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Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS

A synthetic route to producing gold-doped environmentally relevant nanoplastics and a method for the rapid and high-throughput qualitative investigation of their cellular interactions have been developed. Polyethylene (PE) and polyvinyl chloride (PVC) nanoparticles, doped with ultrasmall gold nanopa...

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Autores principales: Cassano, Domenico, Bogni, Alessia, La Spina, Rita, Gilliland, Douglas, Ponti, Jessica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920308/
https://www.ncbi.nlm.nih.gov/pubmed/36770555
http://dx.doi.org/10.3390/nano13030594
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author Cassano, Domenico
Bogni, Alessia
La Spina, Rita
Gilliland, Douglas
Ponti, Jessica
author_facet Cassano, Domenico
Bogni, Alessia
La Spina, Rita
Gilliland, Douglas
Ponti, Jessica
author_sort Cassano, Domenico
collection PubMed
description A synthetic route to producing gold-doped environmentally relevant nanoplastics and a method for the rapid and high-throughput qualitative investigation of their cellular interactions have been developed. Polyethylene (PE) and polyvinyl chloride (PVC) nanoparticles, doped with ultrasmall gold nanoparticles, were synthesized via an oil-in-water emulsion technique as models for floating and sedimenting nanoplastics, respectively. Gold nanoparticles were chosen as a dopant as they are considered to be chemically stable, relatively easy to obtain, interference-free for elemental analysis, and suitable for bio-applications. The suitability of the doped particles for quick detection via inductively coupled plasma mass spectrometry (ICP-MS), operating in single-cell mode (scICP-MS), was demonstrated. Specifically, the method was applied to the analysis of nanoplastics in sizes ranging from 50 to 350 nm, taking advantage of the low limit of detection of single-cell ICP-MS for gold nanoparticles. As an initial proof of concept, gold-doped PVC and PE nanoplastics were employed to quantify the interaction and uptake of nanoplastics by the RAW 264.7 mouse macrophage cell line, using scICP-MS and electron microscopy. Macrophages were chosen because their natural biological functions would make them likely to internalize nanoplastics and, thus, would produce samples to verify the test methodology. Finally, the method was applied to assess the uptake by CaCo-2 human intestinal cells, this being a more relevant model for humanexposure to those nanoplastics that are potentially available in the food chain. For both case studies, two concentrations of nanoplastics were employed to simulate both standard environmental conditions and exceptional circumstances, such as pollution hotspot areas.
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spelling pubmed-99203082023-02-12 Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS Cassano, Domenico Bogni, Alessia La Spina, Rita Gilliland, Douglas Ponti, Jessica Nanomaterials (Basel) Article A synthetic route to producing gold-doped environmentally relevant nanoplastics and a method for the rapid and high-throughput qualitative investigation of their cellular interactions have been developed. Polyethylene (PE) and polyvinyl chloride (PVC) nanoparticles, doped with ultrasmall gold nanoparticles, were synthesized via an oil-in-water emulsion technique as models for floating and sedimenting nanoplastics, respectively. Gold nanoparticles were chosen as a dopant as they are considered to be chemically stable, relatively easy to obtain, interference-free for elemental analysis, and suitable for bio-applications. The suitability of the doped particles for quick detection via inductively coupled plasma mass spectrometry (ICP-MS), operating in single-cell mode (scICP-MS), was demonstrated. Specifically, the method was applied to the analysis of nanoplastics in sizes ranging from 50 to 350 nm, taking advantage of the low limit of detection of single-cell ICP-MS for gold nanoparticles. As an initial proof of concept, gold-doped PVC and PE nanoplastics were employed to quantify the interaction and uptake of nanoplastics by the RAW 264.7 mouse macrophage cell line, using scICP-MS and electron microscopy. Macrophages were chosen because their natural biological functions would make them likely to internalize nanoplastics and, thus, would produce samples to verify the test methodology. Finally, the method was applied to assess the uptake by CaCo-2 human intestinal cells, this being a more relevant model for humanexposure to those nanoplastics that are potentially available in the food chain. For both case studies, two concentrations of nanoplastics were employed to simulate both standard environmental conditions and exceptional circumstances, such as pollution hotspot areas. MDPI 2023-02-01 /pmc/articles/PMC9920308/ /pubmed/36770555 http://dx.doi.org/10.3390/nano13030594 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cassano, Domenico
Bogni, Alessia
La Spina, Rita
Gilliland, Douglas
Ponti, Jessica
Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title_full Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title_fullStr Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title_full_unstemmed Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title_short Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS
title_sort investigating the cellular uptake of model nanoplastics by single-cell icp-ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920308/
https://www.ncbi.nlm.nih.gov/pubmed/36770555
http://dx.doi.org/10.3390/nano13030594
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