N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx

N6-methyladenosine (m(6)A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m(6)A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs p...

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Autores principales: Murata, Takayuki, Iwahori, Satoko, Okuno, Yusuke, Nishitsuji, Hironori, Yanagi, Yusuke, Watashi, Koichi, Wakita, Takaji, Kimura, Hiroshi, Shimotohno, Kunitada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917364/
https://www.ncbi.nlm.nih.gov/pubmed/36768585
http://dx.doi.org/10.3390/ijms24032265
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author Murata, Takayuki
Iwahori, Satoko
Okuno, Yusuke
Nishitsuji, Hironori
Yanagi, Yusuke
Watashi, Koichi
Wakita, Takaji
Kimura, Hiroshi
Shimotohno, Kunitada
author_facet Murata, Takayuki
Iwahori, Satoko
Okuno, Yusuke
Nishitsuji, Hironori
Yanagi, Yusuke
Watashi, Koichi
Wakita, Takaji
Kimura, Hiroshi
Shimotohno, Kunitada
author_sort Murata, Takayuki
collection PubMed
description N6-methyladenosine (m(6)A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m(6)A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m(6)A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m(6)A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.
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spelling pubmed-99173642023-02-11 N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx Murata, Takayuki Iwahori, Satoko Okuno, Yusuke Nishitsuji, Hironori Yanagi, Yusuke Watashi, Koichi Wakita, Takaji Kimura, Hiroshi Shimotohno, Kunitada Int J Mol Sci Article N6-methyladenosine (m(6)A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m(6)A predominantly in the coding region of HBx. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m(6)A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m(6)A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes. MDPI 2023-01-23 /pmc/articles/PMC9917364/ /pubmed/36768585 http://dx.doi.org/10.3390/ijms24032265 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murata, Takayuki
Iwahori, Satoko
Okuno, Yusuke
Nishitsuji, Hironori
Yanagi, Yusuke
Watashi, Koichi
Wakita, Takaji
Kimura, Hiroshi
Shimotohno, Kunitada
N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title_full N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title_fullStr N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title_full_unstemmed N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title_short N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of HBx
title_sort n6-methyladenosine modification of hepatitis b virus rna in the coding region of hbx
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917364/
https://www.ncbi.nlm.nih.gov/pubmed/36768585
http://dx.doi.org/10.3390/ijms24032265
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