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MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development
AP3 has been studied and is reported to affect structural changes in floral organs in various plants. However, the function of the soybean AP3 genes in flower development is unknown. Here, the full-length cDNA sequence of GmAP3 was obtained by RACE and it was verified that it belongs to the MADS-box...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917172/ https://www.ncbi.nlm.nih.gov/pubmed/36769078 http://dx.doi.org/10.3390/ijms24032751 |
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author | Zhang, Aijing He, Haobo Li, Yue Wang, Lixue Liu, Yixuan Luan, Xinchao Wang, Jiaxin Liu, Huijing Liu, Shuying Zhang, Jun Yao, Dan |
author_facet | Zhang, Aijing He, Haobo Li, Yue Wang, Lixue Liu, Yixuan Luan, Xinchao Wang, Jiaxin Liu, Huijing Liu, Shuying Zhang, Jun Yao, Dan |
author_sort | Zhang, Aijing |
collection | PubMed |
description | AP3 has been studied and is reported to affect structural changes in floral organs in various plants. However, the function of the soybean AP3 genes in flower development is unknown. Here, the full-length cDNA sequence of GmAP3 was obtained by RACE and it was verified that it belongs to the MADS-box subfamily by a bioinformatics analysis. The expression of GmAP3 is closely related to the expression of essential enzyme genes related to flower development. Yeast two-hybrid assays demonstrated that GmAP3 interacts with AP1 to determine the identity of flower organ development. A follow-up analysis showed that overexpression of the GmAP3 gene advanced flowering time and resulted in changes in floral organ morphology. The average flowering time of overexpressed soybean and tobacco plants was 6–8 days earlier than that of wild-type plants, and the average flowering time of gene-edited soybean and tobacco plants was 6–11 days later than that of wild-type plants. In conclusion, GmAP3 may directly or indirectly affect the flower development of soybean. The results of this study lay the foundation for further research on the biological functions of MADS transcriptional factors in soybeans. |
format | Online Article Text |
id | pubmed-9917172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99171722023-02-11 MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development Zhang, Aijing He, Haobo Li, Yue Wang, Lixue Liu, Yixuan Luan, Xinchao Wang, Jiaxin Liu, Huijing Liu, Shuying Zhang, Jun Yao, Dan Int J Mol Sci Article AP3 has been studied and is reported to affect structural changes in floral organs in various plants. However, the function of the soybean AP3 genes in flower development is unknown. Here, the full-length cDNA sequence of GmAP3 was obtained by RACE and it was verified that it belongs to the MADS-box subfamily by a bioinformatics analysis. The expression of GmAP3 is closely related to the expression of essential enzyme genes related to flower development. Yeast two-hybrid assays demonstrated that GmAP3 interacts with AP1 to determine the identity of flower organ development. A follow-up analysis showed that overexpression of the GmAP3 gene advanced flowering time and resulted in changes in floral organ morphology. The average flowering time of overexpressed soybean and tobacco plants was 6–8 days earlier than that of wild-type plants, and the average flowering time of gene-edited soybean and tobacco plants was 6–11 days later than that of wild-type plants. In conclusion, GmAP3 may directly or indirectly affect the flower development of soybean. The results of this study lay the foundation for further research on the biological functions of MADS transcriptional factors in soybeans. MDPI 2023-02-01 /pmc/articles/PMC9917172/ /pubmed/36769078 http://dx.doi.org/10.3390/ijms24032751 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Aijing He, Haobo Li, Yue Wang, Lixue Liu, Yixuan Luan, Xinchao Wang, Jiaxin Liu, Huijing Liu, Shuying Zhang, Jun Yao, Dan MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title | MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title_full | MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title_fullStr | MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title_full_unstemmed | MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title_short | MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development |
title_sort | mads-box subfamily gene gmap3 from glycine max regulates early flowering and flower development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917172/ https://www.ncbi.nlm.nih.gov/pubmed/36769078 http://dx.doi.org/10.3390/ijms24032751 |
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