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A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples
A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921926/ https://www.ncbi.nlm.nih.gov/pubmed/36770970 http://dx.doi.org/10.3390/molecules28031303 |
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author | Jornet-Martínez, Neus Biosca-Micó, Josep Campíns-Falcó, Pilar Herráez-Hernández, Rosa |
author_facet | Jornet-Martínez, Neus Biosca-Micó, Josep Campíns-Falcó, Pilar Herráez-Hernández, Rosa |
author_sort | Jornet-Martínez, Neus |
collection | PubMed |
description | A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids Δ(9)-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids. |
format | Online Article Text |
id | pubmed-9921926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99219262023-02-12 A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples Jornet-Martínez, Neus Biosca-Micó, Josep Campíns-Falcó, Pilar Herráez-Hernández, Rosa Molecules Article A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids Δ(9)-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids. MDPI 2023-01-30 /pmc/articles/PMC9921926/ /pubmed/36770970 http://dx.doi.org/10.3390/molecules28031303 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jornet-Martínez, Neus Biosca-Micó, Josep Campíns-Falcó, Pilar Herráez-Hernández, Rosa A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title | A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title_full | A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title_fullStr | A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title_full_unstemmed | A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title_short | A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples |
title_sort | colorimetric method for the rapid estimation of the total cannabinoid content in cannabis samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921926/ https://www.ncbi.nlm.nih.gov/pubmed/36770970 http://dx.doi.org/10.3390/molecules28031303 |
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