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Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research

Musculoskeletal disorders, including fractures, scoliosis, heterotopic ossification, osteoporosis, osteoarthritis, disc degeneration, and muscular injury, etc., can occur at any stage of human life. Understanding the occurrence and development mechanism of musculoskeletal disorders, as well as the c...

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Detalles Bibliográficos
Autores principales: Feng, Site, Li, Jiahao, Tian, Jingjing, Lu, Sheng, Zhao, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917071/
https://www.ncbi.nlm.nih.gov/pubmed/36768592
http://dx.doi.org/10.3390/ijms24032271
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author Feng, Site
Li, Jiahao
Tian, Jingjing
Lu, Sheng
Zhao, Yu
author_facet Feng, Site
Li, Jiahao
Tian, Jingjing
Lu, Sheng
Zhao, Yu
author_sort Feng, Site
collection PubMed
description Musculoskeletal disorders, including fractures, scoliosis, heterotopic ossification, osteoporosis, osteoarthritis, disc degeneration, and muscular injury, etc., can occur at any stage of human life. Understanding the occurrence and development mechanism of musculoskeletal disorders, as well as the changes in tissues and cells during therapy, might help us find targeted treatment methods. Single-cell techniques provide excellent tools for studying alterations at the cellular level of disorders. However, the application of these techniques in research on musculoskeletal disorders is still limited. This review summarizes the current single-cell and spatial omics used in musculoskeletal disorders. Cell isolation, experimental methods, and feasible experimental designs for single-cell studies of musculoskeletal system diseases have been reviewed based on tissue characteristics. Then, the paper summarizes the latest findings of single-cell studies in musculoskeletal disorders from three aspects: bone and ossification, joint, and muscle and tendon disorders. Recent discoveries about the cell populations involved in these diseases are highlighted. Furthermore, the therapeutic responses of musculoskeletal disorders, especially single-cell changes after the treatments of implants, stem cell therapies, and drugs are described. Finally, the application potential and future development directions of single-cell and spatial omics in research on musculoskeletal diseases are discussed.
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spelling pubmed-99170712023-02-11 Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research Feng, Site Li, Jiahao Tian, Jingjing Lu, Sheng Zhao, Yu Int J Mol Sci Review Musculoskeletal disorders, including fractures, scoliosis, heterotopic ossification, osteoporosis, osteoarthritis, disc degeneration, and muscular injury, etc., can occur at any stage of human life. Understanding the occurrence and development mechanism of musculoskeletal disorders, as well as the changes in tissues and cells during therapy, might help us find targeted treatment methods. Single-cell techniques provide excellent tools for studying alterations at the cellular level of disorders. However, the application of these techniques in research on musculoskeletal disorders is still limited. This review summarizes the current single-cell and spatial omics used in musculoskeletal disorders. Cell isolation, experimental methods, and feasible experimental designs for single-cell studies of musculoskeletal system diseases have been reviewed based on tissue characteristics. Then, the paper summarizes the latest findings of single-cell studies in musculoskeletal disorders from three aspects: bone and ossification, joint, and muscle and tendon disorders. Recent discoveries about the cell populations involved in these diseases are highlighted. Furthermore, the therapeutic responses of musculoskeletal disorders, especially single-cell changes after the treatments of implants, stem cell therapies, and drugs are described. Finally, the application potential and future development directions of single-cell and spatial omics in research on musculoskeletal diseases are discussed. MDPI 2023-01-23 /pmc/articles/PMC9917071/ /pubmed/36768592 http://dx.doi.org/10.3390/ijms24032271 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Feng, Site
Li, Jiahao
Tian, Jingjing
Lu, Sheng
Zhao, Yu
Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title_full Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title_fullStr Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title_full_unstemmed Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title_short Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research
title_sort application of single-cell and spatial omics in musculoskeletal disorder research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917071/
https://www.ncbi.nlm.nih.gov/pubmed/36768592
http://dx.doi.org/10.3390/ijms24032271
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