N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

N6-methyladenosine (m(6)A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m(6)A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs p...

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Detalles Bibliográficos
Autores principales: Murata, Takayuki, Iwahori, Satoko, Okuno, Yusuke, Nishitsuji, Hironori, Yanagi, Yusuke, Watashi, Koichi, Wakita, Takaji, Kimura, Hiroshi, Shimotohno, Kunitada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917364/
https://www.ncbi.nlm.nih.gov/pubmed/36768585
http://dx.doi.org/10.3390/ijms24032265
Descripción
Sumario:N6-methyladenosine (m(6)A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m(6)A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m(6)A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m(6)A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.

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