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A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus

The tuberal hypothalamus controls life-supporting homeostatic processes, but despite its fundamental role, the cells and signalling pathways that specify this unique region of the central nervous system in embryogenesis are poorly characterised. Here, we combine experimental and bioinformatic approa...

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Autores principales: Chinnaiya, Kavitha, Burbridge, Sarah, Jones, Aragorn, Kim, Dong Won, Place, Elsie, Manning, Elizabeth, Groves, Ian, Sun, Changyu, Towers, Matthew, Blackshaw, Seth, Placzek, Marysia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917434/
https://www.ncbi.nlm.nih.gov/pubmed/36718990
http://dx.doi.org/10.7554/eLife.83133
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author Chinnaiya, Kavitha
Burbridge, Sarah
Jones, Aragorn
Kim, Dong Won
Place, Elsie
Manning, Elizabeth
Groves, Ian
Sun, Changyu
Towers, Matthew
Blackshaw, Seth
Placzek, Marysia
author_facet Chinnaiya, Kavitha
Burbridge, Sarah
Jones, Aragorn
Kim, Dong Won
Place, Elsie
Manning, Elizabeth
Groves, Ian
Sun, Changyu
Towers, Matthew
Blackshaw, Seth
Placzek, Marysia
author_sort Chinnaiya, Kavitha
collection PubMed
description The tuberal hypothalamus controls life-supporting homeostatic processes, but despite its fundamental role, the cells and signalling pathways that specify this unique region of the central nervous system in embryogenesis are poorly characterised. Here, we combine experimental and bioinformatic approaches in the embryonic chick to show that the tuberal hypothalamus is progressively generated from hypothalamic floor plate-like cells. Fate-mapping studies show that a stream of tuberal progenitors develops in the anterior-ventral neural tube as a wave of neuroepithelial-derived BMP signalling sweeps from anterior to posterior through the hypothalamic floor plate. As later-specified posterior tuberal progenitors are generated, early specified anterior tuberal progenitors become progressively more distant from these BMP signals and differentiate into tuberal neurogenic cells. Gain- and loss-of-function experiments in vivo and ex vivo show that BMP signalling initiates tuberal progenitor specification, but must be eliminated for these to progress to anterior neurogenic progenitors. scRNA-Seq profiling shows that tuberal progenitors that are specified after the major period of anterior tuberal specification begin to upregulate genes that characterise radial glial cells. This study provides an integrated account of the development of the tuberal hypothalamus.
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spelling pubmed-99174342023-02-11 A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus Chinnaiya, Kavitha Burbridge, Sarah Jones, Aragorn Kim, Dong Won Place, Elsie Manning, Elizabeth Groves, Ian Sun, Changyu Towers, Matthew Blackshaw, Seth Placzek, Marysia eLife Developmental Biology The tuberal hypothalamus controls life-supporting homeostatic processes, but despite its fundamental role, the cells and signalling pathways that specify this unique region of the central nervous system in embryogenesis are poorly characterised. Here, we combine experimental and bioinformatic approaches in the embryonic chick to show that the tuberal hypothalamus is progressively generated from hypothalamic floor plate-like cells. Fate-mapping studies show that a stream of tuberal progenitors develops in the anterior-ventral neural tube as a wave of neuroepithelial-derived BMP signalling sweeps from anterior to posterior through the hypothalamic floor plate. As later-specified posterior tuberal progenitors are generated, early specified anterior tuberal progenitors become progressively more distant from these BMP signals and differentiate into tuberal neurogenic cells. Gain- and loss-of-function experiments in vivo and ex vivo show that BMP signalling initiates tuberal progenitor specification, but must be eliminated for these to progress to anterior neurogenic progenitors. scRNA-Seq profiling shows that tuberal progenitors that are specified after the major period of anterior tuberal specification begin to upregulate genes that characterise radial glial cells. This study provides an integrated account of the development of the tuberal hypothalamus. eLife Sciences Publications, Ltd 2023-02-10 /pmc/articles/PMC9917434/ /pubmed/36718990 http://dx.doi.org/10.7554/eLife.83133 Text en © 2023, Chinnaiya et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Chinnaiya, Kavitha
Burbridge, Sarah
Jones, Aragorn
Kim, Dong Won
Place, Elsie
Manning, Elizabeth
Groves, Ian
Sun, Changyu
Towers, Matthew
Blackshaw, Seth
Placzek, Marysia
A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title_full A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title_fullStr A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title_full_unstemmed A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title_short A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus
title_sort neuroepithelial wave of bmp signalling drives anteroposterior specification of the tuberal hypothalamus
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917434/
https://www.ncbi.nlm.nih.gov/pubmed/36718990
http://dx.doi.org/10.7554/eLife.83133
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