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Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines

(1) Background: Malignant gliomas are aggressive tumors characterized by fast cellular growth and highly invasive properties. Despite all biological and clinical advances in therapy, the standard treatment remains essentially palliative. Therefore, searching for alternative therapies that minimize a...

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Autores principales: Silva, Viviane A. O., Rosa, Marcela N., Gomes, Izabela N. F., Vital, Patrik da Silva, Alves, Ana Laura V., Evangelista, Adriane F., Longato, Giovanna B., Carloni, Adriana C., Oliveira, Bruno G., Pinto, Fernanda E., Romão, Wanderson, Rezende, Allisson R., Araújo, Arali A. C., Oliveira, Lohanna S. F. M., Souza, Alessandra A. M., Oliveira, Stephanie C., Ribeiro, Rosy Iara Maciel A., Reis, Rui M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921846/
https://www.ncbi.nlm.nih.gov/pubmed/36771057
http://dx.doi.org/10.3390/molecules28031394
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author Silva, Viviane A. O.
Rosa, Marcela N.
Gomes, Izabela N. F.
Vital, Patrik da Silva
Alves, Ana Laura V.
Evangelista, Adriane F.
Longato, Giovanna B.
Carloni, Adriana C.
Oliveira, Bruno G.
Pinto, Fernanda E.
Romão, Wanderson
Rezende, Allisson R.
Araújo, Arali A. C.
Oliveira, Lohanna S. F. M.
Souza, Alessandra A. M.
Oliveira, Stephanie C.
Ribeiro, Rosy Iara Maciel A.
Reis, Rui M.
author_facet Silva, Viviane A. O.
Rosa, Marcela N.
Gomes, Izabela N. F.
Vital, Patrik da Silva
Alves, Ana Laura V.
Evangelista, Adriane F.
Longato, Giovanna B.
Carloni, Adriana C.
Oliveira, Bruno G.
Pinto, Fernanda E.
Romão, Wanderson
Rezende, Allisson R.
Araújo, Arali A. C.
Oliveira, Lohanna S. F. M.
Souza, Alessandra A. M.
Oliveira, Stephanie C.
Ribeiro, Rosy Iara Maciel A.
Reis, Rui M.
author_sort Silva, Viviane A. O.
collection PubMed
description (1) Background: Malignant gliomas are aggressive tumors characterized by fast cellular growth and highly invasive properties. Despite all biological and clinical advances in therapy, the standard treatment remains essentially palliative. Therefore, searching for alternative therapies that minimize adverse symptoms and improve glioblastoma patients’ outcomes is imperative. Natural products represent an essential source in the discovery of such new drugs. Plants from the cerrado biome have been receiving increased attention due to the presence of secondary metabolites with significant therapeutic potential. (2) Aim: This study provides data on the cytotoxic potential of 13 leaf extracts obtained from plants of 5 families (Anacardiaceae, Annonaceae, Fabaceae, Melastomataceae e Siparunaceae) found in the Brazilian cerrado biome on a panel of 5 glioma cell lines and one normal astrocyte. (3) Methods: The effect of crude extracts on cell viability was evaluated by MTS assay. Mass spectrometry (ESI FT-ICR MS) was performed to identify the secondary metabolites classes presented in the crude extracts and partitions. (4) Results: Our results revealed the cytotoxic potential of Melastomataceae species Miconia cuspidata, Miconia albicans, and Miconia chamissois. Additionally, comparing the four partitions obtained from M. chamissois crude extract indicates that the chloroform partition had the greatest cytotoxic activity against the glioma cell lines. The partitions also showed a mean IC(50) close to chemotherapy, temozolomide; nevertheless, lower toxicity against normal astrocytes. Analysis of secondary metabolites classes presented in these crude extracts and partitions indicates the presence of phenolic compounds. (5) Conclusions: These findings highlight M. chamissois chloroform partition as a promising component and may guide the search for the development of additional new anticancer therapies.
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spelling pubmed-99218462023-02-12 Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines Silva, Viviane A. O. Rosa, Marcela N. Gomes, Izabela N. F. Vital, Patrik da Silva Alves, Ana Laura V. Evangelista, Adriane F. Longato, Giovanna B. Carloni, Adriana C. Oliveira, Bruno G. Pinto, Fernanda E. Romão, Wanderson Rezende, Allisson R. Araújo, Arali A. C. Oliveira, Lohanna S. F. M. Souza, Alessandra A. M. Oliveira, Stephanie C. Ribeiro, Rosy Iara Maciel A. Reis, Rui M. Molecules Article (1) Background: Malignant gliomas are aggressive tumors characterized by fast cellular growth and highly invasive properties. Despite all biological and clinical advances in therapy, the standard treatment remains essentially palliative. Therefore, searching for alternative therapies that minimize adverse symptoms and improve glioblastoma patients’ outcomes is imperative. Natural products represent an essential source in the discovery of such new drugs. Plants from the cerrado biome have been receiving increased attention due to the presence of secondary metabolites with significant therapeutic potential. (2) Aim: This study provides data on the cytotoxic potential of 13 leaf extracts obtained from plants of 5 families (Anacardiaceae, Annonaceae, Fabaceae, Melastomataceae e Siparunaceae) found in the Brazilian cerrado biome on a panel of 5 glioma cell lines and one normal astrocyte. (3) Methods: The effect of crude extracts on cell viability was evaluated by MTS assay. Mass spectrometry (ESI FT-ICR MS) was performed to identify the secondary metabolites classes presented in the crude extracts and partitions. (4) Results: Our results revealed the cytotoxic potential of Melastomataceae species Miconia cuspidata, Miconia albicans, and Miconia chamissois. Additionally, comparing the four partitions obtained from M. chamissois crude extract indicates that the chloroform partition had the greatest cytotoxic activity against the glioma cell lines. The partitions also showed a mean IC(50) close to chemotherapy, temozolomide; nevertheless, lower toxicity against normal astrocytes. Analysis of secondary metabolites classes presented in these crude extracts and partitions indicates the presence of phenolic compounds. (5) Conclusions: These findings highlight M. chamissois chloroform partition as a promising component and may guide the search for the development of additional new anticancer therapies. MDPI 2023-02-01 /pmc/articles/PMC9921846/ /pubmed/36771057 http://dx.doi.org/10.3390/molecules28031394 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Viviane A. O.
Rosa, Marcela N.
Gomes, Izabela N. F.
Vital, Patrik da Silva
Alves, Ana Laura V.
Evangelista, Adriane F.
Longato, Giovanna B.
Carloni, Adriana C.
Oliveira, Bruno G.
Pinto, Fernanda E.
Romão, Wanderson
Rezende, Allisson R.
Araújo, Arali A. C.
Oliveira, Lohanna S. F. M.
Souza, Alessandra A. M.
Oliveira, Stephanie C.
Ribeiro, Rosy Iara Maciel A.
Reis, Rui M.
Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title_full Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title_fullStr Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title_full_unstemmed Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title_short Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines
title_sort bio-prospecting of crude leaf extracts from thirteen plants of brazilian cerrado biome on human glioma cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921846/
https://www.ncbi.nlm.nih.gov/pubmed/36771057
http://dx.doi.org/10.3390/molecules28031394
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