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Circulating Microbial Cell-Free DNA in Health and Disease

Human blood contains low biomass of circulating microbial cell-free DNA (cfmDNA) that predominantly originates from bacteria. Numerous studies have detected circulating cfmDNA in patients with infectious and non-infectious diseases, and in healthy individuals. Remarkable differences were found in th...

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Autores principales: Pietrzak, Bernadeta, Kawacka, Iwona, Olejnik-Schmidt, Agnieszka, Schmidt, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917616/
https://www.ncbi.nlm.nih.gov/pubmed/36769374
http://dx.doi.org/10.3390/ijms24033051
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author Pietrzak, Bernadeta
Kawacka, Iwona
Olejnik-Schmidt, Agnieszka
Schmidt, Marcin
author_facet Pietrzak, Bernadeta
Kawacka, Iwona
Olejnik-Schmidt, Agnieszka
Schmidt, Marcin
author_sort Pietrzak, Bernadeta
collection PubMed
description Human blood contains low biomass of circulating microbial cell-free DNA (cfmDNA) that predominantly originates from bacteria. Numerous studies have detected circulating cfmDNA in patients with infectious and non-infectious diseases, and in healthy individuals. Remarkable differences were found in the microbial composition of healthy subjects and patients compared to cohorts with various diseases or even patients with diversified prognoses, implying that these alterations may be associated with disease development. Although the function of circulating cfmDNA needs to be elucidated (whether it acts as a bystander of dysbiosis or a key player in disease development), several studies have demonstrated its potential as a non-invasive biomarker that may improve diagnosis and treatment efficacy. The origin of circulating cfmDNA is still the subject of much deliberation, but studies have identified members of various microbiome niches, including the gut, oral cavity, airways, and skin. Further studies investigating the origin and function of circulating cfmDNA are needed. Moreover, low-biomass microbiome studies are prone to contamination, therefore stringent negative experimental control reactions and decontamination frameworks are advised in order to detect genuine circulating cfmDNA.
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spelling pubmed-99176162023-02-11 Circulating Microbial Cell-Free DNA in Health and Disease Pietrzak, Bernadeta Kawacka, Iwona Olejnik-Schmidt, Agnieszka Schmidt, Marcin Int J Mol Sci Review Human blood contains low biomass of circulating microbial cell-free DNA (cfmDNA) that predominantly originates from bacteria. Numerous studies have detected circulating cfmDNA in patients with infectious and non-infectious diseases, and in healthy individuals. Remarkable differences were found in the microbial composition of healthy subjects and patients compared to cohorts with various diseases or even patients with diversified prognoses, implying that these alterations may be associated with disease development. Although the function of circulating cfmDNA needs to be elucidated (whether it acts as a bystander of dysbiosis or a key player in disease development), several studies have demonstrated its potential as a non-invasive biomarker that may improve diagnosis and treatment efficacy. The origin of circulating cfmDNA is still the subject of much deliberation, but studies have identified members of various microbiome niches, including the gut, oral cavity, airways, and skin. Further studies investigating the origin and function of circulating cfmDNA are needed. Moreover, low-biomass microbiome studies are prone to contamination, therefore stringent negative experimental control reactions and decontamination frameworks are advised in order to detect genuine circulating cfmDNA. MDPI 2023-02-03 /pmc/articles/PMC9917616/ /pubmed/36769374 http://dx.doi.org/10.3390/ijms24033051 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pietrzak, Bernadeta
Kawacka, Iwona
Olejnik-Schmidt, Agnieszka
Schmidt, Marcin
Circulating Microbial Cell-Free DNA in Health and Disease
title Circulating Microbial Cell-Free DNA in Health and Disease
title_full Circulating Microbial Cell-Free DNA in Health and Disease
title_fullStr Circulating Microbial Cell-Free DNA in Health and Disease
title_full_unstemmed Circulating Microbial Cell-Free DNA in Health and Disease
title_short Circulating Microbial Cell-Free DNA in Health and Disease
title_sort circulating microbial cell-free dna in health and disease
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917616/
https://www.ncbi.nlm.nih.gov/pubmed/36769374
http://dx.doi.org/10.3390/ijms24033051
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