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PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response

The emergence of resistance to pathogenic bacteria has resulted from the misuse of antibiotics used in wound treatment. Therefore, nanomaterial-based agents can be used to overcome these limitations. In this study, polycaprolactone (PCL)/gelatin/graphene oxide electrospun nanofibers (PGO) are functi...

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Autores principales: Hamdan, Nazirah, Khodir, Wan Khartini Wan Abdul, Hamid, Shafida Abd, Nasir, Mohd Hamzah Mohd, Hamzah, Ahmad Sazali, Cruz-Maya, Iriczalli, Guarino, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921190/
https://www.ncbi.nlm.nih.gov/pubmed/36770449
http://dx.doi.org/10.3390/nano13030488
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author Hamdan, Nazirah
Khodir, Wan Khartini Wan Abdul
Hamid, Shafida Abd
Nasir, Mohd Hamzah Mohd
Hamzah, Ahmad Sazali
Cruz-Maya, Iriczalli
Guarino, Vincenzo
author_facet Hamdan, Nazirah
Khodir, Wan Khartini Wan Abdul
Hamid, Shafida Abd
Nasir, Mohd Hamzah Mohd
Hamzah, Ahmad Sazali
Cruz-Maya, Iriczalli
Guarino, Vincenzo
author_sort Hamdan, Nazirah
collection PubMed
description The emergence of resistance to pathogenic bacteria has resulted from the misuse of antibiotics used in wound treatment. Therefore, nanomaterial-based agents can be used to overcome these limitations. In this study, polycaprolactone (PCL)/gelatin/graphene oxide electrospun nanofibers (PGO) are functionalized via plasma treatment with the monomeric groups diallylamine (PGO-M1), acrylic acid (PGO-M2), and tert-butyl acrylate (PGO-M3) to enhance the action against bacteria cells. The surface functionalization influences the morphology, surface wettability, mechanical properties, and thermal stability of PGO nanofibers. PGO-M1 and PGO-M2 exhibit good antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas PGO-M3 tends to reduce their antibacterial properties compared to PGO nanofibers. The highest proportion of dead bacteria cells is found on the surface of hydrophilic PGO-M1, whereas live cells are colonized on the surface of hydrophobic PGO-M3. Likewise, PGO-M1 shows a good interaction with L929, which is confirmed by the high levels of adhesion and proliferation with respect to the control. All the results confirm that surface functionalization can be strategically used as a tool to engineer PGO nanofibers with controlled antibacterial properties for the fabrication of highly versatile devices suitable for different applications (e.g., health, environmental pollution).
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spelling pubmed-99211902023-02-12 PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response Hamdan, Nazirah Khodir, Wan Khartini Wan Abdul Hamid, Shafida Abd Nasir, Mohd Hamzah Mohd Hamzah, Ahmad Sazali Cruz-Maya, Iriczalli Guarino, Vincenzo Nanomaterials (Basel) Article The emergence of resistance to pathogenic bacteria has resulted from the misuse of antibiotics used in wound treatment. Therefore, nanomaterial-based agents can be used to overcome these limitations. In this study, polycaprolactone (PCL)/gelatin/graphene oxide electrospun nanofibers (PGO) are functionalized via plasma treatment with the monomeric groups diallylamine (PGO-M1), acrylic acid (PGO-M2), and tert-butyl acrylate (PGO-M3) to enhance the action against bacteria cells. The surface functionalization influences the morphology, surface wettability, mechanical properties, and thermal stability of PGO nanofibers. PGO-M1 and PGO-M2 exhibit good antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas PGO-M3 tends to reduce their antibacterial properties compared to PGO nanofibers. The highest proportion of dead bacteria cells is found on the surface of hydrophilic PGO-M1, whereas live cells are colonized on the surface of hydrophobic PGO-M3. Likewise, PGO-M1 shows a good interaction with L929, which is confirmed by the high levels of adhesion and proliferation with respect to the control. All the results confirm that surface functionalization can be strategically used as a tool to engineer PGO nanofibers with controlled antibacterial properties for the fabrication of highly versatile devices suitable for different applications (e.g., health, environmental pollution). MDPI 2023-01-25 /pmc/articles/PMC9921190/ /pubmed/36770449 http://dx.doi.org/10.3390/nano13030488 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hamdan, Nazirah
Khodir, Wan Khartini Wan Abdul
Hamid, Shafida Abd
Nasir, Mohd Hamzah Mohd
Hamzah, Ahmad Sazali
Cruz-Maya, Iriczalli
Guarino, Vincenzo
PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title_full PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title_fullStr PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title_full_unstemmed PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title_short PCL/Gelatin/Graphene Oxide Electrospun Nanofibers: Effect of Surface Functionalization on In Vitro and Antibacterial Response
title_sort pcl/gelatin/graphene oxide electrospun nanofibers: effect of surface functionalization on in vitro and antibacterial response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921190/
https://www.ncbi.nlm.nih.gov/pubmed/36770449
http://dx.doi.org/10.3390/nano13030488
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