Cargando…
A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique
The aim of the current research was to develop a simple and rapid mass spectrometry-based assay for the determination of 15 steroid hormones in human plasma in a single run, which would be suitable for a routine practice setting. For this purpose, we designed a procedure based on the 2D-liquid chrom...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921923/ https://www.ncbi.nlm.nih.gov/pubmed/36771043 http://dx.doi.org/10.3390/molecules28031379 |
_version_ | 1784887428585095168 |
---|---|
author | Kotasova, Marcela Lacina, Ondrej Springer, Drahomira Sevcik, Jan Brutvan, Tomas Jezkova, Jana Zima, Tomas |
author_facet | Kotasova, Marcela Lacina, Ondrej Springer, Drahomira Sevcik, Jan Brutvan, Tomas Jezkova, Jana Zima, Tomas |
author_sort | Kotasova, Marcela |
collection | PubMed |
description | The aim of the current research was to develop a simple and rapid mass spectrometry-based assay for the determination of 15 steroid hormones in human plasma in a single run, which would be suitable for a routine practice setting. For this purpose, we designed a procedure based on the 2D-liquid chromatography-tandem mass spectrometry with a minimalistic sample pre-treatment. In our arrangement, the preparation of one sample takes only 10 min and can accommodate 40 samples per hour when tested in series. The following analytical run is 18 min long for all steroid hormones. In addition, we developed an independent analytical run for estradiol, significantly increasing the assay accuracy while taking an additional 10 min to perform an analytical run of a sample. The optimized method was applied to a set of human plasma samples, including chylous. Our results indicate the linearity of the method for all steroid hormones with squared regression coefficients R(2) ≥ 0.995, within-run and between-run precision (RSD < 6.4%), and an accuracy of 92.9% to 106.2%. The absolute recovery for each analyzed steroid hormone ranged between 101.6% and 116.5%. The method detection limit for 15 steroid hormones ranged between 0.008 nmol/L (2.88 pg/mL) for aldosterone and 0.873 nmol/L (0.252 ng/mL) for DHEA. For all the analytes, the lowest calibration point relative standard deviation was less than 10.8%, indicating a good precision of the assay within the lowest concentration of interest. In conclusion, in this method article, we describe a simple, sensitive, and cost-effective 2D-LC/MS/MS method suitable for the routine analysis of a complex of steroid hormones allowing high analytical specificity and sensitivity despite minimal sample processing and short throughput times. |
format | Online Article Text |
id | pubmed-9921923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99219232023-02-12 A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique Kotasova, Marcela Lacina, Ondrej Springer, Drahomira Sevcik, Jan Brutvan, Tomas Jezkova, Jana Zima, Tomas Molecules Article The aim of the current research was to develop a simple and rapid mass spectrometry-based assay for the determination of 15 steroid hormones in human plasma in a single run, which would be suitable for a routine practice setting. For this purpose, we designed a procedure based on the 2D-liquid chromatography-tandem mass spectrometry with a minimalistic sample pre-treatment. In our arrangement, the preparation of one sample takes only 10 min and can accommodate 40 samples per hour when tested in series. The following analytical run is 18 min long for all steroid hormones. In addition, we developed an independent analytical run for estradiol, significantly increasing the assay accuracy while taking an additional 10 min to perform an analytical run of a sample. The optimized method was applied to a set of human plasma samples, including chylous. Our results indicate the linearity of the method for all steroid hormones with squared regression coefficients R(2) ≥ 0.995, within-run and between-run precision (RSD < 6.4%), and an accuracy of 92.9% to 106.2%. The absolute recovery for each analyzed steroid hormone ranged between 101.6% and 116.5%. The method detection limit for 15 steroid hormones ranged between 0.008 nmol/L (2.88 pg/mL) for aldosterone and 0.873 nmol/L (0.252 ng/mL) for DHEA. For all the analytes, the lowest calibration point relative standard deviation was less than 10.8%, indicating a good precision of the assay within the lowest concentration of interest. In conclusion, in this method article, we describe a simple, sensitive, and cost-effective 2D-LC/MS/MS method suitable for the routine analysis of a complex of steroid hormones allowing high analytical specificity and sensitivity despite minimal sample processing and short throughput times. MDPI 2023-02-01 /pmc/articles/PMC9921923/ /pubmed/36771043 http://dx.doi.org/10.3390/molecules28031379 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kotasova, Marcela Lacina, Ondrej Springer, Drahomira Sevcik, Jan Brutvan, Tomas Jezkova, Jana Zima, Tomas A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title | A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title_full | A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title_fullStr | A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title_full_unstemmed | A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title_short | A New Heart-Cutting Method for a Multiplex Quantitative Analysis of Steroid Hormones in Plasma Using 2D-LC/MS/MS Technique |
title_sort | new heart-cutting method for a multiplex quantitative analysis of steroid hormones in plasma using 2d-lc/ms/ms technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921923/ https://www.ncbi.nlm.nih.gov/pubmed/36771043 http://dx.doi.org/10.3390/molecules28031379 |
work_keys_str_mv | AT kotasovamarcela anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT lacinaondrej anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT springerdrahomira anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT sevcikjan anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT brutvantomas anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT jezkovajana anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT zimatomas anewheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT kotasovamarcela newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT lacinaondrej newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT springerdrahomira newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT sevcikjan newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT brutvantomas newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT jezkovajana newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique AT zimatomas newheartcuttingmethodforamultiplexquantitativeanalysisofsteroidhormonesinplasmausing2dlcmsmstechnique |