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Intraspecific Variability of Stinging Nettle (Urtica dioica L.)

The purpose of the work was to determine the intraspecific variability of the stinging nettle, in respect of the mass of leaves and their chemical composition, including the content of phenolic compounds and assimilative pigments. The objects of the study were 10 populations of nettle, originating f...

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Autores principales: Koczkodaj, Sylwia, Przybył, Jarosław L., Kosakowska, Olga, Węglarz, Zenon, Bączek, Katarzyna B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921046/
https://www.ncbi.nlm.nih.gov/pubmed/36771171
http://dx.doi.org/10.3390/molecules28031505
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author Koczkodaj, Sylwia
Przybył, Jarosław L.
Kosakowska, Olga
Węglarz, Zenon
Bączek, Katarzyna B.
author_facet Koczkodaj, Sylwia
Przybył, Jarosław L.
Kosakowska, Olga
Węglarz, Zenon
Bączek, Katarzyna B.
author_sort Koczkodaj, Sylwia
collection PubMed
description The purpose of the work was to determine the intraspecific variability of the stinging nettle, in respect of the mass of leaves and their chemical composition, including the content of phenolic compounds and assimilative pigments. The objects of the study were 10 populations of nettle, originating from the eastern and southern part of Poland. The results obtained indicate a high level of variability between and within the populations investigated but not strictly related to their geographical locations. The mass of the leaves ranged from 0.19 to 0.28 kg dry weight (DW)/plant (Coefficient of variation (CV) = 16.33%). Using HPLC–DAD, four phenolic acids were detected, i.e., caffeoylmalic (570.97–1367.40 mg/100 g DW), chlorogenic (352.79–1070.83 mg/100 g DW), neochlorogenic (114.56–284.77 mg/100 g DW) and cichoric (58.31–189.52 mg/100 g DW) acids, with the last one differentiating populations to the highest degree (CV = 48.83%). All of the analyzed populations met the requirements of the European Pharmacopoeia (Ph Eur 10th) concerning the minimum content of caffeoylmalic and chlorogenic acids in nettle leaves (not less than 0.3%). Within the flavonoid fraction, two compounds were identified, namely rutoside (917.05–1937.43 mg/100 g DW, CV = 21.32%) and hyperoside (42.01–289.45 mg/100 g DW; CV = 55.26%). The level of chlorophyll a ranged from 3.82 to 4.49 mg/g DW, chlorophyll b from 1.59 to 2.19 mg/g DW, while the content of carotenoids varied from 2.34 to 2.60 mg/100 g DW. Given all the traits investigated, the level of a population’s polymorphism (CV) was visibly higher within a population than between populations. Population no. 4 was distinguished by the highest mass of leaves, and the highest content of rutoside, while population no. 2 was distinguished by the highest content of hyperoside, caffeoylmalic and chlorogenic acid.
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spelling pubmed-99210462023-02-12 Intraspecific Variability of Stinging Nettle (Urtica dioica L.) Koczkodaj, Sylwia Przybył, Jarosław L. Kosakowska, Olga Węglarz, Zenon Bączek, Katarzyna B. Molecules Article The purpose of the work was to determine the intraspecific variability of the stinging nettle, in respect of the mass of leaves and their chemical composition, including the content of phenolic compounds and assimilative pigments. The objects of the study were 10 populations of nettle, originating from the eastern and southern part of Poland. The results obtained indicate a high level of variability between and within the populations investigated but not strictly related to their geographical locations. The mass of the leaves ranged from 0.19 to 0.28 kg dry weight (DW)/plant (Coefficient of variation (CV) = 16.33%). Using HPLC–DAD, four phenolic acids were detected, i.e., caffeoylmalic (570.97–1367.40 mg/100 g DW), chlorogenic (352.79–1070.83 mg/100 g DW), neochlorogenic (114.56–284.77 mg/100 g DW) and cichoric (58.31–189.52 mg/100 g DW) acids, with the last one differentiating populations to the highest degree (CV = 48.83%). All of the analyzed populations met the requirements of the European Pharmacopoeia (Ph Eur 10th) concerning the minimum content of caffeoylmalic and chlorogenic acids in nettle leaves (not less than 0.3%). Within the flavonoid fraction, two compounds were identified, namely rutoside (917.05–1937.43 mg/100 g DW, CV = 21.32%) and hyperoside (42.01–289.45 mg/100 g DW; CV = 55.26%). The level of chlorophyll a ranged from 3.82 to 4.49 mg/g DW, chlorophyll b from 1.59 to 2.19 mg/g DW, while the content of carotenoids varied from 2.34 to 2.60 mg/100 g DW. Given all the traits investigated, the level of a population’s polymorphism (CV) was visibly higher within a population than between populations. Population no. 4 was distinguished by the highest mass of leaves, and the highest content of rutoside, while population no. 2 was distinguished by the highest content of hyperoside, caffeoylmalic and chlorogenic acid. MDPI 2023-02-03 /pmc/articles/PMC9921046/ /pubmed/36771171 http://dx.doi.org/10.3390/molecules28031505 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koczkodaj, Sylwia
Przybył, Jarosław L.
Kosakowska, Olga
Węglarz, Zenon
Bączek, Katarzyna B.
Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title_full Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title_fullStr Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title_full_unstemmed Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title_short Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
title_sort intraspecific variability of stinging nettle (urtica dioica l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921046/
https://www.ncbi.nlm.nih.gov/pubmed/36771171
http://dx.doi.org/10.3390/molecules28031505
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