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Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids
The use of natural products as chemotherapeutic agents is well established. However, many are associated with undesirable side effects, including high toxicity and instability. Previous reports on the cytotoxic activity of pyrroloiminoquinones isolated from Latrunculid sponges against cancer cell li...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920385/ https://www.ncbi.nlm.nih.gov/pubmed/36770324 http://dx.doi.org/10.3390/ma16031319 |
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author | Mubaiwa, Byron Lerata, Mookho S. Sibuyi, Nicole R. S. Meyer, Mervin Samaai, Toufiek Bolton, John J. Antunes, Edith M. Beukes, Denzil R. |
author_facet | Mubaiwa, Byron Lerata, Mookho S. Sibuyi, Nicole R. S. Meyer, Mervin Samaai, Toufiek Bolton, John J. Antunes, Edith M. Beukes, Denzil R. |
author_sort | Mubaiwa, Byron |
collection | PubMed |
description | The use of natural products as chemotherapeutic agents is well established. However, many are associated with undesirable side effects, including high toxicity and instability. Previous reports on the cytotoxic activity of pyrroloiminoquinones isolated from Latrunculid sponges against cancer cell lines revealed extraordinary activity at IC(50) of 77nM for discorhabdins. Their general lack of selectivity against the cancer and normal cell lines, however, precludes further development. In this study, extraction of a South African Latrunculid sponge produced three known pyrroloiminoquinone metabolites (14-bromodiscorhabdin C (5), Tsitsikammamine A (6) and B (7)). The assignment of the structures was established using standard 1D and 2D NMR experiments. To mitigate the lack of selectivity, the compounds were loaded onto gold nanoparticles synthesized using the aqueous extract of a brown seaweed, Sargassum incisifolium (sAuNPs). The cytotoxicity of the metabolites alone, and their sAuNP conjugates, were evaluated together with the known anticancer agent doxorubicin and its AuNP conjugate. The compound-AuNP conjugates retained their strong cytotoxic activity against the MCF-7 cell line, with >90% of the pyrroloiminoquinone-loaded AuNPs penetrating the cell membrane. Loading cytotoxic natural products onto AuNPs provides an avenue in overcoming some issues hampering the development of new anticancer drugs. |
format | Online Article Text |
id | pubmed-9920385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99203852023-02-12 Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids Mubaiwa, Byron Lerata, Mookho S. Sibuyi, Nicole R. S. Meyer, Mervin Samaai, Toufiek Bolton, John J. Antunes, Edith M. Beukes, Denzil R. Materials (Basel) Article The use of natural products as chemotherapeutic agents is well established. However, many are associated with undesirable side effects, including high toxicity and instability. Previous reports on the cytotoxic activity of pyrroloiminoquinones isolated from Latrunculid sponges against cancer cell lines revealed extraordinary activity at IC(50) of 77nM for discorhabdins. Their general lack of selectivity against the cancer and normal cell lines, however, precludes further development. In this study, extraction of a South African Latrunculid sponge produced three known pyrroloiminoquinone metabolites (14-bromodiscorhabdin C (5), Tsitsikammamine A (6) and B (7)). The assignment of the structures was established using standard 1D and 2D NMR experiments. To mitigate the lack of selectivity, the compounds were loaded onto gold nanoparticles synthesized using the aqueous extract of a brown seaweed, Sargassum incisifolium (sAuNPs). The cytotoxicity of the metabolites alone, and their sAuNP conjugates, were evaluated together with the known anticancer agent doxorubicin and its AuNP conjugate. The compound-AuNP conjugates retained their strong cytotoxic activity against the MCF-7 cell line, with >90% of the pyrroloiminoquinone-loaded AuNPs penetrating the cell membrane. Loading cytotoxic natural products onto AuNPs provides an avenue in overcoming some issues hampering the development of new anticancer drugs. MDPI 2023-02-03 /pmc/articles/PMC9920385/ /pubmed/36770324 http://dx.doi.org/10.3390/ma16031319 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mubaiwa, Byron Lerata, Mookho S. Sibuyi, Nicole R. S. Meyer, Mervin Samaai, Toufiek Bolton, John J. Antunes, Edith M. Beukes, Denzil R. Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title | Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title_full | Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title_fullStr | Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title_full_unstemmed | Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title_short | Green Synthesized sAuNPs as a Potential Delivery Platform for Cytotoxic Alkaloids |
title_sort | green synthesized saunps as a potential delivery platform for cytotoxic alkaloids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920385/ https://www.ncbi.nlm.nih.gov/pubmed/36770324 http://dx.doi.org/10.3390/ma16031319 |
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