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Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion

Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/...

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Autores principales: Busi, Kumar Babu, Das, Subhalaxmi, Palanivel, Mathangi, Ghosh, Krishna Kanta, Gulyás, Balázs, Padmanabhan, Parasuraman, Chakrabortty, Sabyasachi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919789/
https://www.ncbi.nlm.nih.gov/pubmed/36770489
http://dx.doi.org/10.3390/nano13030529
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author Busi, Kumar Babu
Das, Subhalaxmi
Palanivel, Mathangi
Ghosh, Krishna Kanta
Gulyás, Balázs
Padmanabhan, Parasuraman
Chakrabortty, Sabyasachi
author_facet Busi, Kumar Babu
Das, Subhalaxmi
Palanivel, Mathangi
Ghosh, Krishna Kanta
Gulyás, Balázs
Padmanabhan, Parasuraman
Chakrabortty, Sabyasachi
author_sort Busi, Kumar Babu
collection PubMed
description Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/green-color-emitting protein/amino-acid-mediated Cu NCs in an aqueous medium. As-synthesized Cu NCs were characterized through UV-Vis absorption spectroscopy, fluorescence spectroscopy, time-resolved photoluminescence, dynamic light scattering, zeta potential, transmission electron microscopy and X-ray photoelectron spectroscopy. The optical properties of both Cu NCs responded linearly to the variation in pH in the neutral and alkaline ranges, and a robust pH reversible nature (between pH 7 and 11) was observed that could be extended to rapid, localized pH sensor development. However, a contrasting pH response nature between protein–Cu NCs and amino acid–Cu NCs was recorded. The alteration in protein secondary structure and strong binding nature of the surfactants were suggested to explain this behavior. Furthermore, we investigated their use as an efficient optical probe for fluoride ion detection. The limit of detection for protein–Cu NCs is 6.74 µM, whereas the limit of detection for amino acid–Cu NCs is 4.67 µM. Thus, it is anticipated that ultrasmall Cu NCs will exhibit promise in biological and environmental sensing applications.
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spelling pubmed-99197892023-02-12 Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion Busi, Kumar Babu Das, Subhalaxmi Palanivel, Mathangi Ghosh, Krishna Kanta Gulyás, Balázs Padmanabhan, Parasuraman Chakrabortty, Sabyasachi Nanomaterials (Basel) Article Functional metal nanomaterials, especially in the nanocluster (NC) size regime, with strong fluorescence, aqueous colloidal stability, and low toxicity, necessitate their application potential in biology and environmental science. Here, we successfully report a simple cost-effective method for red-/green-color-emitting protein/amino-acid-mediated Cu NCs in an aqueous medium. As-synthesized Cu NCs were characterized through UV-Vis absorption spectroscopy, fluorescence spectroscopy, time-resolved photoluminescence, dynamic light scattering, zeta potential, transmission electron microscopy and X-ray photoelectron spectroscopy. The optical properties of both Cu NCs responded linearly to the variation in pH in the neutral and alkaline ranges, and a robust pH reversible nature (between pH 7 and 11) was observed that could be extended to rapid, localized pH sensor development. However, a contrasting pH response nature between protein–Cu NCs and amino acid–Cu NCs was recorded. The alteration in protein secondary structure and strong binding nature of the surfactants were suggested to explain this behavior. Furthermore, we investigated their use as an efficient optical probe for fluoride ion detection. The limit of detection for protein–Cu NCs is 6.74 µM, whereas the limit of detection for amino acid–Cu NCs is 4.67 µM. Thus, it is anticipated that ultrasmall Cu NCs will exhibit promise in biological and environmental sensing applications. MDPI 2023-01-28 /pmc/articles/PMC9919789/ /pubmed/36770489 http://dx.doi.org/10.3390/nano13030529 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Busi, Kumar Babu
Das, Subhalaxmi
Palanivel, Mathangi
Ghosh, Krishna Kanta
Gulyás, Balázs
Padmanabhan, Parasuraman
Chakrabortty, Sabyasachi
Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title_full Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title_fullStr Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title_full_unstemmed Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title_short Surface Ligand Influences the Cu Nanoclusters as a Dual Sensing Optical Probe for Localized pH Environment and Fluoride Ion
title_sort surface ligand influences the cu nanoclusters as a dual sensing optical probe for localized ph environment and fluoride ion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919789/
https://www.ncbi.nlm.nih.gov/pubmed/36770489
http://dx.doi.org/10.3390/nano13030529
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