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Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias

BACKGROUND: Dorsal root ganglion stimulation (DRGS) may serve as a novel neuromodulation strategy to reduce cardiac sympathoexcitation and ventricular excitability. OBJECTIVE: In this pre-clinical study, we investigated the effectiveness of DRGS on reducing ventricular arrhythmias and modulating car...

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Autores principales: Kuwabara, Yuki, Howard-Quijano, Kimberly, Salavatian, Siamak, Yamaguchi, Tomoki, Saba, Samir, Mahajan, Aman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9922704/
https://www.ncbi.nlm.nih.gov/pubmed/36793544
http://dx.doi.org/10.3389/fnins.2023.1091230
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author Kuwabara, Yuki
Howard-Quijano, Kimberly
Salavatian, Siamak
Yamaguchi, Tomoki
Saba, Samir
Mahajan, Aman
author_facet Kuwabara, Yuki
Howard-Quijano, Kimberly
Salavatian, Siamak
Yamaguchi, Tomoki
Saba, Samir
Mahajan, Aman
author_sort Kuwabara, Yuki
collection PubMed
description BACKGROUND: Dorsal root ganglion stimulation (DRGS) may serve as a novel neuromodulation strategy to reduce cardiac sympathoexcitation and ventricular excitability. OBJECTIVE: In this pre-clinical study, we investigated the effectiveness of DRGS on reducing ventricular arrhythmias and modulating cardiac sympathetic hyperactivity caused by myocardial ischemia. METHODS: Twenty-three Yorkshire pigs were randomized to two groups, which was control LAD ischemia-reperfusion (CONTROL) or LAD ischemia-reperfusion + DRGS (DRGS) group. In the DRGS group (n = 10), high-frequency stimulation (1 kHz) at the second thoracic level (T2) was initiated 30 min before ischemia and continued throughout 1 h of ischemia and 2 h of reperfusion. Cardiac electrophysiological mapping and Ventricular Arrhythmia Score (VAS) were assessed, along with evaluation of cFos expression and apoptosis in the T2 spinal cord and DRG. RESULTS: DRGS decreased the magnitude of activation recovery interval (ARI) shortening in the ischemic region (CONTROL: −201 ± 9.8 ms, DRGS: −170 ± 9.4 ms, p = 0.0373) and decreased global dispersion of repolarization (DOR) at 30 min of myocardial ischemia (CONTROL: 9546 ± 763 ms(2), DRGS: 6491 ± 636 ms(2), p = 0.0076). DRGS also decreased ventricular arrhythmias (VAS–CONTROL: 8.9 ± 1.1, DRGS: 6.3 ± 1.0, p = 0.038). Immunohistochemistry studies showed that DRGS decreased % cFos with NeuN expression in the T2 spinal cord (p = 0.048) and the number of apoptotic cells in the DRG (p = 0.0084). CONCLUSION: DRGS reduced the burden of myocardial ischemia-induced cardiac sympathoexcitation and has a potential to be a novel treatment option to reduce arrhythmogenesis.
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spelling pubmed-99227042023-02-14 Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias Kuwabara, Yuki Howard-Quijano, Kimberly Salavatian, Siamak Yamaguchi, Tomoki Saba, Samir Mahajan, Aman Front Neurosci Neuroscience BACKGROUND: Dorsal root ganglion stimulation (DRGS) may serve as a novel neuromodulation strategy to reduce cardiac sympathoexcitation and ventricular excitability. OBJECTIVE: In this pre-clinical study, we investigated the effectiveness of DRGS on reducing ventricular arrhythmias and modulating cardiac sympathetic hyperactivity caused by myocardial ischemia. METHODS: Twenty-three Yorkshire pigs were randomized to two groups, which was control LAD ischemia-reperfusion (CONTROL) or LAD ischemia-reperfusion + DRGS (DRGS) group. In the DRGS group (n = 10), high-frequency stimulation (1 kHz) at the second thoracic level (T2) was initiated 30 min before ischemia and continued throughout 1 h of ischemia and 2 h of reperfusion. Cardiac electrophysiological mapping and Ventricular Arrhythmia Score (VAS) were assessed, along with evaluation of cFos expression and apoptosis in the T2 spinal cord and DRG. RESULTS: DRGS decreased the magnitude of activation recovery interval (ARI) shortening in the ischemic region (CONTROL: −201 ± 9.8 ms, DRGS: −170 ± 9.4 ms, p = 0.0373) and decreased global dispersion of repolarization (DOR) at 30 min of myocardial ischemia (CONTROL: 9546 ± 763 ms(2), DRGS: 6491 ± 636 ms(2), p = 0.0076). DRGS also decreased ventricular arrhythmias (VAS–CONTROL: 8.9 ± 1.1, DRGS: 6.3 ± 1.0, p = 0.038). Immunohistochemistry studies showed that DRGS decreased % cFos with NeuN expression in the T2 spinal cord (p = 0.048) and the number of apoptotic cells in the DRG (p = 0.0084). CONCLUSION: DRGS reduced the burden of myocardial ischemia-induced cardiac sympathoexcitation and has a potential to be a novel treatment option to reduce arrhythmogenesis. Frontiers Media S.A. 2023-01-30 /pmc/articles/PMC9922704/ /pubmed/36793544 http://dx.doi.org/10.3389/fnins.2023.1091230 Text en Copyright © 2023 Kuwabara, Howard-Quijano, Salavatian, Yamaguchi, Saba and Mahajan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Kuwabara, Yuki
Howard-Quijano, Kimberly
Salavatian, Siamak
Yamaguchi, Tomoki
Saba, Samir
Mahajan, Aman
Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title_full Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title_fullStr Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title_full_unstemmed Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title_short Thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
title_sort thoracic dorsal root ganglion stimulation reduces acute myocardial ischemia induced ventricular arrhythmias
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9922704/
https://www.ncbi.nlm.nih.gov/pubmed/36793544
http://dx.doi.org/10.3389/fnins.2023.1091230
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