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Endogenous DNase Activity in an Animal Model of Acute Liver Failure

Deoxyribonucleases (DNases) cleave extracellular DNA (ecDNA) and are under intense research as interventions for diseases associated with high ecDNA, such as acute live injury. DNase I treatment decreases morbidity and mortality in this animal model. Endogenous DNase activity has high interindividua...

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Autores principales: Janovičová, Ľubica, Kmeťová, Katarína, Pribulová, Nikola, Janko, Jakub, Gromová, Barbora, Gardlík, Roman, Celec, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918174/
https://www.ncbi.nlm.nih.gov/pubmed/36769306
http://dx.doi.org/10.3390/ijms24032984
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author Janovičová, Ľubica
Kmeťová, Katarína
Pribulová, Nikola
Janko, Jakub
Gromová, Barbora
Gardlík, Roman
Celec, Peter
author_facet Janovičová, Ľubica
Kmeťová, Katarína
Pribulová, Nikola
Janko, Jakub
Gromová, Barbora
Gardlík, Roman
Celec, Peter
author_sort Janovičová, Ľubica
collection PubMed
description Deoxyribonucleases (DNases) cleave extracellular DNA (ecDNA) and are under intense research as interventions for diseases associated with high ecDNA, such as acute live injury. DNase I treatment decreases morbidity and mortality in this animal model. Endogenous DNase activity has high interindividual variability. In this study, we tested the hypothesis that high endogenous DNase activity is beneficial in an animal model of acute liver failure. DNase activity was measured in the plasma of adult male mice taken before i.p. injection of thioacetamide to induce acute liver failure. The survival of mice was monitored for 48 h. Mice were retrospectively divided into two groups based on the median DNase activity assessed using the gel-based single-radial enzyme diffusion assay. In acute liver failure, mice with a higher baseline DNase activity had lower mortality after 48 h (by 25%). Different protection of ecDNA against nucleases by vesicles or DNA-binding proteins could play a role and should be further evaluated. Similarly, the role of endogenous DNase activity should be analyzed in other disease models associated with high ecDNA.
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spelling pubmed-99181742023-02-11 Endogenous DNase Activity in an Animal Model of Acute Liver Failure Janovičová, Ľubica Kmeťová, Katarína Pribulová, Nikola Janko, Jakub Gromová, Barbora Gardlík, Roman Celec, Peter Int J Mol Sci Communication Deoxyribonucleases (DNases) cleave extracellular DNA (ecDNA) and are under intense research as interventions for diseases associated with high ecDNA, such as acute live injury. DNase I treatment decreases morbidity and mortality in this animal model. Endogenous DNase activity has high interindividual variability. In this study, we tested the hypothesis that high endogenous DNase activity is beneficial in an animal model of acute liver failure. DNase activity was measured in the plasma of adult male mice taken before i.p. injection of thioacetamide to induce acute liver failure. The survival of mice was monitored for 48 h. Mice were retrospectively divided into two groups based on the median DNase activity assessed using the gel-based single-radial enzyme diffusion assay. In acute liver failure, mice with a higher baseline DNase activity had lower mortality after 48 h (by 25%). Different protection of ecDNA against nucleases by vesicles or DNA-binding proteins could play a role and should be further evaluated. Similarly, the role of endogenous DNase activity should be analyzed in other disease models associated with high ecDNA. MDPI 2023-02-03 /pmc/articles/PMC9918174/ /pubmed/36769306 http://dx.doi.org/10.3390/ijms24032984 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Janovičová, Ľubica
Kmeťová, Katarína
Pribulová, Nikola
Janko, Jakub
Gromová, Barbora
Gardlík, Roman
Celec, Peter
Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title_full Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title_fullStr Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title_full_unstemmed Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title_short Endogenous DNase Activity in an Animal Model of Acute Liver Failure
title_sort endogenous dnase activity in an animal model of acute liver failure
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918174/
https://www.ncbi.nlm.nih.gov/pubmed/36769306
http://dx.doi.org/10.3390/ijms24032984
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