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Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection

The movement protein (MP) and coat protein (CP) of tobamoviruses play critical roles in viral cell‐to‐cell and long‐distance movement, respectively. Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus. The functions of CGMMV MP and CP during viral infection remain largely...

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Autores principales: Shi, Ya‐Juan, Yang, Xue, Yang, Ling‐Ling, Li, Qing‐Lun, Liu, Xiao‐Min, Han, Xiao‐Yu, Gu, Qin‐Sheng, Li, Hong‐Lian, Chen, Lin‐Lin, Liu, Yiqing, Shi, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923391/
https://www.ncbi.nlm.nih.gov/pubmed/36528386
http://dx.doi.org/10.1111/mpp.13287
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author Shi, Ya‐Juan
Yang, Xue
Yang, Ling‐Ling
Li, Qing‐Lun
Liu, Xiao‐Min
Han, Xiao‐Yu
Gu, Qin‐Sheng
Li, Hong‐Lian
Chen, Lin‐Lin
Liu, Yiqing
Shi, Yan
author_facet Shi, Ya‐Juan
Yang, Xue
Yang, Ling‐Ling
Li, Qing‐Lun
Liu, Xiao‐Min
Han, Xiao‐Yu
Gu, Qin‐Sheng
Li, Hong‐Lian
Chen, Lin‐Lin
Liu, Yiqing
Shi, Yan
author_sort Shi, Ya‐Juan
collection PubMed
description The movement protein (MP) and coat protein (CP) of tobamoviruses play critical roles in viral cell‐to‐cell and long‐distance movement, respectively. Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus. The functions of CGMMV MP and CP during viral infection remain largely unclear. Here, we show that CGMMV MP can interact with CP in vivo, and the amino acids at positions 79–128 in MP are vital for the MP–CP interaction. To confirm this finding, we mutated five conserved residues within the residue 79–128 region and six other conserved residues flanking this region, followed by in vivo interaction assays. The results showed that the conserved threonine residue at the position 107 in MP (MP(T107)) is important for the MP–CP interaction. Substitution of T107 with alanine (MP(T107A)) delayed CGMMV systemic infection in Nicotiana benthamiana plants, but increased CGMMV local accumulation. Substitutions of another 10 conserved residues, not responsible for the MP–CP interaction, with alanine inhibited or abolished CGMMV systemic infection, suggesting that these 10 conserved residues are possibly required for the MP movement function through a CP‐independent manner. Moreover, two movement function‐associated point mutants (MP(F17A) and MP(D97A)) failed to cause systemic infection in plants without impacting on the MP–CP interaction. Furthermore, we have found that co‐expression of CGMMV MP and CP increased CP accumulation independent of the interaction. MP and CP interaction inhibits the salicylic acid‐associated defence response at an early infection stage. Taken together, we propose that the suppression of host antiviral defence through the MP–CP interaction facilitates virus systemic infection.
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spelling pubmed-99233912023-02-14 Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection Shi, Ya‐Juan Yang, Xue Yang, Ling‐Ling Li, Qing‐Lun Liu, Xiao‐Min Han, Xiao‐Yu Gu, Qin‐Sheng Li, Hong‐Lian Chen, Lin‐Lin Liu, Yiqing Shi, Yan Mol Plant Pathol Original Articles The movement protein (MP) and coat protein (CP) of tobamoviruses play critical roles in viral cell‐to‐cell and long‐distance movement, respectively. Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus. The functions of CGMMV MP and CP during viral infection remain largely unclear. Here, we show that CGMMV MP can interact with CP in vivo, and the amino acids at positions 79–128 in MP are vital for the MP–CP interaction. To confirm this finding, we mutated five conserved residues within the residue 79–128 region and six other conserved residues flanking this region, followed by in vivo interaction assays. The results showed that the conserved threonine residue at the position 107 in MP (MP(T107)) is important for the MP–CP interaction. Substitution of T107 with alanine (MP(T107A)) delayed CGMMV systemic infection in Nicotiana benthamiana plants, but increased CGMMV local accumulation. Substitutions of another 10 conserved residues, not responsible for the MP–CP interaction, with alanine inhibited or abolished CGMMV systemic infection, suggesting that these 10 conserved residues are possibly required for the MP movement function through a CP‐independent manner. Moreover, two movement function‐associated point mutants (MP(F17A) and MP(D97A)) failed to cause systemic infection in plants without impacting on the MP–CP interaction. Furthermore, we have found that co‐expression of CGMMV MP and CP increased CP accumulation independent of the interaction. MP and CP interaction inhibits the salicylic acid‐associated defence response at an early infection stage. Taken together, we propose that the suppression of host antiviral defence through the MP–CP interaction facilitates virus systemic infection. John Wiley and Sons Inc. 2022-12-17 /pmc/articles/PMC9923391/ /pubmed/36528386 http://dx.doi.org/10.1111/mpp.13287 Text en © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Shi, Ya‐Juan
Yang, Xue
Yang, Ling‐Ling
Li, Qing‐Lun
Liu, Xiao‐Min
Han, Xiao‐Yu
Gu, Qin‐Sheng
Li, Hong‐Lian
Chen, Lin‐Lin
Liu, Yiqing
Shi, Yan
Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title_full Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title_fullStr Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title_full_unstemmed Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title_short Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection
title_sort interaction between cucumber green mottle mosaic virus mp and cp promotes virus systemic infection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923391/
https://www.ncbi.nlm.nih.gov/pubmed/36528386
http://dx.doi.org/10.1111/mpp.13287
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