninger, Johannes
Lohmann, Lars
Theis, Thomas
Lehmkuhl, Sören
Adams, Alina
author_sort Duchowny, Anton
collection PubMed
description Parahydrogen (p-H(2))-based techniques are known to drastically enhance NMR signals but are usually limited by p-H(2) supply. This work reports p-H(2)-based SABRE hyperpolarization at p-H(2) pressures of hundreds of bar, far beyond the typical ten bar currently reported in the literature. A recently designed high-pressure setup was utilized to compress p-H(2) gas up to 200 bar. The measurements were conducted using a sapphire high-pressure NMR tube and a 43 MHz benchtop NMR spectrometer. In standard methanol solutions, it could be shown that the signal intensities increased with pressure until they eventually reached a plateau. A polarization of about 2%, equal to a molar polarization of 1.2 mmol L(−1), could be achieved for the sample with the highest substrate concentration. While the signal plateaued, the H(2) solubility increased linearly with pressure from 1 to 200 bar, indicating that p-H(2) availability is not the limiting factor in signal enhancement beyond a certain pressure, depending on sample composition. Furthermore, the possibility of using liquefied ethane and compressed CO(2) as removable solvents for hyperpolarization was demonstrated. The use of high pressures together with quickly removable organic/non-organic solvents represents an important breakthrough in the field of hyperpolarization, advancing SABRE as a promising tool for materials science, biophysics, and molecular imaging.
format Online
Article
Text
id pubmed-9917027
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99170272023-02-11 SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents Duchowny, Anton Denninger, Johannes Lohmann, Lars Theis, Thomas Lehmkuhl, Sören Adams, Alina Int J Mol Sci Communication Parahydrogen (p-H(2))-based techniques are known to drastically enhance NMR signals but are usually limited by p-H(2) supply. This work reports p-H(2)-based SABRE hyperpolarization at p-H(2) pressures of hundreds of bar, far beyond the typical ten bar currently reported in the literature. A recently designed high-pressure setup was utilized to compress p-H(2) gas up to 200 bar. The measurements were conducted using a sapphire high-pressure NMR tube and a 43 MHz benchtop NMR spectrometer. In standard methanol solutions, it could be shown that the signal intensities increased with pressure until they eventually reached a plateau. A polarization of about 2%, equal to a molar polarization of 1.2 mmol L(−1), could be achieved for the sample with the highest substrate concentration. While the signal plateaued, the H(2) solubility increased linearly with pressure from 1 to 200 bar, indicating that p-H(2) availability is not the limiting factor in signal enhancement beyond a certain pressure, depending on sample composition. Furthermore, the possibility of using liquefied ethane and compressed CO(2) as removable solvents for hyperpolarization was demonstrated. The use of high pressures together with quickly removable organic/non-organic solvents represents an important breakthrough in the field of hyperpolarization, advancing SABRE as a promising tool for materials science, biophysics, and molecular imaging. MDPI 2023-01-27 /pmc/articles/PMC9917027/ /pubmed/36768786 http://dx.doi.org/10.3390/ijms24032465 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Duchowny, Anton
Denninger, Johannes
Lohmann, Lars
Theis, Thomas
Lehmkuhl, Sören
Adams, Alina
SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title_full SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title_fullStr SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title_full_unstemmed SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title_short SABRE Hyperpolarization with up to 200 bar Parahydrogen in Standard and Quickly Removable Solvents
title_sort sabre hyperpolarization with up to 200 bar parahydrogen in standard and quickly removable solvents
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917027/
https://www.ncbi.nlm.nih.gov/pubmed/36768786
http://dx.doi.org/10.3390/ijms24032465
work_keys_str_mv AT duchownyanton sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents
AT denningerjohannes sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents
AT lohmannlars sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents
AT theisthomas sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents
AT lehmkuhlsoren sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents
AT adamsalina sabrehyperpolarizationwithupto200barparahydrogeninstandardandquicklyremovablesolvents