Cargando…

Characterization of Galectin Fusion Proteins with Glycoprotein Affinity Columns and Binding Assays

Galectins are β-galactosyl-binding proteins that fulfill essential physiological functions. In the biotechnological field, galectins are versatile tools, such as in the development of biomaterial coatings or the early-stage diagnosis of cancer diseases. Recently, we introduced galectin-1 (Gal-1) and...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dey, Carina, Palm, Philip, Elling, Lothar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919667/ https://www.ncbi.nlm.nih.gov/pubmed/36770718 http://dx.doi.org/10.3390/molecules28031054

Ejemplares similares

Galectin Binding to Neo-Glycoproteins: LacDiNAc Conjugated BSA as Ligand for Human Galectin-3
por: Böcker, Sophia, et al.
Publicado: (2015)

Enzymatic Synthesis of N-Acetyllactosamine (LacNAc) Type 1 Oligomers and Characterization as Multivalent Galectin Ligands
por: Fischöder, Thomas, et al.
Publicado: (2017)

Synthesis of the Thomsen-Friedenreich-antigen (TF-antigen) and binding of Galectin-3 to TF-antigen presenting neo-glycoproteins
por: Hoffmann, Marius, et al.
Publicado: (2020)

Poly-N-Acetyllactosamine Neo-Glycoproteins as Nanomolar Ligands of Human Galectin-3: Binding Kinetics and Modeling
por: Bumba, Ladislav, et al.
Publicado: (2018)

The Anti-angiogenic Peptide Anginex Greatly Enhances Galectin-1 Binding Affinity for Glycoproteins
por: Salomonsson, Emma, et al.
Publicado: (2011)

Galectin-8 in IgA Nephritis: Decreased Binding of IgA by Galectin-8 Affinity Chromatography and Associated Increased Binding in Non-IgA Serum Glycoproteins
por: Carlsson, Michael C., et al.
Publicado: (2011)

Sequence-Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin-3 and Delay Wound Healing of a Galectin-3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay
por: Freichel, Tanja, et al.
Publicado: (2020)

SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoproteins
por: Matthews, Alicia M., et al.
Publicado: (2022)

Biotinylated N-Acetyllactosamine- and N,N-Diacetyllactosamine-Based Oligosaccharides as Novel Ligands for Human Galectin-3 †
por: Böcker, Sophia, et al.
Publicado: (2017)

Effects of linker and liposome anchoring on lactose-functionalized glycomacromolecules as multivalent ligands for binding galectin-3
por: Freichel, Tanja, et al.
Publicado: (2019)

Galectin-3 and galectin-3 binding protein in rheumatoid arthritis
por: Ohshima, S, et al.
Publicado: (2003)

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
por: Williamson, Rosalind C., et al.
Publicado: (2015)

Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro
Publicado: (1976)

Lactose-Functionalized Carbosilane Glycodendrimers Are Highly Potent Multivalent Ligands for Galectin-9 Binding: Increased Glycan Affinity to Galectins Correlates with Aggregation Behavior
por: Müllerová, Monika, et al.
Publicado: (2023)

Removing nucleic acids from nucleoid-associated proteins purified by affinity column
por: Vingadassalon, Audrey, et al.
Publicado: (2016)

Comprehensive Characterization of (S)GTP-Binding Proteins by Orthogonal Quantitative (S)GTP-Affinity Profiling and (S)GTP/GTP Competition Assays
por: Xiao, Yongsheng, et al.
Publicado: (2014)

Thiodigalactoside–Bovine Serum Albumin Conjugates as High-Potency Inhibitors of Galectin-3: An Outstanding Example of Multivalent Presentation of Small Molecule Inhibitors
por: Zhang, Hao, et al.
Publicado: (2018)

A robust assay to measure DNA topology-dependent protein binding affinity
por: Litwin, Tamara R., et al.
Publicado: (2015)

A Simple Fluorescence Affinity Assay to Decipher Uranyl‐Binding to Native Proteins
por: Laporte, Fanny, et al.
Publicado: (2022)

Rapid Screening of Drug-Protein Binding Using High-Performance Affinity Chromatography with Columns Containing Immobilized Human Serum Albumin
por: Li, Ying-Fei, et al.
Publicado: (2013)

Serum Galectin-9 and Galectin-3-Binding Protein in Acute Dengue Virus Infection
por: Liu, Kuan-Ting, et al.
Publicado: (2016)

Entropy–Entropy Compensation between the Protein, Ligand, and Solvent Degrees of Freedom Fine-Tunes Affinity in Ligand Binding to Galectin-3C
por: Wallerstein, Johan, et al.
Publicado: (2021)

Galectin 3 and Galectin 3 Binding Protein Improve the Risk Stratification after Myocardial Infarction
por: Gagno, Giulia, et al.
Publicado: (2019)

General Approach for Characterizing In Vitro Selected Peptides with Protein Binding Affinity
por: Larsen, Andrew C., et al.
Publicado: (2014)

Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands
por: Richards, Sarah-Jane, et al.
Publicado: (2020)

Identification of VPS13C as a Galectin-12-Binding Protein That Regulates Galectin-12 Protein Stability and Adipogenesis
por: Yang, Ri-Yao, et al.
Publicado: (2016)

Purification of Gst Fusion Proteins, On-Column Cleavage, and Sample Clean Up
por: Sigrell, Jill A., et al.
Publicado: (2002)

Receptor Binding Affinities of Synthetic Cannabinoids Determined by Non-Isotopic Receptor Binding Assay
por: Cha, Hye Jin, et al.
Publicado: (2019)

Serum galectin-3 and galectin-3 binding protein levels in systemic lupus erythematosus and cutaneous lupus erythematosus
por: Kalinska-Bienias, Agnieszka, et al.
Publicado: (2020)

Visualizing Galectin-3 Binding Protein Expression with ImmunoPET
por: Keinänen, Outi, et al.
Publicado: (2023)

Characterizing informative sequence descriptors and predicting binding affinities of heterodimeric protein complexes
por: Srinivasulu, Yerukala Sathipati, et al.
Publicado: (2015)

Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes
por: Masaki, Kanako, et al.
Publicado: (2023)

In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies
por: Boulet-Audet, Maxime, et al.
Publicado: (2016)

Learning protein-ligand binding affinity with atomic environment vectors
por: Meli, Rocco, et al.
Publicado: (2021)

The Binding Affinity and Molecular Basis of the Structure-Binding Relationship between Urinary Tamm-Horsfall Glycoprotein and Tumor Necrosis Factor-α
por: Wu, Cheng-Han, et al.
Publicado: (2012)

Endothelial Galectin-1 Binds to Specific Glycans on Nipah Virus Fusion Protein and Inhibits Maturation, Mobility, and Function to Block Syncytia Formation
por: Garner, Omai B., et al.
Publicado: (2010)

Purification and characterization of mouse serum protein with specific binding affinity for C4 (Ss protein)
por: Ferreira, A, et al.
Publicado: (1977)

Docking Applied to the Prediction of the Affinity of Compounds to P-Glycoprotein
por: Palestro, Pablo H., et al.
Publicado: (2014)

PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity
por: Romero-Molina, Sandra, et al.
Publicado: (2022)

Activation of a Retroviral Membrane Fusion Protein: Soluble Receptor-induced Liposome Binding of the ALSV Envelope Glycoprotein
por: Hernandez, Lorraine D., et al.
Publicado: (1997)

Cannot write session to /tmp/vufind_sessions/sess_jh0nd7pjf4o1vu7ik4sh5fpsgt