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Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage

In this study, endophytic and rhizospheric bacteria were isolated from Moringa olifera and M. perigreina from Oman, and their in vitro antagonistic activity against Pythium aphanidermatum was tested using a dual culture assay. The promising strains were tested further for their compatibility and pot...

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Autores principales: Al-Rahbi, Buthaina Aamir Ali, Al-Sadi, Abdullah Mohammed, Al-Harrasi, Majida Mohammed Ali, Al-Sabahi, Jamal Nasser, Al-Mahmooli, Issa Hashil, Blackburn, Daniel, Velazhahan, Rethinasamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919774/
https://www.ncbi.nlm.nih.gov/pubmed/36771752
http://dx.doi.org/10.3390/plants12030668
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author Al-Rahbi, Buthaina Aamir Ali
Al-Sadi, Abdullah Mohammed
Al-Harrasi, Majida Mohammed Ali
Al-Sabahi, Jamal Nasser
Al-Mahmooli, Issa Hashil
Blackburn, Daniel
Velazhahan, Rethinasamy
author_facet Al-Rahbi, Buthaina Aamir Ali
Al-Sadi, Abdullah Mohammed
Al-Harrasi, Majida Mohammed Ali
Al-Sabahi, Jamal Nasser
Al-Mahmooli, Issa Hashil
Blackburn, Daniel
Velazhahan, Rethinasamy
author_sort Al-Rahbi, Buthaina Aamir Ali
collection PubMed
description In this study, endophytic and rhizospheric bacteria were isolated from Moringa olifera and M. perigreina from Oman, and their in vitro antagonistic activity against Pythium aphanidermatum was tested using a dual culture assay. The promising strains were tested further for their compatibility and potential for plant growth promotion, biofilm formation, antifungal volatile organic compound (VOC) production, and the biological control of P. aphanidermatum damping-off of cabbage (Brassica oleracea L.) under greenhouse conditions. A total of 12 endophytic and 27 rhizospheric bacteria were isolated from Moringa spp. Among them, Bacillus pumilus MPE1 showed the maximum antagonistic activity against P. aphanidermatum in the dual culture assay, followed by Paenibacillus glucanolyticus MPE3 and Pseudomonas indica MOR3 and MOR8. These bacterial isolates induced abundant morphological abnormalities in the hyphae of P. aphanidermatum, as observed via scanning electron microscopy. The in vitro cross-streak assay showed that these bacterial isolates were compatible among themselves, except for P. indica MOR8 × P. glucanolyticus MPE3. These antagonists released VOCs that restricted the growth of P. aphanidermatum in an in vitro assay. These antagonistic bacteria released 2,4-dimethylheptane and 4-methyloctane as the predominant volatile compounds. Of the four antagonistic bacterial strains, P. indica MOR8 was capable of forming biofilm, which is considered a trait that improves the efficacy of rhizosphere inoculants. The results of the greenhouse experiments showed that the soil treatment with B. pumilus MPE1 showed the highest reduction (59%) in the incidence of P. aphanidermatum damping-off in cabbage, evidencing its potential as a biological control agent for the management of this disease. Further research is needed to characterize the antifungal traits and activities of B. pumilus MPE1 and to assert its potential use against other soil-borne plant pathogens.
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spelling pubmed-99197742023-02-12 Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage Al-Rahbi, Buthaina Aamir Ali Al-Sadi, Abdullah Mohammed Al-Harrasi, Majida Mohammed Ali Al-Sabahi, Jamal Nasser Al-Mahmooli, Issa Hashil Blackburn, Daniel Velazhahan, Rethinasamy Plants (Basel) Article In this study, endophytic and rhizospheric bacteria were isolated from Moringa olifera and M. perigreina from Oman, and their in vitro antagonistic activity against Pythium aphanidermatum was tested using a dual culture assay. The promising strains were tested further for their compatibility and potential for plant growth promotion, biofilm formation, antifungal volatile organic compound (VOC) production, and the biological control of P. aphanidermatum damping-off of cabbage (Brassica oleracea L.) under greenhouse conditions. A total of 12 endophytic and 27 rhizospheric bacteria were isolated from Moringa spp. Among them, Bacillus pumilus MPE1 showed the maximum antagonistic activity against P. aphanidermatum in the dual culture assay, followed by Paenibacillus glucanolyticus MPE3 and Pseudomonas indica MOR3 and MOR8. These bacterial isolates induced abundant morphological abnormalities in the hyphae of P. aphanidermatum, as observed via scanning electron microscopy. The in vitro cross-streak assay showed that these bacterial isolates were compatible among themselves, except for P. indica MOR8 × P. glucanolyticus MPE3. These antagonists released VOCs that restricted the growth of P. aphanidermatum in an in vitro assay. These antagonistic bacteria released 2,4-dimethylheptane and 4-methyloctane as the predominant volatile compounds. Of the four antagonistic bacterial strains, P. indica MOR8 was capable of forming biofilm, which is considered a trait that improves the efficacy of rhizosphere inoculants. The results of the greenhouse experiments showed that the soil treatment with B. pumilus MPE1 showed the highest reduction (59%) in the incidence of P. aphanidermatum damping-off in cabbage, evidencing its potential as a biological control agent for the management of this disease. Further research is needed to characterize the antifungal traits and activities of B. pumilus MPE1 and to assert its potential use against other soil-borne plant pathogens. MDPI 2023-02-02 /pmc/articles/PMC9919774/ /pubmed/36771752 http://dx.doi.org/10.3390/plants12030668 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Rahbi, Buthaina Aamir Ali
Al-Sadi, Abdullah Mohammed
Al-Harrasi, Majida Mohammed Ali
Al-Sabahi, Jamal Nasser
Al-Mahmooli, Issa Hashil
Blackburn, Daniel
Velazhahan, Rethinasamy
Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title_full Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title_fullStr Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title_full_unstemmed Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title_short Effectiveness of Endophytic and Rhizospheric Bacteria from Moringa spp. in Controlling Pythium aphanidermatum Damping-Off of Cabbage
title_sort effectiveness of endophytic and rhizospheric bacteria from moringa spp. in controlling pythium aphanidermatum damping-off of cabbage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919774/
https://www.ncbi.nlm.nih.gov/pubmed/36771752
http://dx.doi.org/10.3390/plants12030668
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