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Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study

Periodontitis is a chronic disease with a high overall prevalence. It involves a complex interplay between the immune-inflammatory pathways and biofilm changes, leading to periodontal attachment loss. The aims of this study were (i) to assess whether the salivary IL-1β, IL-17A, RANK-L and OPG levels...

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Autores principales: Relvas, Marta, Silvestre, Ricardo, Gonçalves, Maria, Cabral, Cristina, Mendes-Frias, Ana, Monteiro, Luís, Viana da Costa, Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918260/
https://www.ncbi.nlm.nih.gov/pubmed/36769650
http://dx.doi.org/10.3390/jcm12031003
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author Relvas, Marta
Silvestre, Ricardo
Gonçalves, Maria
Cabral, Cristina
Mendes-Frias, Ana
Monteiro, Luís
Viana da Costa, Alexandra
author_facet Relvas, Marta
Silvestre, Ricardo
Gonçalves, Maria
Cabral, Cristina
Mendes-Frias, Ana
Monteiro, Luís
Viana da Costa, Alexandra
author_sort Relvas, Marta
collection PubMed
description Periodontitis is a chronic disease with a high overall prevalence. It involves a complex interplay between the immune-inflammatory pathways and biofilm changes, leading to periodontal attachment loss. The aims of this study were (i) to assess whether the salivary IL-1β, IL-17A, RANK-L and OPG levels have the potential to discriminate between the mild and severe periodontitis conditions; and (ii) to enable diagnostic/prognostic actions to differentiate between distinct levels of the disease. The analysis of the clinical parameters and the evaluation of the salivary immunomediators levels by means of a multiplex flow assay revealed a statistically significantly higher level of IL-1β in the periodontitis III/IV patients, as well as a higher level of RANK-L in the periodontitis III/IV and I/II patients, when compared to the healthy controls. Furthermore, the grade C periodontitis patients presented a significantly higher level of RANK-L compared to the grade B and grade A patients. In the grade C patients, IL-1β had a positive correlation with the PPD and CAL indices and RANK_L had a positive correlation with CAL. The evidence emerging from this study associates the salivary IL-1β and RANK-L levels with an advanced stage of periodontitis, stage III/IV, and with grade C, suggesting the possible cooperative action of both in the inflammatory and bone loss events. In addition to IL-1β, RANK-L could be considered a combined diagnostic biomarker for periodontitis.
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spelling pubmed-99182602023-02-11 Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study Relvas, Marta Silvestre, Ricardo Gonçalves, Maria Cabral, Cristina Mendes-Frias, Ana Monteiro, Luís Viana da Costa, Alexandra J Clin Med Article Periodontitis is a chronic disease with a high overall prevalence. It involves a complex interplay between the immune-inflammatory pathways and biofilm changes, leading to periodontal attachment loss. The aims of this study were (i) to assess whether the salivary IL-1β, IL-17A, RANK-L and OPG levels have the potential to discriminate between the mild and severe periodontitis conditions; and (ii) to enable diagnostic/prognostic actions to differentiate between distinct levels of the disease. The analysis of the clinical parameters and the evaluation of the salivary immunomediators levels by means of a multiplex flow assay revealed a statistically significantly higher level of IL-1β in the periodontitis III/IV patients, as well as a higher level of RANK-L in the periodontitis III/IV and I/II patients, when compared to the healthy controls. Furthermore, the grade C periodontitis patients presented a significantly higher level of RANK-L compared to the grade B and grade A patients. In the grade C patients, IL-1β had a positive correlation with the PPD and CAL indices and RANK_L had a positive correlation with CAL. The evidence emerging from this study associates the salivary IL-1β and RANK-L levels with an advanced stage of periodontitis, stage III/IV, and with grade C, suggesting the possible cooperative action of both in the inflammatory and bone loss events. In addition to IL-1β, RANK-L could be considered a combined diagnostic biomarker for periodontitis. MDPI 2023-01-28 /pmc/articles/PMC9918260/ /pubmed/36769650 http://dx.doi.org/10.3390/jcm12031003 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Relvas, Marta
Silvestre, Ricardo
Gonçalves, Maria
Cabral, Cristina
Mendes-Frias, Ana
Monteiro, Luís
Viana da Costa, Alexandra
Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title_full Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title_fullStr Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title_full_unstemmed Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title_short Analysis of Salivary Levels of IL-1β, IL17A, OPG and RANK-L in Periodontitis Using the 2017 Classification of Periodontal Diseases—An Exploratory Observational Study
title_sort analysis of salivary levels of il-1β, il17a, opg and rank-l in periodontitis using the 2017 classification of periodontal diseases—an exploratory observational study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918260/
https://www.ncbi.nlm.nih.gov/pubmed/36769650
http://dx.doi.org/10.3390/jcm12031003
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