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Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments
Due to its several economic and ecological consequences, biofouling is a widely recognized concern in the marine sector. The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919625/ https://www.ncbi.nlm.nih.gov/pubmed/36770342 http://dx.doi.org/10.3390/nano13030381 |
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author | Sousa-Cardoso, Francisca Teixeira-Santos, Rita Campos, Ana Francisca Lima, Marta Gomes, Luciana C. Soares, Olívia S. G. P. Mergulhão, Filipe J. |
author_facet | Sousa-Cardoso, Francisca Teixeira-Santos, Rita Campos, Ana Francisca Lima, Marta Gomes, Luciana C. Soares, Olívia S. G. P. Mergulhão, Filipe J. |
author_sort | Sousa-Cardoso, Francisca |
collection | PubMed |
description | Due to its several economic and ecological consequences, biofouling is a widely recognized concern in the marine sector. The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pristine graphene nanoplatelets (GNP) on biofilm development through the representative marine bacteria Cobetia marina and to investigate the antibacterial mechanisms of action of this material. For this purpose, a flow cytometric analysis was performed and a GNP/polydimethylsiloxane (PDMS) surface containing 5 wt% GNP (G5/PDMS) was produced, characterized, and assessed regarding its biofilm mitigation potential over 42 days in controlled hydrodynamic conditions that mimic marine environments. Flow cytometry revealed membrane damage, greater metabolic activity, and endogenous reactive oxygen species (ROS) production by C. marina when exposed to GNP 5% (w/v) for 24 h. In addition, C. marina biofilms formed on G5/PDMS showed consistently lower cell count and thickness (up to 43% reductions) than PDMS. Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS. Overall, the GNP-based surface inhibited C. marina biofilm development, showing promising potential as a marine antifouling coating. |
format | Online Article Text |
id | pubmed-9919625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99196252023-02-12 Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments Sousa-Cardoso, Francisca Teixeira-Santos, Rita Campos, Ana Francisca Lima, Marta Gomes, Luciana C. Soares, Olívia S. G. P. Mergulhão, Filipe J. Nanomaterials (Basel) Article Due to its several economic and ecological consequences, biofouling is a widely recognized concern in the marine sector. The search for non-biocide-release antifouling coatings has been on the rise, with carbon-nanocoated surfaces showing promising activity. This work aimed to study the impact of pristine graphene nanoplatelets (GNP) on biofilm development through the representative marine bacteria Cobetia marina and to investigate the antibacterial mechanisms of action of this material. For this purpose, a flow cytometric analysis was performed and a GNP/polydimethylsiloxane (PDMS) surface containing 5 wt% GNP (G5/PDMS) was produced, characterized, and assessed regarding its biofilm mitigation potential over 42 days in controlled hydrodynamic conditions that mimic marine environments. Flow cytometry revealed membrane damage, greater metabolic activity, and endogenous reactive oxygen species (ROS) production by C. marina when exposed to GNP 5% (w/v) for 24 h. In addition, C. marina biofilms formed on G5/PDMS showed consistently lower cell count and thickness (up to 43% reductions) than PDMS. Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS. Overall, the GNP-based surface inhibited C. marina biofilm development, showing promising potential as a marine antifouling coating. MDPI 2023-01-18 /pmc/articles/PMC9919625/ /pubmed/36770342 http://dx.doi.org/10.3390/nano13030381 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sousa-Cardoso, Francisca Teixeira-Santos, Rita Campos, Ana Francisca Lima, Marta Gomes, Luciana C. Soares, Olívia S. G. P. Mergulhão, Filipe J. Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title | Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title_full | Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title_fullStr | Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title_full_unstemmed | Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title_short | Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments |
title_sort | graphene-based coating to mitigate biofilm development in marine environments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919625/ https://www.ncbi.nlm.nih.gov/pubmed/36770342 http://dx.doi.org/10.3390/nano13030381 |
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