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Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion

BACKGROUND: Oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modificat...

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Autores principales: Qin, Zhiming, Bai, Jiaying, He, Huiying, Li, Binbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923020/
https://www.ncbi.nlm.nih.gov/pubmed/36793593
http://dx.doi.org/10.3389/fonc.2023.1013054
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author Qin, Zhiming
Bai, Jiaying
He, Huiying
Li, Binbin
author_facet Qin, Zhiming
Bai, Jiaying
He, Huiying
Li, Binbin
author_sort Qin, Zhiming
collection PubMed
description BACKGROUND: Oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modification of both coding mRNA and non-coding ncRNA in eukaryotes, participates in the regulation of the occurrence and development of various malignant tumors in human. However, its role in oral epithelial dysplasia (OED) and OSCC remain unclear. MATERIALS AND METHODS: In this study, multiple public databases were used for bioinformatics analysis of 23 common m6A methylation regulators in head and neck squamous cell carcinoma (HNSCC). Protein expressions of IGF2BP2 and IGF2BP3 were verified accordingly in clinical cohort samples of OED and OSCC. RESULTS: Patients with high expression of FTO、HNRNPC、HNRNPA2B1、LRPPRC、IGF2BP1、IGF2BP2、IGF2BP3 had a poor prognosis. IGF2BP2 had a relatively high mutation rate in HNSCC, and its expression was significantly positively correlated with tumor purity, and significantly negatively correlated with the infiltration level of B cells and CD8+T cells. The expression of IGF2BP3 was significantly positively correlated with tumor purity and CD4+T cells. Immunohistochemistrically, the expression of IGF2BP2 and IGF2BP3 in oral simple epithelial hyperplasia, OED and OSCC increased gradually. Both were strongly expressed in OSCC. CONCLUSION: IGF2BP2 and IGF2BP3 were the potential biological prognostic indicators of OED and OSCC.
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spelling pubmed-99230202023-02-14 Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion Qin, Zhiming Bai, Jiaying He, Huiying Li, Binbin Front Oncol Oncology BACKGROUND: Oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modification of both coding mRNA and non-coding ncRNA in eukaryotes, participates in the regulation of the occurrence and development of various malignant tumors in human. However, its role in oral epithelial dysplasia (OED) and OSCC remain unclear. MATERIALS AND METHODS: In this study, multiple public databases were used for bioinformatics analysis of 23 common m6A methylation regulators in head and neck squamous cell carcinoma (HNSCC). Protein expressions of IGF2BP2 and IGF2BP3 were verified accordingly in clinical cohort samples of OED and OSCC. RESULTS: Patients with high expression of FTO、HNRNPC、HNRNPA2B1、LRPPRC、IGF2BP1、IGF2BP2、IGF2BP3 had a poor prognosis. IGF2BP2 had a relatively high mutation rate in HNSCC, and its expression was significantly positively correlated with tumor purity, and significantly negatively correlated with the infiltration level of B cells and CD8+T cells. The expression of IGF2BP3 was significantly positively correlated with tumor purity and CD4+T cells. Immunohistochemistrically, the expression of IGF2BP2 and IGF2BP3 in oral simple epithelial hyperplasia, OED and OSCC increased gradually. Both were strongly expressed in OSCC. CONCLUSION: IGF2BP2 and IGF2BP3 were the potential biological prognostic indicators of OED and OSCC. Frontiers Media S.A. 2023-01-30 /pmc/articles/PMC9923020/ /pubmed/36793593 http://dx.doi.org/10.3389/fonc.2023.1013054 Text en Copyright © 2023 Qin, Bai, He and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Qin, Zhiming
Bai, Jiaying
He, Huiying
Li, Binbin
Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title_full Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title_fullStr Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title_full_unstemmed Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title_short Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
title_sort expression and significance of m6a-rna-methylation in oral cancer and precancerous lesion
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923020/
https://www.ncbi.nlm.nih.gov/pubmed/36793593
http://dx.doi.org/10.3389/fonc.2023.1013054
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