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Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats

BACKGROUND AND AIMS: Diabetic kidney disease (DKD) is a prevalent and intractable microvascular complication of diabetes mellitus (DM), the process of which is closely related to abnormal expression of angiogenesis-regulating factors (ARFs). Stem cell transplantation might be a novel strategy for tr...

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Autores principales: Ni, Yu, Chen, Yuqin, Jiang, Xuheng, Pu, Tao, Zhang, Ling, Li, Shaobin, Hu, Linhong, Bai, Bing, Hu, Tingting, Yu, Limei, Yang, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921454/
https://www.ncbi.nlm.nih.gov/pubmed/36785675
http://dx.doi.org/10.2147/DMSO.S371752
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author Ni, Yu
Chen, Yuqin
Jiang, Xuheng
Pu, Tao
Zhang, Ling
Li, Shaobin
Hu, Linhong
Bai, Bing
Hu, Tingting
Yu, Limei
Yang, Yibin
author_facet Ni, Yu
Chen, Yuqin
Jiang, Xuheng
Pu, Tao
Zhang, Ling
Li, Shaobin
Hu, Linhong
Bai, Bing
Hu, Tingting
Yu, Limei
Yang, Yibin
author_sort Ni, Yu
collection PubMed
description BACKGROUND AND AIMS: Diabetic kidney disease (DKD) is a prevalent and intractable microvascular complication of diabetes mellitus (DM), the process of which is closely related to abnormal expression of angiogenesis-regulating factors (ARFs). Stem cell transplantation might be a novel strategy for treating DKD. This study aims to explore the effect of transplantation of human amniotic mesenchymal stem cells (hAMSCs) on renal microangiopathy in a type 1 DKD rat model (T1DRM). METHODS: Seventy-two rats were randomly divided into three groups, including normal control group, DKD group, and hAMSCs transplantation group. T1DRM was established using a rat tail vein injection of streptozotocin (STZ) (55 mg/kg). hAMSCs were obtained from placental amniotic membranes during cesarean delivery and transplanted at 3 and 4 weeks through penile veins. At 6, 8, and 12 weeks following transplantation, blood glucose levels, renal function, pathological kidney alterations, and the expressions of ARFs’ mRNA and protein were analyzed. RESULTS: In T1DRM, transplanted hAMSCs that were homed at the injured site of kidneys increased ARFs’ expression and decreased blood glucose levels. Compared to the DKD group, the levels of 24-h urinary protein, serum creatinine, urea, and kidney injury molecule-1 (KIM-1) were reduced in hAMSCs transplantation group. In terms of renal pathology such as the degree of basement membrane thickening, hAMSCs transplantation was also less severe than the DKD group, thereby alleviating kidney injury. CONCLUSION: hAMSCs transplantation might ameliorate STZ-induced chronic kidney injury through increasing ARFs’ expression in kidneys and lowering blood glucose levels.
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spelling pubmed-99214542023-02-12 Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats Ni, Yu Chen, Yuqin Jiang, Xuheng Pu, Tao Zhang, Ling Li, Shaobin Hu, Linhong Bai, Bing Hu, Tingting Yu, Limei Yang, Yibin Diabetes Metab Syndr Obes Original Research BACKGROUND AND AIMS: Diabetic kidney disease (DKD) is a prevalent and intractable microvascular complication of diabetes mellitus (DM), the process of which is closely related to abnormal expression of angiogenesis-regulating factors (ARFs). Stem cell transplantation might be a novel strategy for treating DKD. This study aims to explore the effect of transplantation of human amniotic mesenchymal stem cells (hAMSCs) on renal microangiopathy in a type 1 DKD rat model (T1DRM). METHODS: Seventy-two rats were randomly divided into three groups, including normal control group, DKD group, and hAMSCs transplantation group. T1DRM was established using a rat tail vein injection of streptozotocin (STZ) (55 mg/kg). hAMSCs were obtained from placental amniotic membranes during cesarean delivery and transplanted at 3 and 4 weeks through penile veins. At 6, 8, and 12 weeks following transplantation, blood glucose levels, renal function, pathological kidney alterations, and the expressions of ARFs’ mRNA and protein were analyzed. RESULTS: In T1DRM, transplanted hAMSCs that were homed at the injured site of kidneys increased ARFs’ expression and decreased blood glucose levels. Compared to the DKD group, the levels of 24-h urinary protein, serum creatinine, urea, and kidney injury molecule-1 (KIM-1) were reduced in hAMSCs transplantation group. In terms of renal pathology such as the degree of basement membrane thickening, hAMSCs transplantation was also less severe than the DKD group, thereby alleviating kidney injury. CONCLUSION: hAMSCs transplantation might ameliorate STZ-induced chronic kidney injury through increasing ARFs’ expression in kidneys and lowering blood glucose levels. Dove 2023-02-07 /pmc/articles/PMC9921454/ /pubmed/36785675 http://dx.doi.org/10.2147/DMSO.S371752 Text en © 2023 Ni et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Ni, Yu
Chen, Yuqin
Jiang, Xuheng
Pu, Tao
Zhang, Ling
Li, Shaobin
Hu, Linhong
Bai, Bing
Hu, Tingting
Yu, Limei
Yang, Yibin
Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title_full Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title_fullStr Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title_full_unstemmed Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title_short Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
title_sort transplantation of human amniotic mesenchymal stem cells up-regulates angiogenic factor expression to attenuate diabetic kidney disease in rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921454/
https://www.ncbi.nlm.nih.gov/pubmed/36785675
http://dx.doi.org/10.2147/DMSO.S371752
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