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Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study

The aim of this in vitro study was to investigate the effect of hydrogen peroxide (H(2)O(2)) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and rough...

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Autores principales: Tuna, Taskin, Wein, Martin, Altmann, Brigitte, Steinberg, Thorsten, Fischer, Jens, Att, Wael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918077/
https://www.ncbi.nlm.nih.gov/pubmed/36769968
http://dx.doi.org/10.3390/ma16030961
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author Tuna, Taskin
Wein, Martin
Altmann, Brigitte
Steinberg, Thorsten
Fischer, Jens
Att, Wael
author_facet Tuna, Taskin
Wein, Martin
Altmann, Brigitte
Steinberg, Thorsten
Fischer, Jens
Att, Wael
author_sort Tuna, Taskin
collection PubMed
description The aim of this in vitro study was to investigate the effect of hydrogen peroxide (H(2)O(2)) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and roughened surfaces were immersed in 20% H(2)O(2) for two hours. Scanning electron and atomic force microscopy showed no topographic changes after H(2)O(2)-treatment. Contact angle measurements (1), X-ray photoelectron spectroscopy (2) and X-ray diffraction (3) indicated that H(2)O(2)-treated surfaces (1) increased in hydrophilicity (p < 0.05) and (2) on three surfaces the carbon content decreased (33–60%), while (3) the monoclinic phase increased on all surfaces. Immunofluorescence analysis of the cell area and DNA-quantification and alkaline phosphatase activity revealed no effect of H(2)O(2)-treatment on cell behavior. Proliferation activity was significantly higher on three of the four untreated surfaces, especially on the smooth surfaces (p < 0.05). Within the limitations of this study, it can be concluded that exposure of zirconia surfaces to 20% H(2)O(2) for 2 h increases the wettability of the surfaces, but also seems to increase the monoclinic phase, especially on roughened surfaces, which can be considered detrimental to material stability. Moreover, the H(2)O(2)-treatment has no influence on osteoblast behavior.
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spelling pubmed-99180772023-02-11 Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study Tuna, Taskin Wein, Martin Altmann, Brigitte Steinberg, Thorsten Fischer, Jens Att, Wael Materials (Basel) Article The aim of this in vitro study was to investigate the effect of hydrogen peroxide (H(2)O(2)) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and roughened surfaces were immersed in 20% H(2)O(2) for two hours. Scanning electron and atomic force microscopy showed no topographic changes after H(2)O(2)-treatment. Contact angle measurements (1), X-ray photoelectron spectroscopy (2) and X-ray diffraction (3) indicated that H(2)O(2)-treated surfaces (1) increased in hydrophilicity (p < 0.05) and (2) on three surfaces the carbon content decreased (33–60%), while (3) the monoclinic phase increased on all surfaces. Immunofluorescence analysis of the cell area and DNA-quantification and alkaline phosphatase activity revealed no effect of H(2)O(2)-treatment on cell behavior. Proliferation activity was significantly higher on three of the four untreated surfaces, especially on the smooth surfaces (p < 0.05). Within the limitations of this study, it can be concluded that exposure of zirconia surfaces to 20% H(2)O(2) for 2 h increases the wettability of the surfaces, but also seems to increase the monoclinic phase, especially on roughened surfaces, which can be considered detrimental to material stability. Moreover, the H(2)O(2)-treatment has no influence on osteoblast behavior. MDPI 2023-01-20 /pmc/articles/PMC9918077/ /pubmed/36769968 http://dx.doi.org/10.3390/ma16030961 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tuna, Taskin
Wein, Martin
Altmann, Brigitte
Steinberg, Thorsten
Fischer, Jens
Att, Wael
Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title_full Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title_fullStr Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title_full_unstemmed Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title_short Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: An In Vitro Study
title_sort effect of hydrogen peroxide on the surface and attractiveness of various zirconia implant materials on human osteoblasts: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918077/
https://www.ncbi.nlm.nih.gov/pubmed/36769968
http://dx.doi.org/10.3390/ma16030961
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