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Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study

Apatite cement (AC), which has excellent osteoconductive ability, and alpha-tricalcium phosphate (α-TCP), which can be used for bone replacement, are useful bone substitute materials. The objective of this study was to clarify the physical properties and antimicrobial release ability of antibiotic-l...

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Autores principales: Sasaki, Kazuki, Ninomiya, Yoshiaki, Takechi, Masaaki, Tsuru, Kanji, Ishikawa, Kunio, Shigeishi, Hideo, Ohta, Kouji, Aikawa, Tomonao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918266/
https://www.ncbi.nlm.nih.gov/pubmed/36770002
http://dx.doi.org/10.3390/ma16030995
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author Sasaki, Kazuki
Ninomiya, Yoshiaki
Takechi, Masaaki
Tsuru, Kanji
Ishikawa, Kunio
Shigeishi, Hideo
Ohta, Kouji
Aikawa, Tomonao
author_facet Sasaki, Kazuki
Ninomiya, Yoshiaki
Takechi, Masaaki
Tsuru, Kanji
Ishikawa, Kunio
Shigeishi, Hideo
Ohta, Kouji
Aikawa, Tomonao
author_sort Sasaki, Kazuki
collection PubMed
description Apatite cement (AC), which has excellent osteoconductive ability, and alpha-tricalcium phosphate (α-TCP), which can be used for bone replacement, are useful bone substitute materials. The objective of this study was to clarify the physical properties and antimicrobial release ability of antibiotic-loaded AC/α-TCP composites in vitro. Gentamicin-loaded, rapid setting AC/α-TCP composites were prepared in 2 mixing ratios (10:3 and 10:6). The cement paste of AC/α-TCP composites was prepared in a plastic mold and dried in a thermostatic chamber at 37 °C and 100% relative humidity for 24 h. A diametral tensile strength test, powder X-ray diffraction analysis, and gentamicin release test were performed. The diametral tensile strengths of the AC/α-TCP composites were significantly less than that of AC alone. Powder X-ray diffraction patterns exhibited the characteristic peaks of hydroxyapatite in the AC/α-TCP composites and gentamicin-loaded AC/α-TCP composites. The concentration of the released gentamicin was maintained above the minimum inhibitory concentration of Staphylococcus aureus until Day 30 in both the gentamicin-loaded AC/α-TCP composites (10:3 and 10:6). Our results suggest that a gentamicin-loaded AC/α-TCP composite has potential as a drug delivery system. Further study is essential to investigate the antimicrobial activity and safety of the gentamicin-loaded AC/α-TCP composites in animal models.
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spelling pubmed-99182662023-02-11 Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study Sasaki, Kazuki Ninomiya, Yoshiaki Takechi, Masaaki Tsuru, Kanji Ishikawa, Kunio Shigeishi, Hideo Ohta, Kouji Aikawa, Tomonao Materials (Basel) Article Apatite cement (AC), which has excellent osteoconductive ability, and alpha-tricalcium phosphate (α-TCP), which can be used for bone replacement, are useful bone substitute materials. The objective of this study was to clarify the physical properties and antimicrobial release ability of antibiotic-loaded AC/α-TCP composites in vitro. Gentamicin-loaded, rapid setting AC/α-TCP composites were prepared in 2 mixing ratios (10:3 and 10:6). The cement paste of AC/α-TCP composites was prepared in a plastic mold and dried in a thermostatic chamber at 37 °C and 100% relative humidity for 24 h. A diametral tensile strength test, powder X-ray diffraction analysis, and gentamicin release test were performed. The diametral tensile strengths of the AC/α-TCP composites were significantly less than that of AC alone. Powder X-ray diffraction patterns exhibited the characteristic peaks of hydroxyapatite in the AC/α-TCP composites and gentamicin-loaded AC/α-TCP composites. The concentration of the released gentamicin was maintained above the minimum inhibitory concentration of Staphylococcus aureus until Day 30 in both the gentamicin-loaded AC/α-TCP composites (10:3 and 10:6). Our results suggest that a gentamicin-loaded AC/α-TCP composite has potential as a drug delivery system. Further study is essential to investigate the antimicrobial activity and safety of the gentamicin-loaded AC/α-TCP composites in animal models. MDPI 2023-01-21 /pmc/articles/PMC9918266/ /pubmed/36770002 http://dx.doi.org/10.3390/ma16030995 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sasaki, Kazuki
Ninomiya, Yoshiaki
Takechi, Masaaki
Tsuru, Kanji
Ishikawa, Kunio
Shigeishi, Hideo
Ohta, Kouji
Aikawa, Tomonao
Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title_full Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title_fullStr Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title_full_unstemmed Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title_short Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study
title_sort physical properties and antimicrobial release ability of gentamicin-loaded apatite cement/α-tcp composites: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9918266/
https://www.ncbi.nlm.nih.gov/pubmed/36770002
http://dx.doi.org/10.3390/ma16030995
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