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Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System

Aquaporin-4 (AQP4) is the most abundant water channel in the central nervous system and plays a fundamental role in maintaining water homeostasis there. In adult mice, AQP4 is located mainly in ependymal cells, in the endfeet of perivascular astrocytes, and in the glia limitans. Meanwhile, its expre...

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Autores principales: Mayo, Francisco, González-Vinceiro, Lourdes, Hiraldo-González, Laura, Calle-Castillejo, Claudia, Morales-Alvarez, Sara, Ramírez-Lorca, Reposo, Echevarría, Miriam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917791/
https://www.ncbi.nlm.nih.gov/pubmed/36769371
http://dx.doi.org/10.3390/ijms24033048
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author Mayo, Francisco
González-Vinceiro, Lourdes
Hiraldo-González, Laura
Calle-Castillejo, Claudia
Morales-Alvarez, Sara
Ramírez-Lorca, Reposo
Echevarría, Miriam
author_facet Mayo, Francisco
González-Vinceiro, Lourdes
Hiraldo-González, Laura
Calle-Castillejo, Claudia
Morales-Alvarez, Sara
Ramírez-Lorca, Reposo
Echevarría, Miriam
author_sort Mayo, Francisco
collection PubMed
description Aquaporin-4 (AQP4) is the most abundant water channel in the central nervous system and plays a fundamental role in maintaining water homeostasis there. In adult mice, AQP4 is located mainly in ependymal cells, in the endfeet of perivascular astrocytes, and in the glia limitans. Meanwhile, its expression, location, and function throughout postnatal development remain largely unknown. Here, the expression of AQP4 mRNA was studied by in situ hybridization and RT-qPCR, and the localization and amount of protein was studied by immunofluorescence and western blotting, both in the brain and spinal cord. For this, wild-type mice of the C57BL/6 line, aged 1, 3, 7, 11, 20, and 60 days, and 18 months were used. The results showed a change in both the expression and location of AQP4 in postnatal development compared to those during adult life. In the early stages of postnatal development it appears in highly myelinated areas, such as the corpus callosum or cerebellum, and as the animal grows, it disappears from these areas, passing through the cortical regions of the forebrain and concentrating around the blood vessels. These findings suggest an unprecedented possible role for AQP4 in the early cell differentiation process, during the first days of life in the newborn animal, which will lead to myelination.
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spelling pubmed-99177912023-02-11 Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System Mayo, Francisco González-Vinceiro, Lourdes Hiraldo-González, Laura Calle-Castillejo, Claudia Morales-Alvarez, Sara Ramírez-Lorca, Reposo Echevarría, Miriam Int J Mol Sci Article Aquaporin-4 (AQP4) is the most abundant water channel in the central nervous system and plays a fundamental role in maintaining water homeostasis there. In adult mice, AQP4 is located mainly in ependymal cells, in the endfeet of perivascular astrocytes, and in the glia limitans. Meanwhile, its expression, location, and function throughout postnatal development remain largely unknown. Here, the expression of AQP4 mRNA was studied by in situ hybridization and RT-qPCR, and the localization and amount of protein was studied by immunofluorescence and western blotting, both in the brain and spinal cord. For this, wild-type mice of the C57BL/6 line, aged 1, 3, 7, 11, 20, and 60 days, and 18 months were used. The results showed a change in both the expression and location of AQP4 in postnatal development compared to those during adult life. In the early stages of postnatal development it appears in highly myelinated areas, such as the corpus callosum or cerebellum, and as the animal grows, it disappears from these areas, passing through the cortical regions of the forebrain and concentrating around the blood vessels. These findings suggest an unprecedented possible role for AQP4 in the early cell differentiation process, during the first days of life in the newborn animal, which will lead to myelination. MDPI 2023-02-03 /pmc/articles/PMC9917791/ /pubmed/36769371 http://dx.doi.org/10.3390/ijms24033048 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mayo, Francisco
González-Vinceiro, Lourdes
Hiraldo-González, Laura
Calle-Castillejo, Claudia
Morales-Alvarez, Sara
Ramírez-Lorca, Reposo
Echevarría, Miriam
Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title_full Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title_fullStr Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title_full_unstemmed Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title_short Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System
title_sort aquaporin-4 expression switches from white to gray matter regions during postnatal development of the central nervous system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917791/
https://www.ncbi.nlm.nih.gov/pubmed/36769371
http://dx.doi.org/10.3390/ijms24033048
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