Cargando…
Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia
Acacia saligna growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of A. saligna. Bioactive extracts were determined using in vitro antioxidant and yeast α-gl...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919957/ https://www.ncbi.nlm.nih.gov/pubmed/36770694 http://dx.doi.org/10.3390/molecules28031028 |
_version_ | 1784886951890911232 |
---|---|
author | Asmara, Anjar P. Prasansuklab, Anchalee Tencomnao, Tewin Ung, Alison T. |
author_facet | Asmara, Anjar P. Prasansuklab, Anchalee Tencomnao, Tewin Ung, Alison T. |
author_sort | Asmara, Anjar P. |
collection | PubMed |
description | Acacia saligna growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of A. saligna. Bioactive extracts were determined using in vitro antioxidant and yeast α-glucosidase inhibitory assays. Methanolic extracts from barks, leaves, and flowers are the most active and have no toxicity against 3T3-L1 adipocytes. Compound isolation of bioactive extracts provided us with ten compounds. Among them are two novel natural products; naringenin-7-O-α-L-arabinopyranoside 2 and (3S*,5S*)-3-hydroxy-5-(2-aminoethyl) dihydrofuran-2(3H)-one 9. D-(+)-pinitol 5a (from barks and flowers), (−)-pinitol 5b (exclusively from leaf), and 2,4-di-t-butylphenol 7 are known natural products and new to A. saligna. (−)-Epicatechin 6, quercitrin 4, and myricitrin 8 showed potent antioxidant activities consistently in DPPH and ABTS assays. (−)-Epicatechin 6 (IC(50) = 63.58 μM), D-(+)-pinitol 5a (IC(50) = 74.69 μM), and naringenin 1 (IC(50) = 89.71 μM) are the strong inhibitors against the α-glucosidase enzyme. The presence of these compounds supports the activities exerted in our methanolic extracts. The presence of 2,4-di-t-butylphenol 7 may support the reported allelopathic and antifungal activities. The outcome of this study indicates the potential of Australian A. saligna as a rich source of bioactive compounds for drug discovery targeting type 2 diabetes. |
format | Online Article Text |
id | pubmed-9919957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99199572023-02-12 Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia Asmara, Anjar P. Prasansuklab, Anchalee Tencomnao, Tewin Ung, Alison T. Molecules Article Acacia saligna growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of A. saligna. Bioactive extracts were determined using in vitro antioxidant and yeast α-glucosidase inhibitory assays. Methanolic extracts from barks, leaves, and flowers are the most active and have no toxicity against 3T3-L1 adipocytes. Compound isolation of bioactive extracts provided us with ten compounds. Among them are two novel natural products; naringenin-7-O-α-L-arabinopyranoside 2 and (3S*,5S*)-3-hydroxy-5-(2-aminoethyl) dihydrofuran-2(3H)-one 9. D-(+)-pinitol 5a (from barks and flowers), (−)-pinitol 5b (exclusively from leaf), and 2,4-di-t-butylphenol 7 are known natural products and new to A. saligna. (−)-Epicatechin 6, quercitrin 4, and myricitrin 8 showed potent antioxidant activities consistently in DPPH and ABTS assays. (−)-Epicatechin 6 (IC(50) = 63.58 μM), D-(+)-pinitol 5a (IC(50) = 74.69 μM), and naringenin 1 (IC(50) = 89.71 μM) are the strong inhibitors against the α-glucosidase enzyme. The presence of these compounds supports the activities exerted in our methanolic extracts. The presence of 2,4-di-t-butylphenol 7 may support the reported allelopathic and antifungal activities. The outcome of this study indicates the potential of Australian A. saligna as a rich source of bioactive compounds for drug discovery targeting type 2 diabetes. MDPI 2023-01-19 /pmc/articles/PMC9919957/ /pubmed/36770694 http://dx.doi.org/10.3390/molecules28031028 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Asmara, Anjar P. Prasansuklab, Anchalee Tencomnao, Tewin Ung, Alison T. Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title | Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title_full | Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title_fullStr | Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title_full_unstemmed | Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title_short | Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia |
title_sort | identification of phytochemicals in bioactive extracts of acacia saligna growing in australia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9919957/ https://www.ncbi.nlm.nih.gov/pubmed/36770694 http://dx.doi.org/10.3390/molecules28031028 |
work_keys_str_mv | AT asmaraanjarp identificationofphytochemicalsinbioactiveextractsofacaciasalignagrowinginaustralia AT prasansuklabanchalee identificationofphytochemicalsinbioactiveextractsofacaciasalignagrowinginaustralia AT tencomnaotewin identificationofphytochemicalsinbioactiveextractsofacaciasalignagrowinginaustralia AT ungalisont identificationofphytochemicalsinbioactiveextractsofacaciasalignagrowinginaustralia |