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Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva
One of the current challenges facing researchers is the search for alternative biological material, as opposed to routinely and invasively collected (such as blood), as the analysis of the former would provide information about the state of human health, allowing for the diagnosis of diseases in the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920431/ https://www.ncbi.nlm.nih.gov/pubmed/36770757 http://dx.doi.org/10.3390/molecules28031092 |
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author | Dobrowolska-Iwanek, Justyna Jamka-Kasprzyk, Małgorzata Rusin, Marcelina Paśko, Paweł Grekh, Sviatoslav Jurczak, Anna |
author_facet | Dobrowolska-Iwanek, Justyna Jamka-Kasprzyk, Małgorzata Rusin, Marcelina Paśko, Paweł Grekh, Sviatoslav Jurczak, Anna |
author_sort | Dobrowolska-Iwanek, Justyna |
collection | PubMed |
description | One of the current challenges facing researchers is the search for alternative biological material, as opposed to routinely and invasively collected (such as blood), as the analysis of the former would provide information about the state of human health, allowing for the diagnosis of diseases in their early stages. With the search for disease biomarkers in alternative materials, the development of newer analytical solutions has been observed. This study aims to develop a reliable analytical method using the capillary isotachophoresis technique for the determination of organic acids in children’s saliva, the presence/elevation of which can be used in the future for diagnostic purposes. Organic acids such as formic, lactic, acetic, propionic, and butyric acid, were determined in the saliva of healthy children without carious lesions. The limit of quantification determined in the validation process was found to vary from 0.05 to 1.56 mg/L, the recoveries at the two levels were determined to vary between 90% and 110% for level I, while for level II the corresponding values of 75% and 106% were found; the presentation, expressed as relative standard deviation values (RSD), did not exceed 5%. The parameters determined while validating the results method indicated that the obtained are reliable. The Red–Green–Blue (RGB) additive color model was used for the evaluation of the method. This comparative analysis allowed us to define the color of the method, which expresses whether it meets the given assumptions and requirements. According to the RGB model, the isotachophoresis method developed requires less reagent input, shorter sample preparation times, and results with lower energy consumption. Thus, the subject procedure may provide an alternative, routine tool for determining organic acids in human saliva, to be applied in the diagnosing of diseases of various etiological origins. |
format | Online Article Text |
id | pubmed-9920431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99204312023-02-12 Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva Dobrowolska-Iwanek, Justyna Jamka-Kasprzyk, Małgorzata Rusin, Marcelina Paśko, Paweł Grekh, Sviatoslav Jurczak, Anna Molecules Communication One of the current challenges facing researchers is the search for alternative biological material, as opposed to routinely and invasively collected (such as blood), as the analysis of the former would provide information about the state of human health, allowing for the diagnosis of diseases in their early stages. With the search for disease biomarkers in alternative materials, the development of newer analytical solutions has been observed. This study aims to develop a reliable analytical method using the capillary isotachophoresis technique for the determination of organic acids in children’s saliva, the presence/elevation of which can be used in the future for diagnostic purposes. Organic acids such as formic, lactic, acetic, propionic, and butyric acid, were determined in the saliva of healthy children without carious lesions. The limit of quantification determined in the validation process was found to vary from 0.05 to 1.56 mg/L, the recoveries at the two levels were determined to vary between 90% and 110% for level I, while for level II the corresponding values of 75% and 106% were found; the presentation, expressed as relative standard deviation values (RSD), did not exceed 5%. The parameters determined while validating the results method indicated that the obtained are reliable. The Red–Green–Blue (RGB) additive color model was used for the evaluation of the method. This comparative analysis allowed us to define the color of the method, which expresses whether it meets the given assumptions and requirements. According to the RGB model, the isotachophoresis method developed requires less reagent input, shorter sample preparation times, and results with lower energy consumption. Thus, the subject procedure may provide an alternative, routine tool for determining organic acids in human saliva, to be applied in the diagnosing of diseases of various etiological origins. MDPI 2023-01-21 /pmc/articles/PMC9920431/ /pubmed/36770757 http://dx.doi.org/10.3390/molecules28031092 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Dobrowolska-Iwanek, Justyna Jamka-Kasprzyk, Małgorzata Rusin, Marcelina Paśko, Paweł Grekh, Sviatoslav Jurczak, Anna Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title | Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title_full | Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title_fullStr | Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title_full_unstemmed | Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title_short | Developed and Validated Capillary Isotachophoresis Method for the Rapid Determining Organic Acids in Children’s Saliva |
title_sort | developed and validated capillary isotachophoresis method for the rapid determining organic acids in children’s saliva |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920431/ https://www.ncbi.nlm.nih.gov/pubmed/36770757 http://dx.doi.org/10.3390/molecules28031092 |
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