Cargando…

Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel

Site-directed spin labeling (SDSL) combined with continuous wave electron paramagnetic resonance (cw EPR) spectroscopy is a powerful technique to reveal, at the local level, the dynamics of structural transitions in proteins. Here, we consider SDSL-EPR based on the selective grafting of a nitroxide...

Descripción completa

Detalles Bibliográficos
Autores principales:	Etienne, Emilien, Pierro, Annalisa, Tamburrini, Ketty C., Bonucci, Alessio, Mileo, Elisabetta, Martinho, Marlène, Belle, Valérie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919594/ https://www.ncbi.nlm.nih.gov/pubmed/36771013 http://dx.doi.org/10.3390/molecules28031348

Ejemplares similares

In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR
por: Pierro, Annalisa, et al.
Publicado: (2023)

A Comparison of Cysteine-Conjugated Nitroxide Spin Labels for Pulse Dipolar EPR Spectroscopy
por: Ackermann, Katrin, et al.
Publicado: (2021)

The spatial effect of protein deuteration on nitroxide spin-label relaxation: Implications for EPR distance measurement
por: El Mkami, Hassane, et al.
Publicado: (2014)

gem‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility
por: Bleicken, Stephanie, et al.
Publicado: (2019)

Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy
por: Le Breton, Nolwenn, et al.
Publicado: (2015)

Spin-labelled mechanically interlocked molecules as models for the interpretation of biradical EPR spectra
por: Gualandi, Lorenzo, et al.
Publicado: (2021)

Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins; the Cu(II)- Cu(II) and Nitroxide-Nitroxide Cases
por: Ackermann, Katrin, et al.
Publicado: (2021)

Conformation of bis-nitroxide polarizing agents by multi-frequency EPR spectroscopy()
por: Soetbeer, Janne, et al.
Publicado: (2018)

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra
por: Bartucci, Rosa, et al.
Publicado: (2022)

Correction: Conformation of bis-nitroxide polarizing agents by multi-frequency EPR spectroscopy
por: Soetbeer, Janne, et al.
Publicado: (2020)

New Developments in Spin Labels for Pulsed Dipolar EPR
por: Fielding, Alistair J., et al.
Publicado: (2014)

Phosphoramidite building blocks with protected nitroxides for the synthesis of spin-labeled DNA and RNA
por: Weinrich, Timo, et al.
Publicado: (2018)

Protein Spin Labeling with a Photocaged Nitroxide Using Diels–Alder Chemistry
por: Kugele, Anandi, et al.
Publicado: (2019)

Geometry and water accessibility of the inhibitor binding site of Na(+)-pump: Pulse- and CW-EPR study
por: Aloi, Erika, et al.
Publicado: (2021)

Isoindoline‐Based Nitroxides as Bioresistant Spin Labels for Protein Labeling through Cysteines and Alkyne‐Bearing Noncanonical Amino Acids
por: Braun, Theresa Sophie, et al.
Publicado: (2019)

RosettaEPR: Rotamer Library for Spin Label Structure and Dynamics
por: Alexander, Nathan S., et al.
Publicado: (2013)

The use of the Rx spin label in orientation measurement on proteins, by EPR
por: Stevens, M. A., et al.
Publicado: (2016)

Site-Directed Spin Labeling EPR for Studying Membrane Proteins
por: Sahu, Indra D., et al.
Publicado: (2018)

Effects of Curcumin on Lipid Membranes: an EPR Spin-label Study
por: Duda, Mariusz, et al.
Publicado: (2020)

Probing Columnar Discotic Liquid Crystals by EPR Spectroscopy with a Rigid-Core Nitroxide Spin Probe**
por: Gopee, Hemant, et al.
Publicado: (2013)

Solvent Effects on Skin Penetration and Spatial Distribution of the Hydrophilic Nitroxide Spin Probe PCA Investigated by EPR
por: Dong, Pin, et al.
Publicado: (2020)

A facile method for attaching nitroxide spin labels at the 5′ terminus of nucleic acids(†)
por: Grant, Gian Paola G., et al.
Publicado: (2007)

A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids
por: Nguyen, Phuong H, et al.
Publicado: (2015)

Human Serum Albumin Labelled with Sterically-Hindered Nitroxides as Potential MRI Contrast Agents
por: Dobrynin, Sergey, et al.
Publicado: (2020)

Correction to: Solvent Effects on Skin Penetration and Spatial Distribution of the Hydrophilic Nitroxide Spin Probe PCA Investigated by EPR
por: Dong, Pin, et al.
Publicado: (2021)

Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility
por: Pierro, Annalisa, et al.
Publicado: (2020)

In Vivo Evaluation of Indium-111–Labeled 800CW as a Necrosis-Avid Contrast Agent
por: Stroet, Marcus C. M., et al.
Publicado: (2020)

A Simulation Independent Analysis of Single- and Multi-Component cw ESR Spectra
por: Roy, Aritro Sinha, et al.
Publicado: (2023)

Spin-Label EPR for Determining Polarity and Proticity in Biomolecular Assemblies: Transmembrane Profiles
por: Marsh, Derek
Publicado: (2009)

The relationship between folding and activity in UreG, an intrinsically disordered enzyme
por: Palombo, Marta, et al.
Publicado: (2017)

DEER Sensitivity between Iron Centers and Nitroxides in Heme-Containing Proteins Improves Dramatically Using Broadband, High-Field EPR
por: Motion, Claire L., et al.
Publicado: (2016)

Stationary-Phase EPR for Exploring Protein Structure, Conformation, and Dynamics in Spin-Labeled Proteins
por: López, Carlos J., et al.
Publicado: (2014)

Pulse EPR Measurements of Intramolecular Distances in a TOPP-Labeled Transmembrane Peptide in Lipids
por: Halbmair, Karin, et al.
Publicado: (2016)

DEER and RIDME Measurements of the Nitroxide-Spin Labelled Copper-Bound Amine Oxidase Homodimer from Arthrobacter Globiformis
por: Russell, Hannah, et al.
Publicado: (2021)

EPR Spectroscopy of a Clinically Active (1:2) Copper(II)-Histidine Complex Used in the Treatment of Menkes Disease: A Fourier Transform Analysis of a Fluid CW-EPR Spectrum
por: Gala, Lukas, et al.
Publicado: (2014)

Electroplated waveguides to enhance DNP and EPR spectra of silicon and diamond particles
por: Himmler, Aaron, et al.
Publicado: (2022)

Acidic and Electrosurface Properties of Binary TiO(2)-SiO(2) Xerogels Using EPR of pH-Sensitive Nitroxides
por: Antonov, Denis O., et al.
Publicado: (2021)

Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe
por: Cai, Qi, et al.
Publicado: (2006)

Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy
por: Widder, Pia, et al.
Publicado: (2019)

Is Cys(MTSL) the Best α-Amino Acid Residue to Electron Spin Labeling of Synthetically Accessible Peptide Molecules with Nitroxides?
por: Biondi, Barbara, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_rvqkapspmjiig7e6vf5cidft8i