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A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples

A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions...

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Autores principales: Jornet-Martínez, Neus, Biosca-Micó, Josep, Campíns-Falcó, Pilar, Herráez-Hernández, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921926/
https://www.ncbi.nlm.nih.gov/pubmed/36770970
http://dx.doi.org/10.3390/molecules28031303
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author Jornet-Martínez, Neus
Biosca-Micó, Josep
Campíns-Falcó, Pilar
Herráez-Hernández, Rosa
author_facet Jornet-Martínez, Neus
Biosca-Micó, Josep
Campíns-Falcó, Pilar
Herráez-Hernández, Rosa
author_sort Jornet-Martínez, Neus
collection PubMed
description A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids Δ(9)-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids.
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spelling pubmed-99219262023-02-12 A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples Jornet-Martínez, Neus Biosca-Micó, Josep Campíns-Falcó, Pilar Herráez-Hernández, Rosa Molecules Article A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids Δ(9)-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids. MDPI 2023-01-30 /pmc/articles/PMC9921926/ /pubmed/36770970 http://dx.doi.org/10.3390/molecules28031303 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jornet-Martínez, Neus
Biosca-Micó, Josep
Campíns-Falcó, Pilar
Herráez-Hernández, Rosa
A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title_full A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title_fullStr A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title_full_unstemmed A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title_short A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
title_sort colorimetric method for the rapid estimation of the total cannabinoid content in cannabis samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921926/
https://www.ncbi.nlm.nih.gov/pubmed/36770970
http://dx.doi.org/10.3390/molecules28031303
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